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Development of a Polymeric Membrane Impregnated with Poly-Lactic Acid (PLA) Nanoparticles Loaded with Red Propolis (RP)

The main objectives of this study were to develop and characterize hydrophilic polymeric membranes impregnated with poly-lactic acid (PLA) nanoparticles (NPs) combined with red propolis (RP). Ultrasonic-assisted extraction was used to obtain 30% (w/v) red propolis hydroalcoholic extract (RPE). The N...

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Autores principales: da Costa Silva, Valdemir, do Nascimento, Ticiano G., Mergulhão, Naianny L. O. N., Freitas, Johnnatan D., Duarte, Ilza Fernanda B., de Bulhões, Laisa Carolina G., Dornelas, Camila B., de Araújo, João Xavier, dos Santos, Jucenir, Silva, Anielle C. A., Basílio, Irinaldo D., Goulart, Marilia O. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609202/
https://www.ncbi.nlm.nih.gov/pubmed/36296550
http://dx.doi.org/10.3390/molecules27206959
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author da Costa Silva, Valdemir
do Nascimento, Ticiano G.
Mergulhão, Naianny L. O. N.
Freitas, Johnnatan D.
Duarte, Ilza Fernanda B.
de Bulhões, Laisa Carolina G.
Dornelas, Camila B.
de Araújo, João Xavier
dos Santos, Jucenir
Silva, Anielle C. A.
Basílio, Irinaldo D.
Goulart, Marilia O. F.
author_facet da Costa Silva, Valdemir
do Nascimento, Ticiano G.
Mergulhão, Naianny L. O. N.
Freitas, Johnnatan D.
Duarte, Ilza Fernanda B.
de Bulhões, Laisa Carolina G.
Dornelas, Camila B.
de Araújo, João Xavier
dos Santos, Jucenir
Silva, Anielle C. A.
Basílio, Irinaldo D.
Goulart, Marilia O. F.
author_sort da Costa Silva, Valdemir
collection PubMed
description The main objectives of this study were to develop and characterize hydrophilic polymeric membranes impregnated with poly-lactic acid (PLA) nanoparticles (NPs) combined with red propolis (RP). Ultrasonic-assisted extraction was used to obtain 30% (w/v) red propolis hydroalcoholic extract (RPE). The NPs (75,000 g mol(−1)) alone and incorporated with RP (NPRP) were obtained using the solvent emulsification and diffusion technique. Biopolymeric hydrogel membranes (MNPRP) were obtained using carboxymethylcellulose (CMC) and NPRP. Their characterization was performed using thermal analysis, Fourier transform infrared (FTIR), total phenols (TPC) and flavonoids contents (TFC), and antioxidant activity through the radical scavenging assay with 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and Ferric reducing antioxidant power (FRAP). The identification and quantification of significant RP markers were performed through UPLC-DAD. The NPs were evaluated for particle size, polydispersity index, and zeta potential. The TPC for RPE, NPRP, and MNPRP was 240.3 ± 3.4, 191.7 ± 0.3, and 183.4 ± 2.1 mg EGA g(−1), while for TFC, the value was 37.8 ± 0.9, 35 ± 3.9, and 26.8 ± 1.9 mg EQ g(−1), respectively. Relevant antioxidant activity was also observed by FRAP, with 1400.2 (RPE), 1294.2 (NPRP), and 696.2 µmol Fe2+ g(−1) (MNPRP). The primary markers of RP were liquiritigenin, isoliquiritigenin, and formononetin. The particle sizes were 194.1 (NPs) and 361.2 nm (NPRP), with an encapsulation efficiency of 85.4%. Thermal analysis revealed high thermal stability for the PLA, nanoparticles, and membranes. The DSC revealed no interaction between the components. FTIR allowed for characterizing the RPE encapsulation in NPRP and CMC for the MNPRP. The membrane loaded with NPRP, fully characterized, has antioxidant capacity and may have application in the treatment of skin wounds.
