Cargando…

Environmental Adaptability and Organic Pollutant Degradation Capacity of a Novel Rhodococcus Species Derived from Soil in the Uninhabited Area of the Qinghai-Tibet Plateau

The Qinghai-Tibet Plateau (QTP) is known for extreme natural environments and, surprisingly, has been reported to contain widespread organic pollutants. Rhodococcus can survive a variety of extreme environments and degrade many organic contaminants. Here, we isolated a Rhodococcus strain (FXJ9.536 =...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Jiao, Ai, Guomin, Liu, Ning, Huang, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609184/
https://www.ncbi.nlm.nih.gov/pubmed/36296211
http://dx.doi.org/10.3390/microorganisms10101935
_version_ 1784818957102874624
author Huang, Jiao
Ai, Guomin
Liu, Ning
Huang, Ying
author_facet Huang, Jiao
Ai, Guomin
Liu, Ning
Huang, Ying
author_sort Huang, Jiao
collection PubMed
description The Qinghai-Tibet Plateau (QTP) is known for extreme natural environments and, surprisingly, has been reported to contain widespread organic pollutants. Rhodococcus can survive a variety of extreme environments and degrade many organic contaminants. Here, we isolated a Rhodococcus strain (FXJ9.536 = CGMCC 4.7853) from a soil sample collected in the QTP. Phylogenomic analysis indicated that the strain represents a novel Rhodococcus species, for which the name Rhodococcus tibetensis sp. nov. is proposed. Interestingly, R. tibetensis FXJ9.536 maintained a fast growth rate and degraded 6.2% of p-nitrophenol (4-NP) and 50.0% of malathion even at 10 °C. It could degrade 53.6% of 4-NP and 99.9% of malathion at a moderate temperature. The genome of R. tibetensis FXJ9.536 contains 4-hydroxyphenylacetate 3-monoxygenase and carboxylesterase genes, which are likely associated with the degradation of 4-NP and malathion, respectively. Further genomic analysis revealed that the strain might employ multiple strategies to adapt to the harsh QTP environment. These include synthesizing cold shock proteins, compatible solutes, secondary metabolites, and storage compounds, utilizing inorganic compounds as energy and nutrition sources, as well as degrading a range of organic pollutants. Overall, our study reveals the potential of a QTP-derived new actinobacterial species for environmental adaptation and remediation in cold regions.
format Online
Article
Text
id pubmed-9609184
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96091842022-10-28 Environmental Adaptability and Organic Pollutant Degradation Capacity of a Novel Rhodococcus Species Derived from Soil in the Uninhabited Area of the Qinghai-Tibet Plateau Huang, Jiao Ai, Guomin Liu, Ning Huang, Ying Microorganisms Article The Qinghai-Tibet Plateau (QTP) is known for extreme natural environments and, surprisingly, has been reported to contain widespread organic pollutants. Rhodococcus can survive a variety of extreme environments and degrade many organic contaminants. Here, we isolated a Rhodococcus strain (FXJ9.536 = CGMCC 4.7853) from a soil sample collected in the QTP. Phylogenomic analysis indicated that the strain represents a novel Rhodococcus species, for which the name Rhodococcus tibetensis sp. nov. is proposed. Interestingly, R. tibetensis FXJ9.536 maintained a fast growth rate and degraded 6.2% of p-nitrophenol (4-NP) and 50.0% of malathion even at 10 °C. It could degrade 53.6% of 4-NP and 99.9% of malathion at a moderate temperature. The genome of R. tibetensis FXJ9.536 contains 4-hydroxyphenylacetate 3-monoxygenase and carboxylesterase genes, which are likely associated with the degradation of 4-NP and malathion, respectively. Further genomic analysis revealed that the strain might employ multiple strategies to adapt to the harsh QTP environment. These include synthesizing cold shock proteins, compatible solutes, secondary metabolites, and storage compounds, utilizing inorganic compounds as energy and nutrition sources, as well as degrading a range of organic pollutants. Overall, our study reveals the potential of a QTP-derived new actinobacterial species for environmental adaptation and remediation in cold regions. MDPI 2022-09-29 /pmc/articles/PMC9609184/ /pubmed/36296211 http://dx.doi.org/10.3390/microorganisms10101935 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Jiao
Ai, Guomin
Liu, Ning
Huang, Ying
Environmental Adaptability and Organic Pollutant Degradation Capacity of a Novel Rhodococcus Species Derived from Soil in the Uninhabited Area of the Qinghai-Tibet Plateau
title Environmental Adaptability and Organic Pollutant Degradation Capacity of a Novel Rhodococcus Species Derived from Soil in the Uninhabited Area of the Qinghai-Tibet Plateau
title_full Environmental Adaptability and Organic Pollutant Degradation Capacity of a Novel Rhodococcus Species Derived from Soil in the Uninhabited Area of the Qinghai-Tibet Plateau
title_fullStr Environmental Adaptability and Organic Pollutant Degradation Capacity of a Novel Rhodococcus Species Derived from Soil in the Uninhabited Area of the Qinghai-Tibet Plateau
title_full_unstemmed Environmental Adaptability and Organic Pollutant Degradation Capacity of a Novel Rhodococcus Species Derived from Soil in the Uninhabited Area of the Qinghai-Tibet Plateau
title_short Environmental Adaptability and Organic Pollutant Degradation Capacity of a Novel Rhodococcus Species Derived from Soil in the Uninhabited Area of the Qinghai-Tibet Plateau
title_sort environmental adaptability and organic pollutant degradation capacity of a novel rhodococcus species derived from soil in the uninhabited area of the qinghai-tibet plateau
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609184/
https://www.ncbi.nlm.nih.gov/pubmed/36296211
http://dx.doi.org/10.3390/microorganisms10101935
work_keys_str_mv AT huangjiao environmentaladaptabilityandorganicpollutantdegradationcapacityofanovelrhodococcusspeciesderivedfromsoilintheuninhabitedareaoftheqinghaitibetplateau
AT aiguomin environmentaladaptabilityandorganicpollutantdegradationcapacityofanovelrhodococcusspeciesderivedfromsoilintheuninhabitedareaoftheqinghaitibetplateau
AT liuning environmentaladaptabilityandorganicpollutantdegradationcapacityofanovelrhodococcusspeciesderivedfromsoilintheuninhabitedareaoftheqinghaitibetplateau
AT huangying environmentaladaptabilityandorganicpollutantdegradationcapacityofanovelrhodococcusspeciesderivedfromsoilintheuninhabitedareaoftheqinghaitibetplateau