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Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span

Impedance cytometry is wildly used in single-cell detection, and its sensitivity is essential for determining the status of single cells. In this work, we focus on the effect of electrode gap on detection sensitivity. Through comparing the electrode span of 1 µm and 5 µm, our work shows that narrowi...

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Autores principales: Liu, Xun, Tang, Tao, Yi, Po-Wei, Yuan, Yapeng, Lei, Cheng, Li, Ming, Tanaka, Yo, Hosokawa, Yoichiroh, Yalikun, Yaxiaer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609181/
https://www.ncbi.nlm.nih.gov/pubmed/36298094
http://dx.doi.org/10.3390/s22207743
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author Liu, Xun
Tang, Tao
Yi, Po-Wei
Yuan, Yapeng
Lei, Cheng
Li, Ming
Tanaka, Yo
Hosokawa, Yoichiroh
Yalikun, Yaxiaer
author_facet Liu, Xun
Tang, Tao
Yi, Po-Wei
Yuan, Yapeng
Lei, Cheng
Li, Ming
Tanaka, Yo
Hosokawa, Yoichiroh
Yalikun, Yaxiaer
author_sort Liu, Xun
collection PubMed
description Impedance cytometry is wildly used in single-cell detection, and its sensitivity is essential for determining the status of single cells. In this work, we focus on the effect of electrode gap on detection sensitivity. Through comparing the electrode span of 1 µm and 5 µm, our work shows that narrowing the electrode span could greatly improve detection sensitivity. The mechanism underlying the sensitivity improvement was analyzed via numerical simulation. The small electrode gap (1 µm) allows the electric field to concentrate near the detection area, resulting in a high sensitivity for tiny particles. This finding is also verified with the mixture suspension of 1 µm and 3 µm polystyrene beads. As a result, the electrodes with 1 µm gap can detect more 1 µm beads in the suspension than electrodes with 5 µm gap. Additionally, for single yeast cells analysis, it is found that impedance cytometry with 1 µm electrodes gap can easily distinguish budding yeast cells, which cannot be realized by the impedance cytometry with 5 µm electrodes gap. All experimental results support that narrowing the electrode gap is necessary for tiny particle detection, which is an important step in the development of submicron and nanoscale impedance cytometry.
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spelling pubmed-96091812022-10-28 Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span Liu, Xun Tang, Tao Yi, Po-Wei Yuan, Yapeng Lei, Cheng Li, Ming Tanaka, Yo Hosokawa, Yoichiroh Yalikun, Yaxiaer Sensors (Basel) Article Impedance cytometry is wildly used in single-cell detection, and its sensitivity is essential for determining the status of single cells. In this work, we focus on the effect of electrode gap on detection sensitivity. Through comparing the electrode span of 1 µm and 5 µm, our work shows that narrowing the electrode span could greatly improve detection sensitivity. The mechanism underlying the sensitivity improvement was analyzed via numerical simulation. The small electrode gap (1 µm) allows the electric field to concentrate near the detection area, resulting in a high sensitivity for tiny particles. This finding is also verified with the mixture suspension of 1 µm and 3 µm polystyrene beads. As a result, the electrodes with 1 µm gap can detect more 1 µm beads in the suspension than electrodes with 5 µm gap. Additionally, for single yeast cells analysis, it is found that impedance cytometry with 1 µm electrodes gap can easily distinguish budding yeast cells, which cannot be realized by the impedance cytometry with 5 µm electrodes gap. All experimental results support that narrowing the electrode gap is necessary for tiny particle detection, which is an important step in the development of submicron and nanoscale impedance cytometry. MDPI 2022-10-12 /pmc/articles/PMC9609181/ /pubmed/36298094 http://dx.doi.org/10.3390/s22207743 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xun
Tang, Tao
Yi, Po-Wei
Yuan, Yapeng
Lei, Cheng
Li, Ming
Tanaka, Yo
Hosokawa, Yoichiroh
Yalikun, Yaxiaer
Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span
title Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span
title_full Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span
title_fullStr Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span
title_full_unstemmed Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span
title_short Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span
title_sort identification of single yeast budding using impedance cytometry with a narrow electrode span
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609181/
https://www.ncbi.nlm.nih.gov/pubmed/36298094
http://dx.doi.org/10.3390/s22207743
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