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Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon
Several factors are associated with the development of different clinical forms of tuberculosis (TB). The present study evaluated epidemiological variables and cytokine levels in samples from 89 patients with TB (75 with pulmonary TB and 14 with extrapulmonary TB) and 45 controls. Cytokines were mea...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609616/ https://www.ncbi.nlm.nih.gov/pubmed/36296351 http://dx.doi.org/10.3390/microorganisms10102075 |
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author | Queiroz, Maria Alice Freitas Lima, Sandra Souza Amoras, Ednelza da Silva Graça de Sousa, Francisca Dayse Martins Souza, Iury de Paula Nunes, Juliana Abreu Lima Brasil-Costa, Igor Cayres-Vallinoto, Izaura Maria Vieira Ishak, Ricardo Vallinoto, Antonio Carlos Rosário |
author_facet | Queiroz, Maria Alice Freitas Lima, Sandra Souza Amoras, Ednelza da Silva Graça de Sousa, Francisca Dayse Martins Souza, Iury de Paula Nunes, Juliana Abreu Lima Brasil-Costa, Igor Cayres-Vallinoto, Izaura Maria Vieira Ishak, Ricardo Vallinoto, Antonio Carlos Rosário |
author_sort | Queiroz, Maria Alice Freitas |
collection | PubMed |
description | Several factors are associated with the development of different clinical forms of tuberculosis (TB). The present study evaluated epidemiological variables and cytokine levels in samples from 89 patients with TB (75 with pulmonary TB and 14 with extrapulmonary TB) and 45 controls. Cytokines were measured by flow cytometry (Human Th1/Th2/Th17 Cytometric Bead Array kit). The TB group had a higher frequency of individuals who were 39 years of age or older, married, with primary education or illiterate and had a lower family income (p < 0.05). All individuals with extrapulmonary TB reported that they were not working, and the main reasons were related to disease symptoms or treatment. The levels of IFN-γ (OR = 4.06) and IL-4 (OR = 2.62) were more likely to be elevated in the TB group (p = 0.05), and IFN-γ levels were lower in patients with extrapulmonary TB compared to those with pulmonary TB (OR = 0.11; p = 0.0050). The ROC curve was applied to investigate the diagnostic accuracy of IFN-γ levels between the different clinical forms of tuberculosis, resulting in high AUC (0.8661; p < 0.0001), sensitivity (93.85%) and specificity median (65.90%), suggesting that IFN-γ levels are useful to differentiate pulmonary TB from extrapulmonary TB. The dysregulation of pro- and anti-inflammatory cytokine levels represent a risk for the development of TB and contribute to the pathogenesis of the disease, especially variation in IFN-γ levels, which may determine protection or risk for extrapulmonary TB. |
format | Online Article Text |
id | pubmed-9609616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96096162022-10-28 Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon Queiroz, Maria Alice Freitas Lima, Sandra Souza Amoras, Ednelza da Silva Graça de Sousa, Francisca Dayse Martins Souza, Iury de Paula Nunes, Juliana Abreu Lima Brasil-Costa, Igor Cayres-Vallinoto, Izaura Maria Vieira Ishak, Ricardo Vallinoto, Antonio Carlos Rosário Microorganisms Article Several factors are associated with the development of different clinical forms of tuberculosis (TB). The present study evaluated epidemiological variables and cytokine levels in samples from 89 patients with TB (75 with pulmonary TB and 14 with extrapulmonary TB) and 45 controls. Cytokines were measured by flow cytometry (Human Th1/Th2/Th17 Cytometric Bead Array kit). The TB group had a higher frequency of individuals who were 39 years of age or older, married, with primary education or illiterate and had a lower family income (p < 0.05). All individuals with extrapulmonary TB reported that they were not working, and the main reasons were related to disease symptoms or treatment. The levels of IFN-γ (OR = 4.06) and IL-4 (OR = 2.62) were more likely to be elevated in the TB group (p = 0.05), and IFN-γ levels were lower in patients with extrapulmonary TB compared to those with pulmonary TB (OR = 0.11; p = 0.0050). The ROC curve was applied to investigate the diagnostic accuracy of IFN-γ levels between the different clinical forms of tuberculosis, resulting in high AUC (0.8661; p < 0.0001), sensitivity (93.85%) and specificity median (65.90%), suggesting that IFN-γ levels are useful to differentiate pulmonary TB from extrapulmonary TB. The dysregulation of pro- and anti-inflammatory cytokine levels represent a risk for the development of TB and contribute to the pathogenesis of the disease, especially variation in IFN-γ levels, which may determine protection or risk for extrapulmonary TB. MDPI 2022-10-20 /pmc/articles/PMC9609616/ /pubmed/36296351 http://dx.doi.org/10.3390/microorganisms10102075 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Queiroz, Maria Alice Freitas Lima, Sandra Souza Amoras, Ednelza da Silva Graça de Sousa, Francisca Dayse Martins Souza, Iury de Paula Nunes, Juliana Abreu Lima Brasil-Costa, Igor Cayres-Vallinoto, Izaura Maria Vieira Ishak, Ricardo Vallinoto, Antonio Carlos Rosário Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon |
title | Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon |
title_full | Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon |
title_fullStr | Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon |
title_full_unstemmed | Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon |
title_short | Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon |
title_sort | epidemiological and cytokine profile of patients with pulmonary and extrapulmonary tuberculosis in a population of the brazilian amazon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609616/ https://www.ncbi.nlm.nih.gov/pubmed/36296351 http://dx.doi.org/10.3390/microorganisms10102075 |
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