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Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient

Pyrogallol is an ingredient in hair dye. Its concentration in hair dye is managed at less than 2.0% in Korea. There have been no reports on the dermal absorption rate of pyrogallol. The two purposes of this study were to develop an analytical method and determine the dermal absorption rate of pyroga...

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Autores principales: Kim, Yu-Jin, Kim, Hyang-Yeon, Lee, Jung-Dae, Kim, Hong-Yoon, Im, Jueng-Eun, Kim, Kyu-Bong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609326/
https://www.ncbi.nlm.nih.gov/pubmed/36287850
http://dx.doi.org/10.3390/toxics10100570
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author Kim, Yu-Jin
Kim, Hyang-Yeon
Lee, Jung-Dae
Kim, Hong-Yoon
Im, Jueng-Eun
Kim, Kyu-Bong
author_facet Kim, Yu-Jin
Kim, Hyang-Yeon
Lee, Jung-Dae
Kim, Hong-Yoon
Im, Jueng-Eun
Kim, Kyu-Bong
author_sort Kim, Yu-Jin
collection PubMed
description Pyrogallol is an ingredient in hair dye. Its concentration in hair dye is managed at less than 2.0% in Korea. There have been no reports on the dermal absorption rate of pyrogallol. The two purposes of this study were to develop an analytical method and determine the dermal absorption rate of pyrogallol. An analytical method was developed and validated by high-performance liquid chromatography (HPLC) analysis of various matrices including swabs (SWAB), skin (SKIN, dermis + epidermis), stratum corneum (SC), and receptor fluid (RF). Linearity (r(2) = 0.9993–0.9998), accuracy (92.1–108.2%), and precision (0.5–9.5%) met the validation criteria in guidelines. A Franz diffusion cell was used to determine the dermal absorption of pyrogallol using the skin of mini pigs. Pyrogallol (2.0%) was applied to the skin (10 μL/cm(2)). For the actual hair dye conditions, the skin was wiped with a swab 30 min after application. Twenty-four hours later, it was wiped with a swab again and the SC was collected using tape stripping. All samples were extracted with water and analyzed. RF was recovered at 0, 1, 2, 4, 8, 12, and 24 h. The total dermal absorption rate of pyrogallol was determined to be 26.0 ± 3.9%.
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spelling pubmed-96093262022-10-28 Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient Kim, Yu-Jin Kim, Hyang-Yeon Lee, Jung-Dae Kim, Hong-Yoon Im, Jueng-Eun Kim, Kyu-Bong Toxics Article Pyrogallol is an ingredient in hair dye. Its concentration in hair dye is managed at less than 2.0% in Korea. There have been no reports on the dermal absorption rate of pyrogallol. The two purposes of this study were to develop an analytical method and determine the dermal absorption rate of pyrogallol. An analytical method was developed and validated by high-performance liquid chromatography (HPLC) analysis of various matrices including swabs (SWAB), skin (SKIN, dermis + epidermis), stratum corneum (SC), and receptor fluid (RF). Linearity (r(2) = 0.9993–0.9998), accuracy (92.1–108.2%), and precision (0.5–9.5%) met the validation criteria in guidelines. A Franz diffusion cell was used to determine the dermal absorption of pyrogallol using the skin of mini pigs. Pyrogallol (2.0%) was applied to the skin (10 μL/cm(2)). For the actual hair dye conditions, the skin was wiped with a swab 30 min after application. Twenty-four hours later, it was wiped with a swab again and the SC was collected using tape stripping. All samples were extracted with water and analyzed. RF was recovered at 0, 1, 2, 4, 8, 12, and 24 h. The total dermal absorption rate of pyrogallol was determined to be 26.0 ± 3.9%. MDPI 2022-09-29 /pmc/articles/PMC9609326/ /pubmed/36287850 http://dx.doi.org/10.3390/toxics10100570 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Yu-Jin
Kim, Hyang-Yeon
Lee, Jung-Dae
Kim, Hong-Yoon
Im, Jueng-Eun
Kim, Kyu-Bong
Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient
title Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient
title_full Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient
title_fullStr Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient
title_full_unstemmed Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient
title_short Analytical Method Development and Dermal Absorption of Pyrogallol, a Hair Dye Ingredient
title_sort analytical method development and dermal absorption of pyrogallol, a hair dye ingredient
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609326/
https://www.ncbi.nlm.nih.gov/pubmed/36287850
http://dx.doi.org/10.3390/toxics10100570
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