Whole Genome Sequence Analysis of Lactiplantibacillus Plantarum Bacteriophage P2

Phage P2 was isolated from failed fermentation broth carried out by Lactiplantibacillus plantarum IMAU10120. A previous study in our laboratory showed that this phage belonged to the Siphoviridae family. In this study, this phage’s genomic characteristics were analyzed using whole-genome sequencing....

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Detalles Bibliográficos
Autores principales: Zhu, Hanfang, Guo, She, Zhao, Jie, Arbab Sakandar, Hafiz, Lv, Ruirui, Wen, Qiannan, Chen, Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2022
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608156/
https://www.ncbi.nlm.nih.gov/pubmed/36185020
http://dx.doi.org/10.33073/pjm-2022-037
Descripción
Sumario:Phage P2 was isolated from failed fermentation broth carried out by Lactiplantibacillus plantarum IMAU10120. A previous study in our laboratory showed that this phage belonged to the Siphoviridae family. In this study, this phage’s genomic characteristics were analyzed using whole-genome sequencing. It was revealed that phage P2 was 77.9 kb in length and had 39.28% G + C content. Its genome included 96 coding sequences (CDS) and two tRNA genes involved in the function of the structure, DNA replication, packaging, and regulation. Phage P2 had higher host specificity; many tested strains were not infected. Cell wall adsorption experiments showed that the adsorption receptor component of phage P2 might be a part of the cell wall peptidoglycan. This research might enrich the knowledge about genomic information of lactobacillus phages and provide some primary data to establish phage control measures.

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