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Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus

African swine fever (ASF), the highly lethal swine infectious disease caused by the African swine fever virus (ASFV), is a great threat to the swine industry. There is no effective vaccine or diagnostic method to prevent and control this disease currently. The p30 protein of ASFV is an important tar...

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Autores principales: Wang, Mengxiang, Song, Jinxing, Sun, Junru, Du, Yongkun, Qin, Xiaodong, Xia, Lu, Wu, Yanan, Zhang, Gaiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609742/
https://www.ncbi.nlm.nih.gov/pubmed/36298725
http://dx.doi.org/10.3390/v14102170
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author Wang, Mengxiang
Song, Jinxing
Sun, Junru
Du, Yongkun
Qin, Xiaodong
Xia, Lu
Wu, Yanan
Zhang, Gaiping
author_facet Wang, Mengxiang
Song, Jinxing
Sun, Junru
Du, Yongkun
Qin, Xiaodong
Xia, Lu
Wu, Yanan
Zhang, Gaiping
author_sort Wang, Mengxiang
collection PubMed
description African swine fever (ASF), the highly lethal swine infectious disease caused by the African swine fever virus (ASFV), is a great threat to the swine industry. There is no effective vaccine or diagnostic method to prevent and control this disease currently. The p30 protein of ASFV is an important target for serological diagnosis, expressed in the early stage of viral replication and has high immunogenicity and sequence conservatism. Here, the CP204L gene was cloned into the expression vector pET-30a (+), and the soluble p30 protein was successfully expressed in the E. coli prokaryotic expression system and then labeled with horseradish peroxidase (HRP) to be the enzyme-labeled antigen. Using the purified recombinant p30 protein, a double-antigen sandwich ELISA for ASFV antibody detection was developed. This method exhibits excellent specificity, sensitivity and reproducibility in clinical sample detection with lower cost and shorter production cycles. Taken together, this study provides technical support for antibody detection for ASFV.
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spelling pubmed-96097422022-10-28 Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus Wang, Mengxiang Song, Jinxing Sun, Junru Du, Yongkun Qin, Xiaodong Xia, Lu Wu, Yanan Zhang, Gaiping Viruses Article African swine fever (ASF), the highly lethal swine infectious disease caused by the African swine fever virus (ASFV), is a great threat to the swine industry. There is no effective vaccine or diagnostic method to prevent and control this disease currently. The p30 protein of ASFV is an important target for serological diagnosis, expressed in the early stage of viral replication and has high immunogenicity and sequence conservatism. Here, the CP204L gene was cloned into the expression vector pET-30a (+), and the soluble p30 protein was successfully expressed in the E. coli prokaryotic expression system and then labeled with horseradish peroxidase (HRP) to be the enzyme-labeled antigen. Using the purified recombinant p30 protein, a double-antigen sandwich ELISA for ASFV antibody detection was developed. This method exhibits excellent specificity, sensitivity and reproducibility in clinical sample detection with lower cost and shorter production cycles. Taken together, this study provides technical support for antibody detection for ASFV. MDPI 2022-09-30 /pmc/articles/PMC9609742/ /pubmed/36298725 http://dx.doi.org/10.3390/v14102170 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Mengxiang
Song, Jinxing
Sun, Junru
Du, Yongkun
Qin, Xiaodong
Xia, Lu
Wu, Yanan
Zhang, Gaiping
Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus
title Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus
title_full Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus
title_fullStr Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus
title_full_unstemmed Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus
title_short Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus
title_sort development of an effective double antigen sandwich elisa based on p30 protein to detect antibodies against african swine fever virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609742/
https://www.ncbi.nlm.nih.gov/pubmed/36298725
http://dx.doi.org/10.3390/v14102170
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