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Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging

The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, a...

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Autores principales: Yang, Junhai, Rendino, Lauren, Cassar, Steven, Buck, Wayne, Sawicki, James, Talaty, Nari, Wagner, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608760/
https://www.ncbi.nlm.nih.gov/pubmed/36297342
http://dx.doi.org/10.3390/ph15101230
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author Yang, Junhai
Rendino, Lauren
Cassar, Steven
Buck, Wayne
Sawicki, James
Talaty, Nari
Wagner, David
author_facet Yang, Junhai
Rendino, Lauren
Cassar, Steven
Buck, Wayne
Sawicki, James
Talaty, Nari
Wagner, David
author_sort Yang, Junhai
collection PubMed
description The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, and locations for comparison purposes is a challenging task. We report here the optimization of fresh frozen zebrafish larvae sectioning for mass spectrometry imaging. We utilized the gelatin solutions that were created at two different temperatures (50 and 85 °C) as embedding media. Gelatin-50 (gelatin created under 50 °C, solid gel under room temperature) was used to make a larvae-shaped mold and gelatin-85 (gelatin created under 85 °C, liquid under room temperature) was used to embed the larvae. H&E staining of sections shows well-preserved morphology and minimal histological interference. More importantly, the position of the larvae was well controlled resulting in more consistent sectioning of the larvae.
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spelling pubmed-96087602022-10-28 Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging Yang, Junhai Rendino, Lauren Cassar, Steven Buck, Wayne Sawicki, James Talaty, Nari Wagner, David Pharmaceuticals (Basel) Article The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, and locations for comparison purposes is a challenging task. We report here the optimization of fresh frozen zebrafish larvae sectioning for mass spectrometry imaging. We utilized the gelatin solutions that were created at two different temperatures (50 and 85 °C) as embedding media. Gelatin-50 (gelatin created under 50 °C, solid gel under room temperature) was used to make a larvae-shaped mold and gelatin-85 (gelatin created under 85 °C, liquid under room temperature) was used to embed the larvae. H&E staining of sections shows well-preserved morphology and minimal histological interference. More importantly, the position of the larvae was well controlled resulting in more consistent sectioning of the larvae. MDPI 2022-10-07 /pmc/articles/PMC9608760/ /pubmed/36297342 http://dx.doi.org/10.3390/ph15101230 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Junhai
Rendino, Lauren
Cassar, Steven
Buck, Wayne
Sawicki, James
Talaty, Nari
Wagner, David
Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging
title Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging
title_full Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging
title_fullStr Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging
title_full_unstemmed Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging
title_short Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging
title_sort optimization of zebrafish larvae sectioning for mass spectrometry imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608760/
https://www.ncbi.nlm.nih.gov/pubmed/36297342
http://dx.doi.org/10.3390/ph15101230
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