Simultaneous Detection of RIG-1, MDA5, and IFIT-1 Expression Is a Convenient Tool for Evaluation of the Interferon-Mediated Response

In this study, we developed a novel, multiplex qPCR assay for simultaneous detection of RIG-1, MDA5, and IFIT-1 at the mRNA level. The assay was validated in A549 cells transfected with in vitro transcribed RNAs. Both exogenous RNA-GFP and self-amplifying (saRNA-GFP) induced significant expression o...

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Detalles Bibliográficos
Autores principales: Lozhkov, Alexey A., Plotnikova, Marina A., Egorova, Marya A., Baranovskaya, Irina L., Elpaeva, Ekaterina A., Klotchenko, Sergey A., Vasin, Andrey V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609481/
https://www.ncbi.nlm.nih.gov/pubmed/36298646
http://dx.doi.org/10.3390/v14102090

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609481/
https://www.ncbi.nlm.nih.gov/pubmed/36298646
http://dx.doi.org/10.3390/v14102090

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