Cargando…

Chemical Composition, Antifungal and Anti-Biofilm Activities of Volatile Fractions of Convolvulus althaeoides L. Roots from Tunisia

The antifungal drugs currently available and mostly used for the treatment of candidiasis exhibit the phenomena of toxicity and increasing resistance. In this context, plant materials might represent promising sources of antifungal agents. The aim of this study is to evaluate for the first time the...

Descripción completa

Detalles Bibliográficos
Autores principales: Hrichi, Soukaina, Chaâbane-Banaoues, Raja, Alibrando, Filippo, Altemimi, Ammar B., Babba, Oussama, Majdoub, Yassine Oulad El, Nasri, Habib, Mondello, Luigi, Babba, Hamouda, Mighri, Zine, Cacciola, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609869/
https://www.ncbi.nlm.nih.gov/pubmed/36296427
http://dx.doi.org/10.3390/molecules27206834
_version_ 1784819128372035584
author Hrichi, Soukaina
Chaâbane-Banaoues, Raja
Alibrando, Filippo
Altemimi, Ammar B.
Babba, Oussama
Majdoub, Yassine Oulad El
Nasri, Habib
Mondello, Luigi
Babba, Hamouda
Mighri, Zine
Cacciola, Francesco
author_facet Hrichi, Soukaina
Chaâbane-Banaoues, Raja
Alibrando, Filippo
Altemimi, Ammar B.
Babba, Oussama
Majdoub, Yassine Oulad El
Nasri, Habib
Mondello, Luigi
Babba, Hamouda
Mighri, Zine
Cacciola, Francesco
author_sort Hrichi, Soukaina
collection PubMed
description The antifungal drugs currently available and mostly used for the treatment of candidiasis exhibit the phenomena of toxicity and increasing resistance. In this context, plant materials might represent promising sources of antifungal agents. The aim of this study is to evaluate for the first time the chemical content of the volatile fractions (VFs) along with the antifungal and anti-biofilm of Convolvulus althaeoides L. roots. The chemical composition was determined by gas chromatography coupled to a flame ionization detector and mass spectrometry. In total, 73 and 86 chemical compounds were detected in the n-hexane (VF1) and chloroform (VF2) fractions, respectively. Analysis revealed the presence of four main compounds: n-hexadecenoic acid (29.77%), 4-vinyl guaiacol (12.2%), bis(2-ethylhexyl)-adipate (9.69%) and eicosane (3.98%) in the VF extracted by hexane (VF1). n-hexadecenoic acid (34.04%), benzyl alcohol (7.86%) and linoleic acid (7.30%) were the main compounds found in the VF extracted with chloroform (VF2). The antifungal minimum inhibitory concentrations (MICs) of the obtained fractions against Candida albicans, Candida glabrata and Candida tropicalis were determined by the micro-dilution technique and values against Candida spp. ranged from 0.87 to 3.5 mg/mL. The biofilm inhibitory concentrations (IBF) and sustained inhibition (BSI) assays on C. albicans, C. glabrata and C. tropicalis were also investigated. The VFs inhibited biofilm formation up to 0.87 mg/mL for C. albicans, up to 1.75 mg/mL against C. glabrata and up to 0.87 mg/mL against C. tropicalis. The obtained results highlighted the synergistic mechanism of the detected molecules in the prevention of candidosic biofilm formation.
format Online
Article
Text
id pubmed-9609869
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96098692022-10-28 Chemical Composition, Antifungal and Anti-Biofilm Activities of Volatile Fractions of Convolvulus althaeoides L. Roots from Tunisia Hrichi, Soukaina Chaâbane-Banaoues, Raja Alibrando, Filippo Altemimi, Ammar B. Babba, Oussama Majdoub, Yassine Oulad El Nasri, Habib Mondello, Luigi Babba, Hamouda Mighri, Zine Cacciola, Francesco Molecules Article The antifungal drugs currently available and mostly used for the treatment of candidiasis exhibit the phenomena of toxicity and increasing resistance. In this context, plant materials might represent promising sources of antifungal agents. The aim of this study is to evaluate for the first time the chemical content of the volatile fractions (VFs) along with the antifungal and anti-biofilm of Convolvulus althaeoides L. roots. The chemical composition was determined by gas chromatography coupled to a flame ionization detector and mass spectrometry. In total, 73 and 86 