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Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging
The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608760/ https://www.ncbi.nlm.nih.gov/pubmed/36297342 http://dx.doi.org/10.3390/ph15101230 |
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author | Yang, Junhai Rendino, Lauren Cassar, Steven Buck, Wayne Sawicki, James Talaty, Nari Wagner, David |
author_facet | Yang, Junhai Rendino, Lauren Cassar, Steven Buck, Wayne Sawicki, James Talaty, Nari Wagner, David |
author_sort | Yang, Junhai |
collection | PubMed |
description | The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, and locations for comparison purposes is a challenging task. We report here the optimization of fresh frozen zebrafish larvae sectioning for mass spectrometry imaging. We utilized the gelatin solutions that were created at two different temperatures (50 and 85 °C) as embedding media. Gelatin-50 (gelatin created under 50 °C, solid gel under room temperature) was used to make a larvae-shaped mold and gelatin-85 (gelatin created under 85 °C, liquid under room temperature) was used to embed the larvae. H&E staining of sections shows well-preserved morphology and minimal histological interference. More importantly, the position of the larvae was well controlled resulting in more consistent sectioning of the larvae. |
format | Online Article Text |
id | pubmed-9608760 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96087602022-10-28 Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging Yang, Junhai Rendino, Lauren Cassar, Steven Buck, Wayne Sawicki, James Talaty, Nari Wagner, David Pharmaceuticals (Basel) Article The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, and locations for comparison purposes is a challenging task. We report here the optimization of fresh frozen zebrafish larvae sectioning for mass spectrometry imaging. We utilized the gelatin solutions that were created at two different temperatures (50 and 85 °C) as embedding media. Gelatin-50 (gelatin created under 50 °C, solid gel under room temperature) was used to make a larvae-shaped mold and gelatin-85 (gelatin created under 85 °C, liquid under room temperature) was used to embed the larvae. H&E staining of sections shows well-preserved morphology and minimal histological interference. More importantly, the position of the larvae was well controlled resulting in more consistent sectioning of the larvae. MDPI 2022-10-07 /pmc/articles/PMC9608760/ /pubmed/36297342 http://dx.doi.org/10.3390/ph15101230 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Junhai Rendino, Lauren Cassar, Steven Buck, Wayne Sawicki, James Talaty, Nari Wagner, David Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging |
title | Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging |
title_full | Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging |
title_fullStr | Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging |
title_full_unstemmed | Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging |
title_short | Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging |
title_sort | optimization of zebrafish larvae sectioning for mass spectrometry imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608760/ https://www.ncbi.nlm.nih.gov/pubmed/36297342 http://dx.doi.org/10.3390/ph15101230 |
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