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spelling pubmed-96092022022-10-28 Development of a Polymeric Membrane Impregnated with Poly-Lactic Acid (PLA) Nanoparticles Loaded with Red Propolis (RP) da Costa Silva, Valdemir do Nascimento, Ticiano G. Mergulhão, Naianny L. O. N. Freitas, Johnnatan D. Duarte, Ilza Fernanda B. de Bulhões, Laisa Carolina G. Dornelas, Camila B. de Araújo, João Xavier dos Santos, Jucenir Silva, Anielle C. A. Basílio, Irinaldo D. Goulart, Marilia O. F. Molecules Article The main objectives of this study were to develop and characterize hydrophilic polymeric membranes impregnated with poly-lactic acid (PLA) nanoparticles (NPs) combined with red propolis (RP). Ultrasonic-assisted extraction was used to obtain 30% (w/v) red propolis hydroalcoholic extract (RPE). The NPs (75,000 g mol(−1)) alone and incorporated with RP (NPRP) were obtained using the solvent emulsification and diffusion technique. Biopolymeric hydrogel membranes (MNPRP) were obtained using carboxymethylcellulose (CMC) and NPRP. Their characterization was performed using thermal analysis, Fourier transform infrared (FTIR), total phenols (TPC) and flavonoids contents (TFC), and antioxidant activity through the radical scavenging assay with 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and Ferric reducing antioxidant power (FRAP). The identification and quantification of significant RP markers were performed through UPLC-DAD. The NPs were evaluated for particle size, polydispersity index, and zeta potential. The TPC for RPE, NPRP, and MNPRP was 240.3 ± 3.4, 191.7 ± 0.3, and 183.4 ± 2.1 mg EGA g(−1), while for TFC, the value was 37.8 ± 0.9, 35 ± 3.9, and 26.8 ± 1.9 mg EQ g(−1), respectively. Relevant antioxidant activity was also observed by FRAP, with 1400.2 (RPE), 1294.2 (NPRP), and 696.2 µmol Fe2+ g(−1) (MNPRP). The primary markers of RP were liquiritigenin, isoliquiritigenin, and formononetin. The particle sizes were 194.1 (NPs) and 361.2 nm (NPRP), with an encapsulation efficiency of 85.4%. Thermal analysis revealed high thermal stability for the PLA, nanoparticles, and membranes. The DSC revealed no interaction between the components. FTIR allowed for characterizing the RPE encapsulation in NPRP and CMC for the MNPRP. The membrane loaded with NPRP, fully characterized, has antioxidant capacity and may have application in the treatment of skin wounds. MDPI 2022-10-17 /pmc/articles/PMC9609202/ /pubmed/36296550 http://dx.doi.org/10.3390/molecules27206959 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Costa Silva, Valdemir
do Nascimento, Ticiano G.
Mergulhão, Naianny L. O. N.
Freitas, Johnnatan D.
Duarte, Ilza Fernanda B.
de Bulhões, Laisa Carolina G.
Dornelas, Camila B.
de Araújo, João Xavier
dos Santos, Jucenir
Silva, Anielle C. A.
Basílio, Irinaldo D.
Goulart, Marilia O. F.
Development of a Polymeric Membrane Impregnated with Poly-Lactic Acid (PLA) Nanoparticles Loaded with Red Propolis (RP)
title Development of a Polymeric Membrane Impregnated with Poly-Lactic Acid (PLA) Nanoparticles Loaded with Red Propolis (RP)
title_full Development of a Polymeric Membrane Impregnated with Poly-Lactic Acid (PLA) Nanoparticles Loaded with Red Propolis (RP)
title_fullStr Development of a Polymeric Membrane Impregnated with Poly-Lactic Acid (PLA) Nanoparticles Loaded with Red Propolis (RP)
title_full_unstemmed Development of a Polymeric Membrane Impregnated with Poly-Lactic Acid (PLA) Nanoparticles Loaded with Red Propolis (RP)
title_short Development of a Polymeric Membrane Impregnated with Poly-Lactic Acid (PLA) Nanoparticles Loaded with Red Propolis (RP)
title_sort development of a polymeric membrane impregnated with poly-lactic acid (pla) nanoparticles loaded with red propolis (rp)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609202/
https://www.ncbi.nlm.nih.gov/pubmed/36296550
http://dx.doi.org/10.3390/molecules27206959
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