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Development of an HPV Genotype Detection Platform Based on Aggregation-Induced Emission (AIE) and Flow-Through Hybridization Technologies

Genetic mutations can cause life-threatening diseases such as cancers and sickle cell anemia. Gene detection is thus of importance for disease-risk prediction or early diagnosis and treatment. Apart from genetic defects, gene detection techniques can also be applied to gene-related diseases with hig...

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Autores principales: Ma, Chun-Ho (Charlie), Li, Liejun, Cai, Shuheng, Lin, Pei, Lam, Wing-Ki (Kristy), Lee, Tsz-Him (Ronald), Kwok, Tsz-Kin (Ryan), Xie, Longxu, Kun, Tit-Sang (Tom), Tang, Ben-Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609701/
https://www.ncbi.nlm.nih.gov/pubmed/36296629
http://dx.doi.org/10.3390/molecules27207036
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author Ma, Chun-Ho (Charlie)
Li, Liejun
Cai, Shuheng
Lin, Pei
Lam, Wing-Ki (Kristy)
Lee, Tsz-Him (Ronald)
Kwok, Tsz-Kin (Ryan)
Xie, Longxu
Kun, Tit-Sang (Tom)
Tang, Ben-Zhong
author_facet Ma, Chun-Ho (Charlie)
Li, Liejun
Cai, Shuheng
Lin, Pei
Lam, Wing-Ki (Kristy)
Lee, Tsz-Him (Ronald)
Kwok, Tsz-Kin (Ryan)
Xie, Longxu
Kun, Tit-Sang (Tom)
Tang, Ben-Zhong
author_sort Ma, Chun-Ho (Charlie)
collection PubMed
description Genetic mutations can cause life-threatening diseases such as cancers and sickle cell anemia. Gene detection is thus of importance for disease-risk prediction or early diagnosis and treatment. Apart from genetic defects, gene detection techniques can also be applied to gene-related diseases with high risk to human health such as human papillomavirus (HPV) infection. HPV infection has been strongly linked to cervical cancer. To achieve a high-throughput HPV gene detection platform, the flow-through hybridization system appears to be one of the commercialized diagnostic techniques for this purpose. The flow-through hybridization technique is based on a vacuum-guided flow of DNA fragments which is continuously directed toward the oligoprobes that are immobilized on the testing membrane. However, the conventional colorimetric method and signal read-out approach suffers a problem of low sensitivity. On the contrary, fluorescence approaches allow more sensitive detection and broad sensing ranges. In this work, a fluorescent dye HCAP, which possesses aggregation-induced emission (AIE) properties and is responsive to alkaline phosphatase, was developed and applied to the flow-through hybridization platform to achieve HPV genome diagnosis of clinical samples. Also, an automatic membrane reader was constructed based on the AIE-based diagnosis platform which can identify the diagnostic result of patient DNA with a total concordance rate of 100% in the clinical trial.
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spelling pubmed-96097012022-10-28 Development of an HPV Genotype Detection Platform Based on Aggregation-Induced Emission (AIE) and Flow-Through Hybridization Technologies Ma, Chun-Ho (Charlie) Li, Liejun Cai, Shuheng Lin, Pei Lam, Wing-Ki (Kristy) Lee, Tsz-Him (Ronald) Kwok, Tsz-Kin (Ryan) Xie, Longxu Kun, Tit-Sang (Tom) Tang, Ben-Zhong Molecules Article Genetic mutations can cause life-threatening diseases such as cancers and sickle cell anemia. Gene detection is thus of importance for disease-risk prediction or early diagnosis and treatment. Apart from genetic defects, gene detection techniques can also be applied to gene-related diseases with high risk to human health such as human papillomavirus (HPV) infection. HPV infection has been strongly linked to cervical cancer. To achieve a high-throughput HPV gene detection platform, the flow-through hybridization system appears to be one of the commercialized diagnostic techniques for this purpose. The flow-through hybridization technique is based on a vacuum-guided flow of DNA fragments which is continuously directed toward the oligoprobes that are immobilized on the testing membrane. However, the conventional colorimetric method and signal read-out approach suffers a problem of low sensitivity. On the contrary, fluorescence approaches allow more sensitive detection and broad sensing ranges. In this work, a fluorescent dye HCAP, which possesses aggregation-induced emission (AIE) properties and is responsive to alkaline phosphatase, was developed and applied to the flow-through hybridization platform to achieve HPV genome diagnosis of clinical samples. Also, an automatic membrane reader was constructed based on the AIE-based diagnosis platform which can identify the diagnostic result of patient DNA with a total concordance rate of 100% in the clinical trial. MDPI 2022-10-18 /pmc/articles/PMC9609701/ /pubmed/36296629 http://dx.doi.org/10.3390/molecules27207036 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Chun-Ho (Charlie)
Li, Liejun
Cai, Shuheng
Lin, Pei
Lam, Wing-Ki (Kristy)
Lee, Tsz-Him (Ronald)
Kwok, Tsz-Kin (Ryan)
Xie, Longxu
Kun, Tit-Sang (Tom)
Tang, Ben-Zhong
Development of an HPV Genotype Detection Platform Based on Aggregation-Induced Emission (AIE) and Flow-Through Hybridization Technologies
title Development of an HPV Genotype Detection Platform Based on Aggregation-Induced Emission (AIE) and Flow-Through Hybridization Technologies
title_full Development of an HPV Genotype Detection Platform Based on Aggregation-Induced Emission (AIE) and Flow-Through Hybridization Technologies
title_fullStr Development of an HPV Genotype Detection Platform Based on Aggregation-Induced Emission (AIE) and Flow-Through Hybridization Technologies
title_full_unstemmed Development of an HPV Genotype Detection Platform Based on Aggregation-Induced Emission (AIE) and Flow-Through Hybridization Technologies
title_short Development of an HPV Genotype Detection Platform Based on Aggregation-Induced Emission (AIE) and Flow-Through Hybridization Technologies
title_sort development of an hpv genotype detection platform based on aggregation-induced emission (aie) and flow-through hybridization technologies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609701/
https://www.ncbi.nlm.nih.gov/pubmed/36296629
http://dx.doi.org/10.3390/molecules27207036
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