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Adaptive Feature Extraction for Blood Vessel Segmentation and Contrast Recalculation in Laser Speckle Contrast Imaging

Microvasculature analysis in biomedical images is essential in the medical area to evaluate diseases by extracting properties of blood vessels, such as relative blood flow or morphological measurements such as diameter. Given the advantages of Laser Speckle Contrast Imaging (LSCI), several studies h...

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Autores principales: Morales-Vargas, Eduardo, Padilla-Martinez, Juan Pablo, Peregrina-Barreto, Hayde, Garcia-Suastegui, Wendy Argelia, Ramirez-San-Juan, Julio Cesar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609016/
https://www.ncbi.nlm.nih.gov/pubmed/36296143
http://dx.doi.org/10.3390/mi13101788
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author Morales-Vargas, Eduardo
Padilla-Martinez, Juan Pablo
Peregrina-Barreto, Hayde
Garcia-Suastegui, Wendy Argelia
Ramirez-San-Juan, Julio Cesar
author_facet Morales-Vargas, Eduardo
Padilla-Martinez, Juan Pablo
Peregrina-Barreto, Hayde
Garcia-Suastegui, Wendy Argelia
Ramirez-San-Juan, Julio Cesar
author_sort Morales-Vargas, Eduardo
collection PubMed
description Microvasculature analysis in biomedical images is essential in the medical area to evaluate diseases by extracting properties of blood vessels, such as relative blood flow or morphological measurements such as diameter. Given the advantages of Laser Speckle Contrast Imaging (LSCI), several studies have aimed to reduce inherent noise to distinguish between tissue and blood vessels at higher depths. These studies have shown that computing Contrast Images (CIs) with Analysis Windows (AWs) larger than standard sizes obtains better statistical estimators. The main issue is that larger samples combine pixels of microvasculature with tissue regions, reducing the spatial resolution of the CI. This work proposes using adaptive AWs of variable size and shape to calculate the features required to train a segmentation model that discriminates between blood vessels and tissue in LSCI. The obtained results show that it is possible to improve segmentation rates of blood vessels up to 45% in high depths (≈900 [Formula: see text]) by extracting features adaptively. The main contribution of this work is the experimentation with LSCI images under different depths and exposure times through adaptive processing methods, furthering the understanding the performance of the different approaches under these conditions. Results also suggest that it is possible to train a segmentation model to discriminate between pixels belonging to blood vessels and those belonging to tissue. Therefore, an adaptive feature extraction method may improve the quality of the features and thus increase the classification rates of blood vessels in LSCI.
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spelling pubmed-96090162022-10-28 Adaptive Feature Extraction for Blood Vessel Segmentation and Contrast Recalculation in Laser Speckle Contrast Imaging Morales-Vargas, Eduardo Padilla-Martinez, Juan Pablo Peregrina-Barreto, Hayde Garcia-Suastegui, Wendy Argelia Ramirez-San-Juan, Julio Cesar Micromachines (Basel) Article Microvasculature analysis in biomedical images is essential in the medical area to evaluate diseases by extracting properties of blood vessels, such as relative blood flow or morphological measurements such as diameter. Given the advantages of Laser Speckle Contrast Imaging (LSCI), several studies have aimed to reduce inherent noise to distinguish between tissue and blood vessels at higher depths. These studies have shown that computing Contrast Images (CIs) with Analysis Windows (AWs) larger than standard sizes obtains better statistical estimators. The main issue is that larger samples combine pixels of microvasculature with tissue regions, reducing the spatial resolution of the CI. This work proposes using adaptive AWs of variable size and shape to calculate the features required to train a segmentation model that discriminates between blood vessels and tissue in LSCI. The obtained results show that it is possible to improve segmentation rates of blood vessels up to 45% in high depths (≈900 [Formula: see text]) by extracting features adaptively. The main contribution of this work is the experimentation with LSCI images under different depths and exposure times through adaptive processing methods, furthering the understanding the performance of the different approaches under these conditions. Results also suggest that it is possible to train a segmentation model to discriminate between pixels belonging to blood vessels and those belonging to tissue. Therefore, an adaptive feature extraction method may improve the quality of the features and thus increase the classification rates of blood vessels in LSCI. MDPI 2022-10-20 /pmc/articles/PMC9609016/ /pubmed/36296143 http://dx.doi.org/10.3390/mi13101788 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Morales-Vargas, Eduardo
Padilla-Martinez, Juan Pablo
Peregrina-Barreto, Hayde
Garcia-Suastegui, Wendy Argelia
Ramirez-San-Juan, Julio Cesar
Adaptive Feature Extraction for Blood Vessel Segmentation and Contrast Recalculation in Laser Speckle Contrast Imaging
title Adaptive Feature Extraction for Blood Vessel Segmentation and Contrast Recalculation in Laser Speckle Contrast Imaging
title_full Adaptive Feature Extraction for Blood Vessel Segmentation and Contrast Recalculation in Laser Speckle Contrast Imaging
title_fullStr Adaptive Feature Extraction for Blood Vessel Segmentation and Contrast Recalculation in Laser Speckle Contrast Imaging
title_full_unstemmed Adaptive Feature Extraction for Blood Vessel Segmentation and Contrast Recalculation in Laser Speckle Contrast Imaging
title_short Adaptive Feature Extraction for Blood Vessel Segmentation and Contrast Recalculation in Laser Speckle Contrast Imaging
title_sort adaptive feature extraction for blood vessel segmentation and contrast recalculation in laser speckle contrast imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609016/
https://www.ncbi.nlm.nih.gov/pubmed/36296143
http://dx.doi.org/10.3390/mi13101788
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