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Characterization of Grapevine Fanleaf Virus Isolates in ‘Chardonnay’ Vines Exhibiting Severe and Mild Symptoms in Two Vineyards

Fanleaf degeneration is a complex viral disease of Vitis spp. that detrimentally impacts fruit yield and reduces the productive lifespan of most vineyards worldwide. In France, its main causal agent is grapevine fanleaf virus (GFLV). In the past, field experiments were conducted to explore cross-pro...

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Autores principales: Kubina, Julie, Hily, Jean-Michel, Mustin, Pierre, Komar, Véronique, Garcia, Shahinez, Martin, Isabelle Rachel, Poulicard, Nils, Velt, Amandine, Bonnet, Véronique, Mercier, Laurence, Lemaire, Olivier, Vigne, Emmanuelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609649/
https://www.ncbi.nlm.nih.gov/pubmed/36298857
http://dx.doi.org/10.3390/v14102303
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author Kubina, Julie
Hily, Jean-Michel
Mustin, Pierre
Komar, Véronique
Garcia, Shahinez
Martin, Isabelle Rachel
Poulicard, Nils
Velt, Amandine
Bonnet, Véronique
Mercier, Laurence
Lemaire, Olivier
Vigne, Emmanuelle
author_facet Kubina, Julie
Hily, Jean-Michel
Mustin, Pierre
Komar, Véronique
Garcia, Shahinez
Martin, Isabelle Rachel
Poulicard, Nils
Velt, Amandine
Bonnet, Véronique
Mercier, Laurence
Lemaire, Olivier
Vigne, Emmanuelle
author_sort Kubina, Julie
collection PubMed
description Fanleaf degeneration is a complex viral disease of Vitis spp. that detrimentally impacts fruit yield and reduces the productive lifespan of most vineyards worldwide. In France, its main causal agent is grapevine fanleaf virus (GFLV). In the past, field experiments were conducted to explore cross-protection as a management strategy of fanleaf degeneration, but results were unsatisfactory because the mild virus strain negatively impacted fruit yield. In order to select new mild GFLV isolates, we examined two old ‘Chardonnay’ parcels harbouring vines with distinct phenotypes. Symptoms and agronomic performances were monitored over the four-year study on 21 individual vines that were classified into three categories: asymptomatic GFLV-free vines, GFLV-infected vines severely diseased and GFLV-infected vines displaying mild symptoms. The complete coding genomic sequences of GFLV isolates in infected vines was determined by high-throughput sequencing. Most grapevines were infected with multiple genetically divergent variants. While no specific molecular features were apparent for GFLV isolates from vines displaying mild symptoms, a genetic differentiation of GFLV populations depending on the vineyard parcel was observed. The mild symptomatic grapevines identified during this study were established in a greenhouse to recover GFLV variants of potential interest for cross-protection studies.
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spelling pubmed-96096492022-10-28 Characterization of Grapevine Fanleaf Virus Isolates in ‘Chardonnay’ Vines Exhibiting Severe and Mild Symptoms in Two Vineyards Kubina, Julie Hily, Jean-Michel Mustin, Pierre Komar, Véronique Garcia, Shahinez Martin, Isabelle Rachel Poulicard, Nils Velt, Amandine Bonnet, Véronique Mercier, Laurence Lemaire, Olivier Vigne, Emmanuelle Viruses Article Fanleaf degeneration is a complex viral disease of Vitis spp. that detrimentally impacts fruit yield and reduces the productive lifespan of most vineyards worldwide. In France, its main causal agent is grapevine fanleaf virus (GFLV). In the past, field experiments were conducted to explore cross-protection as a management strategy of fanleaf degeneration, but results were unsatisfactory because the mild virus strain negatively impacted fruit yield. In order to select new mild GFLV isolates, we examined two old ‘Chardonnay’ parcels harbouring vines with distinct phenotypes. Symptoms and agronomic performances were monitored over the four-year study on 21 individual vines that were classified into three categories: asymptomatic GFLV-free vines, GFLV-infected vines severely diseased and GFLV-infected vines displaying mild symptoms. The complete coding genomic sequences of GFLV isolates in infected vines was determined by high-throughput sequencing. Most grapevines were infected with multiple genetically divergent variants. While no specific molecular features were apparent for GFLV isolates from vines displaying mild symptoms, a genetic differentiation of GFLV populations depending on the vineyard parcel was observed. The mild symptomatic grapevines identified during this study were established in a greenhouse to recover GFLV variants of potential interest for cross-protection studies. MDPI 2022-10-20 /pmc/articles/PMC9609649/ /pubmed/36298857 http://dx.doi.org/10.3390/v14102303 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kubina, Julie
Hily, Jean-Michel
Mustin, Pierre
Komar, Véronique
Garcia, Shahinez
Martin, Isabelle Rachel
Poulicard, Nils
Velt, Amandine
Bonnet, Véronique
Mercier, Laurence
Lemaire, Olivier
Vigne, Emmanuelle
Characterization of Grapevine Fanleaf Virus Isolates in ‘Chardonnay’ Vines Exhibiting Severe and Mild Symptoms in Two Vineyards
title Characterization of Grapevine Fanleaf Virus Isolates in ‘Chardonnay’ Vines Exhibiting Severe and Mild Symptoms in Two Vineyards
title_full Characterization of Grapevine Fanleaf Virus Isolates in ‘Chardonnay’ Vines Exhibiting Severe and Mild Symptoms in Two Vineyards
title_fullStr Characterization of Grapevine Fanleaf Virus Isolates in ‘Chardonnay’ Vines Exhibiting Severe and Mild Symptoms in Two Vineyards
title_full_unstemmed Characterization of Grapevine Fanleaf Virus Isolates in ‘Chardonnay’ Vines Exhibiting Severe and Mild Symptoms in Two Vineyards
title_short Characterization of Grapevine Fanleaf Virus Isolates in ‘Chardonnay’ Vines Exhibiting Severe and Mild Symptoms in Two Vineyards
title_sort characterization of grapevine fanleaf virus isolates in ‘chardonnay’ vines exhibiting severe and mild symptoms in two vineyards
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609649/
https://www.ncbi.nlm.nih.gov/pubmed/36298857
http://dx.doi.org/10.3390/v14102303
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