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Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon

Several factors are associated with the development of different clinical forms of tuberculosis (TB). The present study evaluated epidemiological variables and cytokine levels in samples from 89 patients with TB (75 with pulmonary TB and 14 with extrapulmonary TB) and 45 controls. Cytokines were mea...

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Autores principales: Queiroz, Maria Alice Freitas, Lima, Sandra Souza, Amoras, Ednelza da Silva Graça, de Sousa, Francisca Dayse Martins, Souza, Iury de Paula, Nunes, Juliana Abreu Lima, Brasil-Costa, Igor, Cayres-Vallinoto, Izaura Maria Vieira, Ishak, Ricardo, Vallinoto, Antonio Carlos Rosário
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609616/
https://www.ncbi.nlm.nih.gov/pubmed/36296351
http://dx.doi.org/10.3390/microorganisms10102075
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author Queiroz, Maria Alice Freitas
Lima, Sandra Souza
Amoras, Ednelza da Silva Graça
de Sousa, Francisca Dayse Martins
Souza, Iury de Paula
Nunes, Juliana Abreu Lima
Brasil-Costa, Igor
Cayres-Vallinoto, Izaura Maria Vieira
Ishak, Ricardo
Vallinoto, Antonio Carlos Rosário
author_facet Queiroz, Maria Alice Freitas
Lima, Sandra Souza
Amoras, Ednelza da Silva Graça
de Sousa, Francisca Dayse Martins
Souza, Iury de Paula
Nunes, Juliana Abreu Lima
Brasil-Costa, Igor
Cayres-Vallinoto, Izaura Maria Vieira
Ishak, Ricardo
Vallinoto, Antonio Carlos Rosário
author_sort Queiroz, Maria Alice Freitas
collection PubMed
description Several factors are associated with the development of different clinical forms of tuberculosis (TB). The present study evaluated epidemiological variables and cytokine levels in samples from 89 patients with TB (75 with pulmonary TB and 14 with extrapulmonary TB) and 45 controls. Cytokines were measured by flow cytometry (Human Th1/Th2/Th17 Cytometric Bead Array kit). The TB group had a higher frequency of individuals who were 39 years of age or older, married, with primary education or illiterate and had a lower family income (p < 0.05). All individuals with extrapulmonary TB reported that they were not working, and the main reasons were related to disease symptoms or treatment. The levels of IFN-γ (OR = 4.06) and IL-4 (OR = 2.62) were more likely to be elevated in the TB group (p = 0.05), and IFN-γ levels were lower in patients with extrapulmonary TB compared to those with pulmonary TB (OR = 0.11; p = 0.0050). The ROC curve was applied to investigate the diagnostic accuracy of IFN-γ levels between the different clinical forms of tuberculosis, resulting in high AUC (0.8661; p < 0.0001), sensitivity (93.85%) and specificity median (65.90%), suggesting that IFN-γ levels are useful to differentiate pulmonary TB from extrapulmonary TB. The dysregulation of pro- and anti-inflammatory cytokine levels represent a risk for the development of TB and contribute to the pathogenesis of the disease, especially variation in IFN-γ levels, which may determine protection or risk for extrapulmonary TB.
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spelling pubmed-96096162022-10-28 Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon Queiroz, Maria Alice Freitas Lima, Sandra Souza Amoras, Ednelza da Silva Graça de Sousa, Francisca Dayse Martins Souza, Iury de Paula Nunes, Juliana Abreu Lima Brasil-Costa, Igor Cayres-Vallinoto, Izaura Maria Vieira Ishak, Ricardo Vallinoto, Antonio Carlos Rosário Microorganisms Article Several factors are associated with the development of different clinical forms of tuberculosis (TB). The present study evaluated epidemiological variables and cytokine levels in samples from 89 patients with TB (75 with pulmonary TB and 14 with extrapulmonary TB) and 45 controls. Cytokines were measured by flow cytometry (Human Th1/Th2/Th17 Cytometric Bead Array kit). The TB group had a higher frequency of individuals who were 39 years of age or older, married, with primary education or illiterate and had a lower family income (p < 0.05). All individuals with extrapulmonary TB reported that they were not working, and the main reasons were related to disease symptoms or treatment. The levels of IFN-γ (OR = 4.06) and IL-4 (OR = 2.62) were more likely to be elevated in the TB group (p = 0.05), and IFN-γ levels were lower in patients with extrapulmonary TB compared to those with pulmonary TB (OR = 0.11; p = 0.0050). The ROC curve was applied to investigate the diagnostic accuracy of IFN-γ levels between the different clinical forms of tuberculosis, resulting in high AUC (0.8661; p < 0.0001), sensitivity (93.85%) and specificity median (65.90%), suggesting that IFN-γ levels are useful to differentiate pulmonary TB from extrapulmonary TB. The dysregulation of pro- and anti-inflammatory cytokine levels represent a risk for the development of TB and contribute to the pathogenesis of the disease, especially variation in IFN-γ levels, which may determine protection or risk for extrapulmonary TB. MDPI 2022-10-20 /pmc/articles/PMC9609616/ /pubmed/36296351 http://dx.doi.org/10.3390/microorganisms10102075 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Queiroz, Maria Alice Freitas
Lima, Sandra Souza
Amoras, Ednelza da Silva Graça
de Sousa, Francisca Dayse Martins
Souza, Iury de Paula
Nunes, Juliana Abreu Lima
Brasil-Costa, Igor
Cayres-Vallinoto, Izaura Maria Vieira
Ishak, Ricardo
Vallinoto, Antonio Carlos Rosário
Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon
title Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon
title_full Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon
title_fullStr Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon
title_full_unstemmed Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon
title_short Epidemiological and Cytokine Profile of Patients with Pulmonary and Extrapulmonary Tuberculosis in a Population of the Brazilian Amazon
title_sort epidemiological and cytokine profile of patients with pulmonary and extrapulmonary tuberculosis in a population of the brazilian amazon
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609616/
https://www.ncbi.nlm.nih.gov/pubmed/36296351
http://dx.doi.org/10.3390/microorganisms10102075
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