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Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs
Butyrate, a by-product of gut bacteria fermentation as well as the digestion of fat in mother’s milk, exerts a wide spectrum of beneficial effects in the gastrointestinal tissues. The present study aimed to determine the effects of sodium butyrate on small intestine contractility in neonatal piglets...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608939/ https://www.ncbi.nlm.nih.gov/pubmed/36296985 http://dx.doi.org/10.3390/nu14204301 |
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author | Słupecka-Ziemilska, Monika Pierzynowski, Stefan Grzegorz Szczurek, Paulina Pierzynowska, Kateryna Wychowański, Piotr Seklecka, Blanka Koperski, Maciej Starzyńska, Anna Szkopek, Dominika Donaldson, Janine Andrzejewski, Krzysztof Woliński, Jarosław |
author_facet | Słupecka-Ziemilska, Monika Pierzynowski, Stefan Grzegorz Szczurek, Paulina Pierzynowska, Kateryna Wychowański, Piotr Seklecka, Blanka Koperski, Maciej Starzyńska, Anna Szkopek, Dominika Donaldson, Janine Andrzejewski, Krzysztof Woliński, Jarosław |
author_sort | Słupecka-Ziemilska, Monika |
collection | PubMed |
description | Butyrate, a by-product of gut bacteria fermentation as well as the digestion of fat in mother’s milk, exerts a wide spectrum of beneficial effects in the gastrointestinal tissues. The present study aimed to determine the effects of sodium butyrate on small intestine contractility in neonatal piglets. Piglets were fed milk formula alone (group C) or milk formula supplemented with sodium butyrate (group B). After a 7-day treatment period, isometric recordings of whole-thickness segments of the duodenum and middle jejunum were obtained by electric field stimulation under the influence of increasing doses of Ach (acetylocholine) in the presence of TTX (tetrodotoxin) and atropine. Moreover, structural properties of the intestinal wall were assessed, together with the expression of cholinergic and muscarinic receptors (M1 and M2). In both intestinal segments (duodenum and middle jejunum), EFS (electric field stimulation) impulses resulted in increased contractility and amplitude of contractions in group B compared to group C. Additionally, exposure to dietary butyrate led to a significant increase in tunica muscularis thickness in the duodenum, while mitotic and apoptotic indices were increased in the middle jejunum. The expression of M1 and M2 receptors in the middle jejunum was significantly higher after butyrate treatment. The results indicate increased cholinergic signaling and small intestinal growth and renewal in response to feeding with milk formula enriched with sodium butyrate in neonatal piglets. |
format | Online Article Text |
id | pubmed-9608939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96089392022-10-28 Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs Słupecka-Ziemilska, Monika Pierzynowski, Stefan Grzegorz Szczurek, Paulina Pierzynowska, Kateryna Wychowański, Piotr Seklecka, Blanka Koperski, Maciej Starzyńska, Anna Szkopek, Dominika Donaldson, Janine Andrzejewski, Krzysztof Woliński, Jarosław Nutrients Article Butyrate, a by-product of gut bacteria fermentation as well as the digestion of fat in mother’s milk, exerts a wide spectrum of beneficial effects in the gastrointestinal tissues. The present study aimed to determine the effects of sodium butyrate on small intestine contractility in neonatal piglets. Piglets were fed milk formula alone (group C) or milk formula supplemented with sodium butyrate (group B). After a 7-day treatment period, isometric recordings of whole-thickness segments of the duodenum and middle jejunum were obtained by electric field stimulation under the influence of increasing doses of Ach (acetylocholine) in the presence of TTX (tetrodotoxin) and atropine. Moreover, structural properties of the intestinal wall were assessed, together with the expression of cholinergic and muscarinic receptors (M1 and M2). In both intestinal segments (duodenum and middle jejunum), EFS (electric field stimulation) impulses resulted in increased contractility and amplitude of contractions in group B compared to group C. Additionally, exposure to dietary butyrate led to a significant increase in tunica muscularis thickness in the duodenum, while mitotic and apoptotic indices were increased in the middle jejunum. The expression of M1 and M2 receptors in the middle jejunum was significantly higher after butyrate treatment. The results indicate increased cholinergic signaling and small intestinal growth and renewal in response to feeding with milk formula enriched with sodium butyrate in neonatal piglets. MDPI 2022-10-14 /pmc/articles/PMC9608939/ /pubmed/36296985 http://dx.doi.org/10.3390/nu14204301 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Słupecka-Ziemilska, Monika Pierzynowski, Stefan Grzegorz Szczurek, Paulina Pierzynowska, Kateryna Wychowański, Piotr Seklecka, Blanka Koperski, Maciej Starzyńska, Anna Szkopek, Dominika Donaldson, Janine Andrzejewski, Krzysztof Woliński, Jarosław Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs |
title | Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs |
title_full | Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs |
title_fullStr | Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs |
title_full_unstemmed | Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs |
title_short | Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs |
title_sort | milk formula enriched with sodium butyrate influences small intestine contractility in neonatal pigs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608939/ https://www.ncbi.nlm.nih.gov/pubmed/36296985 http://dx.doi.org/10.3390/nu14204301 |
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