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A monoamine oxidase B inhibitor ethyl ferulate suppresses microglia-mediated neuroinflammation and alleviates ischemic brain injury
Microglia are the resident macrophages in the brain, which play a critical role in post-stroke neuroinflammation. Accordingly, targeting neuroinflammation could be a promising strategy to improve ischemic stroke outcomes. Ethyl ferulate (EF) has been confirmed to possess anti-inflammatory properties...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608666/ https://www.ncbi.nlm.nih.gov/pubmed/36313349 http://dx.doi.org/10.3389/fphar.2022.1004215 |
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author | Zou, Xinxin Gao, Shenghan Li, Jiangnan Li, Chenggang Wu, Chuyu Cao, Xiang Xia, Shengnan Shao, Pengfei Bao, Xinyu Yang, Haiyan Liu, Pinyi Xu, Yun |
author_facet | Zou, Xinxin Gao, Shenghan Li, Jiangnan Li, Chenggang Wu, Chuyu Cao, Xiang Xia, Shengnan Shao, Pengfei Bao, Xinyu Yang, Haiyan Liu, Pinyi Xu, Yun |
author_sort | Zou, Xinxin |
collection | PubMed |
description | Microglia are the resident macrophages in the brain, which play a critical role in post-stroke neuroinflammation. Accordingly, targeting neuroinflammation could be a promising strategy to improve ischemic stroke outcomes. Ethyl ferulate (EF) has been confirmed to possess anti-inflammatory properties in several disease models, including acute lung injury, retinal damage and diabetes-associated renal injury. However, the effects of EF on microglial activation and the resolution of post-stroke neuroinflammation remains unknown. Here, we found that EF suppressed pro-inflammatory response triggered by lipopolysaccharide (LPS) stimulation in primary microglia and BV2 cell lines, as well as post-stroke neuroinflammation in an in vivo transient middle cerebral artery occlusion (tMCAO) stroke model in C57BL/6 mice, consequently ameliorating ischemic brain injury. Furthermore, EF could directly bind and inhibit the activity of monoamine oxidase B (MAO-B) to reduce pro-inflammatory response. Taken together, our study identified a MAO-B inhibitor, Ethyl ferulate, as an active compound with promising potentials for suppressing post-stroke neuroinflammation. |
format | Online Article Text |
id | pubmed-9608666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96086662022-10-28 A monoamine oxidase B inhibitor ethyl ferulate suppresses microglia-mediated neuroinflammation and alleviates ischemic brain injury Zou, Xinxin Gao, Shenghan Li, Jiangnan Li, Chenggang Wu, Chuyu Cao, Xiang Xia, Shengnan Shao, Pengfei Bao, Xinyu Yang, Haiyan Liu, Pinyi Xu, Yun Front Pharmacol Pharmacology Microglia are the resident macrophages in the brain, which play a critical role in post-stroke neuroinflammation. Accordingly, targeting neuroinflammation could be a promising strategy to improve ischemic stroke outcomes. Ethyl ferulate (EF) has been confirmed to possess anti-inflammatory properties in several disease models, including acute lung injury, retinal damage and diabetes-associated renal injury. However, the effects of EF on microglial activation and the resolution of post-stroke neuroinflammation remains unknown. Here, we found that EF suppressed pro-inflammatory response triggered by lipopolysaccharide (LPS) stimulation in primary microglia and BV2 cell lines, as well as post-stroke neuroinflammation in an in vivo transient middle cerebral artery occlusion (tMCAO) stroke model in C57BL/6 mice, consequently ameliorating ischemic brain injury. Furthermore, EF could directly bind and inhibit the activity of monoamine oxidase B (MAO-B) to reduce pro-inflammatory response. Taken together, our study identified a MAO-B inhibitor, Ethyl ferulate, as an active compound with promising potentials for suppressing post-stroke neuroinflammation. Frontiers Media S.A. 2022-10-13 /pmc/articles/PMC9608666/ /pubmed/36313349 http://dx.doi.org/10.3389/fphar.2022.1004215 Text en Copyright © 2022 Zou, Gao, Li, Li, Wu, Cao, Xia, Shao, Bao, Yang, Liu and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Zou, Xinxin Gao, Shenghan Li, Jiangnan Li, Chenggang Wu, Chuyu Cao, Xiang Xia, Shengnan Shao, Pengfei Bao, Xinyu Yang, Haiyan Liu, Pinyi Xu, Yun A monoamine oxidase B inhibitor ethyl ferulate suppresses microglia-mediated neuroinflammation and alleviates ischemic brain injury |
title | A monoamine oxidase B inhibitor ethyl ferulate suppresses microglia-mediated neuroinflammation and alleviates ischemic brain injury |
title_full | A monoamine oxidase B inhibitor ethyl ferulate suppresses microglia-mediated neuroinflammation and alleviates ischemic brain injury |
title_fullStr | A monoamine oxidase B inhibitor ethyl ferulate suppresses microglia-mediated neuroinflammation and alleviates ischemic brain injury |
title_full_unstemmed | A monoamine oxidase B inhibitor ethyl ferulate suppresses microglia-mediated neuroinflammation and alleviates ischemic brain injury |
title_short | A monoamine oxidase B inhibitor ethyl ferulate suppresses microglia-mediated neuroinflammation and alleviates ischemic brain injury |
title_sort | monoamine oxidase b inhibitor ethyl ferulate suppresses microglia-mediated neuroinflammation and alleviates ischemic brain injury |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608666/ https://www.ncbi.nlm.nih.gov/pubmed/36313349 http://dx.doi.org/10.3389/fphar.2022.1004215 |
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