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Salivary Exosomal MicroRNA-486-5p and MicroRNA-10b-5p in Oral and Oropharyngeal Squamous Cell Carcinoma
Background and Objectives: The research aimed at evaluating the capacity of salivary exosomal miR-10b-5p and miR-486-5p for oral and oropharyngeal cancer detection. Materials and Methods: The saliva samples were harvested from histopathological diagnosed oral and oropharyngeal squamous cell carcinom...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610161/ https://www.ncbi.nlm.nih.gov/pubmed/36295638 http://dx.doi.org/10.3390/medicina58101478 |
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author | Faur, Cosmin Ioan Roman, Rareș Călin Jurj, Ancuța Raduly, Lajos Almășan, Oana Rotaru, Horațiu Chirilă, Magdalena Moldovan, Mădălina Anca Hedeșiu, Mihaela Dinu, Cristian |
author_facet | Faur, Cosmin Ioan Roman, Rareș Călin Jurj, Ancuța Raduly, Lajos Almășan, Oana Rotaru, Horațiu Chirilă, Magdalena Moldovan, Mădălina Anca Hedeșiu, Mihaela Dinu, Cristian |
author_sort | Faur, Cosmin Ioan |
collection | PubMed |
description | Background and Objectives: The research aimed at evaluating the capacity of salivary exosomal miR-10b-5p and miR-486-5p for oral and oropharyngeal cancer detection. Materials and Methods: The saliva samples were harvested from histopathological diagnosed oral and oropharyngeal squamous cell carcinoma patients and healthy volunteer subjects. The exosomes were isolated by differential ultracentrifugation and quantified by Nano Track Analysis. The microRNAs were extracted and quantified from salivary exosomes by quantitative Real-Time Polymerase Chain Reaction. Results: This research comprised fifty participants. When compared to healthy controls, salivary exosomal miR-486-5p was elevated and miR-10b-5p was reduced in oral and oropharyngeal squamous cell carcinoma. Moreover, miR-486-5p had a high expression level in stage II of cancer in comparison to the other cancer stages. The cancer samples presented an increased exosome dimension compared to the control samples. Conclusions: Salivary exosomal miR-10b-5p and miR-486-5p have an altered expression in oral and oropharyngeal cancer. |
format | Online Article Text |
id | pubmed-9610161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96101612022-10-28 Salivary Exosomal MicroRNA-486-5p and MicroRNA-10b-5p in Oral and Oropharyngeal Squamous Cell Carcinoma Faur, Cosmin Ioan Roman, Rareș Călin Jurj, Ancuța Raduly, Lajos Almășan, Oana Rotaru, Horațiu Chirilă, Magdalena Moldovan, Mădălina Anca Hedeșiu, Mihaela Dinu, Cristian Medicina (Kaunas) Article Background and Objectives: The research aimed at evaluating the capacity of salivary exosomal miR-10b-5p and miR-486-5p for oral and oropharyngeal cancer detection. Materials and Methods: The saliva samples were harvested from histopathological diagnosed oral and oropharyngeal squamous cell carcinoma patients and healthy volunteer subjects. The exosomes were isolated by differential ultracentrifugation and quantified by Nano Track Analysis. The microRNAs were extracted and quantified from salivary exosomes by quantitative Real-Time Polymerase Chain Reaction. Results: This research comprised fifty participants. When compared to healthy controls, salivary exosomal miR-486-5p was elevated and miR-10b-5p was reduced in oral and oropharyngeal squamous cell carcinoma. Moreover, miR-486-5p had a high expression level in stage II of cancer in comparison to the other cancer stages. The cancer samples presented an increased exosome dimension compared to the control samples. Conclusions: Salivary exosomal miR-10b-5p and miR-486-5p have an altered expression in oral and oropharyngeal cancer. MDPI 2022-10-18 /pmc/articles/PMC9610161/ /pubmed/36295638 http://dx.doi.org/10.3390/medicina58101478 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Faur, Cosmin Ioan Roman, Rareș Călin Jurj, Ancuța Raduly, Lajos Almășan, Oana Rotaru, Horațiu Chirilă, Magdalena Moldovan, Mădălina Anca Hedeșiu, Mihaela Dinu, Cristian Salivary Exosomal MicroRNA-486-5p and MicroRNA-10b-5p in Oral and Oropharyngeal Squamous Cell Carcinoma |
title | Salivary Exosomal MicroRNA-486-5p and MicroRNA-10b-5p in Oral and Oropharyngeal Squamous Cell Carcinoma |
title_full | Salivary Exosomal MicroRNA-486-5p and MicroRNA-10b-5p in Oral and Oropharyngeal Squamous Cell Carcinoma |
title_fullStr | Salivary Exosomal MicroRNA-486-5p and MicroRNA-10b-5p in Oral and Oropharyngeal Squamous Cell Carcinoma |
title_full_unstemmed | Salivary Exosomal MicroRNA-486-5p and MicroRNA-10b-5p in Oral and Oropharyngeal Squamous Cell Carcinoma |
title_short | Salivary Exosomal MicroRNA-486-5p and MicroRNA-10b-5p in Oral and Oropharyngeal Squamous Cell Carcinoma |
title_sort | salivary exosomal microrna-486-5p and microrna-10b-5p in oral and oropharyngeal squamous cell carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610161/ https://www.ncbi.nlm.nih.gov/pubmed/36295638 http://dx.doi.org/10.3390/medicina58101478 |
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