Cargando…

Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization

Ulcerative colitis (UC) is an inflammatory bowel disease caused by mucosal immune system disorder, which has increased steadily all over the world. Previous studies have shown that collagen peptide (CP) has various beneficial biological activities, it is not clear whether the effect of CP on UC is p...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xiaoxia, Cui, Luwen, Feng, Guilin, Yu, Shengnan, Shao, Guanglong, He, Ningning, Li, Shangyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608506/
https://www.ncbi.nlm.nih.gov/pubmed/36313077
http://dx.doi.org/10.3389/fnut.2022.957391
_version_ 1784818788595662848
author Li, Xiaoxia
Cui, Luwen
Feng, Guilin
Yu, Shengnan
Shao, Guanglong
He, Ningning
Li, Shangyong
author_facet Li, Xiaoxia
Cui, Luwen
Feng, Guilin
Yu, Shengnan
Shao, Guanglong
He, Ningning
Li, Shangyong
author_sort Li, Xiaoxia
collection PubMed
description Ulcerative colitis (UC) is an inflammatory bowel disease caused by mucosal immune system disorder, which has increased steadily all over the world. Previous studies have shown that collagen peptide (CP) has various beneficial biological activities, it is not clear whether the effect of CP on UC is positive or negative. In this study, 2.5% dextran sulfate sodium (DSS) was used to establish acute colitis in mice. Our results suggested that CP supplementation (200, 400 mg/kg/day) promoted the progression of colitis, increased the expression of inflammatory factors and the infiltration of colonic lamina propria macrophages. Gut microbiota analysis showed the composition changed significantly and inflammation promoted bacteria was after CP treatment. Meanwhile, the effect of CP on macrophage polarization was further determined in Raw264.7 cell line. The results showed that CP treatment could increase the polarization of M1 macrophages and promote the expression of inflammatory factors. In conclusion, our results showed that CP treatment could disrupt the gut microbiota of host, promote macrophage activation and aggravate DSS-induced colitis. This may suggest that patients with intestinal inflammation should not take marine derived CP.
format Online
Article
Text
id pubmed-9608506
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-96085062022-10-28 Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization Li, Xiaoxia Cui, Luwen Feng, Guilin Yu, Shengnan Shao, Guanglong He, Ningning Li, Shangyong Front Nutr Nutrition Ulcerative colitis (UC) is an inflammatory bowel disease caused by mucosal immune system disorder, which has increased steadily all over the world. Previous studies have shown that collagen peptide (CP) has various beneficial biological activities, it is not clear whether the effect of CP on UC is positive or negative. In this study, 2.5% dextran sulfate sodium (DSS) was used to establish acute colitis in mice. Our results suggested that CP supplementation (200, 400 mg/kg/day) promoted the progression of colitis, increased the expression of inflammatory factors and the infiltration of colonic lamina propria macrophages. Gut microbiota analysis showed the composition changed significantly and inflammation promoted bacteria was after CP treatment. Meanwhile, the effect of CP on macrophage polarization was further determined in Raw264.7 cell line. The results showed that CP treatment could increase the polarization of M1 macrophages and promote the expression of inflammatory factors. In conclusion, our results showed that CP treatment could disrupt the gut microbiota of host, promote macrophage activation and aggravate DSS-induced colitis. This may suggest that patients with intestinal inflammation should not take marine derived CP. Frontiers Media S.A. 2022-10-13 /pmc/articles/PMC9608506/ /pubmed/36313077 http://dx.doi.org/10.3389/fnut.2022.957391 Text en Copyright © 2022 Li, Cui, Feng, Yu, Shao, He and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Li, Xiaoxia
Cui, Luwen
Feng, Guilin
Yu, Shengnan
Shao, Guanglong
He, Ningning
Li, Shangyong
Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization
title Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization
title_full Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization
title_fullStr Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization
title_full_unstemmed Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization
title_short Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization
title_sort collagen peptide promotes dss-induced colitis by disturbing gut microbiota and regulation of macrophage polarization
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608506/
https://www.ncbi.nlm.nih.gov/pubmed/36313077
http://dx.doi.org/10.3389/fnut.2022.957391
work_keys_str_mv AT lixiaoxia collagenpeptidepromotesdssinducedcolitisbydisturbinggutmicrobiotaandregulationofmacrophagepolarization
AT cuiluwen collagenpeptidepromotesdssinducedcolitisbydisturbinggutmicrobiotaandregulationofmacrophagepolarization
AT fengguilin collagenpeptidepromotesdssinducedcolitisbydisturbinggutmicrobiotaandregulationofmacrophagepolarization
AT yushengnan collagenpeptidepromotesdssinducedcolitisbydisturbinggutmicrobiotaandregulationofmacrophagepolarization
AT shaoguanglong collagenpeptidepromotesdssinducedcolitisbydisturbinggutmicrobiotaandregulationofmacrophagepolarization
AT heningning collagenpeptidepromotesdssinducedcolitisbydisturbinggutmicrobiotaandregulationofmacrophagepolarization
AT lishangyong collagenpeptidepromotesdssinducedcolitisbydisturbinggutmicrobiotaandregulationofmacrophagepolarization