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Contribution of Serum Cytomegalovirus PCR to Diagnosis of Early CMV Primary Infection in Pregnant Women
(1) Background: What is the role of serum CMV PCR in the diagnosis of recent primary infection (PI) in pregnant women when IgG avidity is uninformative? (2) Methods: Retrospective cohort study to compare serum versus whole blood CMV PCR. (a) Qualitative assessment: CMV PCR was performed on 123 serum...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608498/ https://www.ncbi.nlm.nih.gov/pubmed/36298692 http://dx.doi.org/10.3390/v14102137 |
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author | Périllaud-Dubois, Claire Bouthry, Elise Mouna, Lina Pirin, Christine Vieux-Combe, Corinne Picone, Olivier Roque-Afonso, Anne-Marie Vivanti, Alexandre J. Vauloup-Fellous, Christelle |
author_facet | Périllaud-Dubois, Claire Bouthry, Elise Mouna, Lina Pirin, Christine Vieux-Combe, Corinne Picone, Olivier Roque-Afonso, Anne-Marie Vivanti, Alexandre J. Vauloup-Fellous, Christelle |
author_sort | Périllaud-Dubois, Claire |
collection | PubMed |
description | (1) Background: What is the role of serum CMV PCR in the diagnosis of recent primary infection (PI) in pregnant women when IgG avidity is uninformative? (2) Methods: Retrospective cohort study to compare serum versus whole blood CMV PCR. (a) Qualitative assessment: CMV PCR was performed on 123 serum samples and 74 whole blood samples collected from 132 pregnant women with recent CMV PI. PCR positivity rate was used to calculate sensitivity in serum and whole blood. (b) Quantitative assessment: CMV PCR was performed on 72 paired samples of serum and whole blood collected on the same day from 57 patients. (3) Results: In pregnant women, PCR positivity rate was 89% for serum samples versus 100% in whole blood in the case of very recent PI (<15 days), but only 27% in serum versus 68% in whole blood for PI occurring from 6 weeks to 3 months before. Comparing CMV viral loads between serum and whole blood, we determined the limit of CMV DNA detection in serum as 3 log copies/mL (whole blood equivalent). (4) Conclusions: Serum CMV PCR is reliable in confirming PI in cases when only IgM is detected. It is therefore a valuable tool in introducing valaciclovir treatment as early as possible to prevent mother-to-child CMV transmission. |
format | Online Article Text |
id | pubmed-9608498 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96084982022-10-28 Contribution of Serum Cytomegalovirus PCR to Diagnosis of Early CMV Primary Infection in Pregnant Women Périllaud-Dubois, Claire Bouthry, Elise Mouna, Lina Pirin, Christine Vieux-Combe, Corinne Picone, Olivier Roque-Afonso, Anne-Marie Vivanti, Alexandre J. Vauloup-Fellous, Christelle Viruses Article (1) Background: What is the role of serum CMV PCR in the diagnosis of recent primary infection (PI) in pregnant women when IgG avidity is uninformative? (2) Methods: Retrospective cohort study to compare serum versus whole blood CMV PCR. (a) Qualitative assessment: CMV PCR was performed on 123 serum samples and 74 whole blood samples collected from 132 pregnant women with recent CMV PI. PCR positivity rate was used to calculate sensitivity in serum and whole blood. (b) Quantitative assessment: CMV PCR was performed on 72 paired samples of serum and whole blood collected on the same day from 57 patients. (3) Results: In pregnant women, PCR positivity rate was 89% for serum samples versus 100% in whole blood in the case of very recent PI (<15 days), but only 27% in serum versus 68% in whole blood for PI occurring from 6 weeks to 3 months before. Comparing CMV viral loads between serum and whole blood, we determined the limit of CMV DNA detection in serum as 3 log copies/mL (whole blood equivalent). (4) Conclusions: Serum CMV PCR is reliable in confirming PI in cases when only IgM is detected. It is therefore a valuable tool in introducing valaciclovir treatment as early as possible to prevent mother-to-child CMV transmission. MDPI 2022-09-28 /pmc/articles/PMC9608498/ /pubmed/36298692 http://dx.doi.org/10.3390/v14102137 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Périllaud-Dubois, Claire Bouthry, Elise Mouna, Lina Pirin, Christine Vieux-Combe, Corinne Picone, Olivier Roque-Afonso, Anne-Marie Vivanti, Alexandre J. Vauloup-Fellous, Christelle Contribution of Serum Cytomegalovirus PCR to Diagnosis of Early CMV Primary Infection in Pregnant Women |
title | Contribution of Serum Cytomegalovirus PCR to Diagnosis of Early CMV Primary Infection in Pregnant Women |
title_full | Contribution of Serum Cytomegalovirus PCR to Diagnosis of Early CMV Primary Infection in Pregnant Women |
title_fullStr | Contribution of Serum Cytomegalovirus PCR to Diagnosis of Early CMV Primary Infection in Pregnant Women |
title_full_unstemmed | Contribution of Serum Cytomegalovirus PCR to Diagnosis of Early CMV Primary Infection in Pregnant Women |
title_short | Contribution of Serum Cytomegalovirus PCR to Diagnosis of Early CMV Primary Infection in Pregnant Women |
title_sort | contribution of serum cytomegalovirus pcr to diagnosis of early cmv primary infection in pregnant women |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608498/ https://www.ncbi.nlm.nih.gov/pubmed/36298692 http://dx.doi.org/10.3390/v14102137 |
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