chemical compounds were detected in the n-hexane (VF1) and chloroform (VF2) fractions, respectively. Analysis revealed the presence of four main compounds: n-hexadecenoic acid (29.77%), 4-vinyl guaiacol (12.2%), bis(2-ethylhexyl)-adipate (9.69%) and eicosane (3.98%) in the VF extracted by hexane (VF1). n-hexadecenoic acid (34.04%), benzyl alcohol (7.86%) and linoleic acid (7.30%) were the main compounds found in the VF extracted with chloroform (VF2). The antifungal minimum inhibitory concentrations (MICs) of the obtained fractions against Candida albicans, Candida glabrata and Candida tropicalis were determined by the micro-dilution technique and values against Candida spp. ranged from 0.87 to 3.5 mg/mL. The biofilm inhibitory concentrations (IBF) and sustained inhibition (BSI) assays on C. albicans, C. glabrata and C. tropicalis were also investigated. The VFs inhibited biofilm formation up to 0.87 mg/mL for C. albicans, up to 1.75 mg/mL against C. glabrata and up to 0.87 mg/mL against C. tropicalis. The obtained results highlighted the synergistic mechanism of the detected molecules in the prevention of candidosic biofilm formation. MDPI 2022-10-12 /pmc/articles/PMC9609869/ /pubmed/36296427 http://dx.doi.org/10.3390/molecules27206834 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hrichi, Soukaina
Chaâbane-Banaoues, Raja
Alibrando, Filippo
Altemimi, Ammar B.
Babba, Oussama
Majdoub, Yassine Oulad El
Nasri, Habib
Mondello, Luigi
Babba, Hamouda
Mighri, Zine
Cacciola, Francesco
Chemical Composition, Antifungal and Anti-Biofilm Activities of Volatile Fractions of Convolvulus althaeoides L. Roots from Tunisia
title Chemical Composition, Antifungal and Anti-Biofilm Activities of Volatile Fractions of Convolvulus althaeoides L. Roots from Tunisia
title_full Chemical Composition, Antifungal and Anti-Biofilm Activities of Volatile Fractions of Convolvulus althaeoides L. Roots from Tunisia
title_fullStr Chemical Composition, Antifungal and Anti-Biofilm Activities of Volatile Fractions of Convolvulus althaeoides L. Roots from Tunisia
title_full_unstemmed Chemical Composition, Antifungal and Anti-Biofilm Activities of Volatile Fractions of Convolvulus althaeoides L. Roots from Tunisia
title_short Chemical Composition, Antifungal and Anti-Biofilm Activities of Volatile Fractions of Convolvulus althaeoides L. Roots from Tunisia
title_sort chemical composition, antifungal and anti-biofilm activities of volatile fractions of convolvulus althaeoides l. roots from tunisia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9609869/
https://www.ncbi.nlm.nih.gov/pubmed/36296427
http://dx.doi.org/10.3390/molecules27206834
work_keys_str_mv AT hrichisoukaina chemicalcompositionantifungalandantibiofilmactivitiesofvolatilefractionsofconvolvulusalthaeoideslrootsfromtunisia
AT chaabanebanaouesraja chemicalcompositionantifungalandantibiofilmactivitiesofvolatilefractionsofconvolvulusalthaeoideslrootsfromtunisia
AT alibrandofilippo chemicalcompositionantifungalandantibiofilmactivitiesofvolatilefractionsofconvolvulusalthaeoideslrootsfromtunisia
AT altemimiammarb chemicalcompositionantifungalandantibiofilmactivitiesofvolatilefractionsofconvolvulusalthaeoideslrootsfromtunisia
AT babbaoussama chemicalcompositionantifungalandantibiofilmactivitiesofvolatilefractionsofconvolvulusalthaeoideslrootsfromtunisia
AT majdoubyassineouladel chemicalcompositionantifungalandantibiofilmactivitiesofvolatilefractionsofconvolvulusalthaeoideslrootsfromtunisia
AT nasrihabib chemicalcompositionantifungalandantibiofilmactivitiesofvolatilefractionsofconvolvulusalthaeoideslrootsfromtunisia
AT mondelloluigi chemicalcompositionantifungalandantibiofilmactivitiesofvolatilefractionsofconvolvulusalthaeoideslrootsfromtunisia
AT babbahamouda chemicalcompositionantifungalandantibiofilmactivitiesofvolatilefractionsofconvolvulusalthaeoideslrootsfromtunisia
AT mighrizine chemicalcompositionantifungalandantibiofilmactivitiesofvolatilefractionsofconvolvulusalthaeoideslrootsfromtunisia
AT cacciolafrancesco chemicalcompositionantifungalandantibiofilmactivitiesofvolatilefractionsofconvolvulusalthaeoideslrootsfromtunisia