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SARS-CoV-2 air and surface contamination in residential settings

SARS-CoV-2 transmission occurs mainly indoors, through virus-laden airborne particles. Although the presence and infectivity of SARS-CoV-2 in aerosol are now acknowledged, the underlying circumstances for its occurrence are still under investigation. The contamination of domiciliary environments dur...

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Autores principales: Correia, Gil, Rodrigues, Luís, Afonso, Mariana, Mota, Marta, Oliveira, Joana, Soares, Rui, Tomás, Ana Luísa, Reichel, Anna, Silva, Patrícia M., Costa, José J., da Silva, Manuel Gameiro, Santos, Nuno C., Gonçalves, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610309/
https://www.ncbi.nlm.nih.gov/pubmed/36302823
http://dx.doi.org/10.1038/s41598-022-22679-y
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author Correia, Gil
Rodrigues, Luís
Afonso, Mariana
Mota, Marta
Oliveira, Joana
Soares, Rui
Tomás, Ana Luísa
Reichel, Anna
Silva, Patrícia M.
Costa, José J.
da Silva, Manuel Gameiro
Santos, Nuno C.
Gonçalves, Teresa
author_facet Correia, Gil
Rodrigues, Luís
Afonso, Mariana
Mota, Marta
Oliveira, Joana
Soares, Rui
Tomás, Ana Luísa
Reichel, Anna
Silva, Patrícia M.
Costa, José J.
da Silva, Manuel Gameiro
Santos, Nuno C.
Gonçalves, Teresa
author_sort Correia, Gil
collection PubMed
description SARS-CoV-2 transmission occurs mainly indoors, through virus-laden airborne particles. Although the presence and infectivity of SARS-CoV-2 in aerosol are now acknowledged, the underlying circumstances for its occurrence are still under investigation. The contamination of domiciliary environments during the isolation of SARS-CoV-2-infected patients in their respective rooms in individual houses and in a nursing home was investigated by collecting surface and air samples in these environments. Surface contamination was detected in different contexts, both on high and low-touch surfaces. To determine the presence of virus particles in the air, two sampling methodologies were used: air and deposition sampling. Positive deposition samples were found in sampling locations above the patient’s height, and SARS-CoV-2 RNA was detected in impactation air samples within a size fraction below 2.5 μm. Surface samples rendered the highest positivity rate and persistence for a longer period. The presence of aerosolized SARS-CoV-2 RNA occurred mainly in deposition samples and closer to symptom onset. To evaluate the infectivity of selected positive samples, SARS-CoV-2 viability assays were performed, but our study was not able to validate the virus viability. The presented results confirm the presence of aerosolized SARS-CoV-2 RNA in indoor compartments occupied by COVID-19 patients with mild symptoms, in the absence of aerosol-generating clinical procedures.
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spelling pubmed-96103092022-10-28 SARS-CoV-2 air and surface contamination in residential settings Correia, Gil Rodrigues, Luís Afonso, Mariana Mota, Marta Oliveira, Joana Soares, Rui Tomás, Ana Luísa Reichel, Anna Silva, Patrícia M. Costa, José J. da Silva, Manuel Gameiro Santos, Nuno C. Gonçalves, Teresa Sci Rep Article SARS-CoV-2 transmission occurs mainly indoors, through virus-laden airborne particles. Although the presence and infectivity of SARS-CoV-2 in aerosol are now acknowledged, the underlying circumstances for its occurrence are still under investigation. The contamination of domiciliary environments during the isolation of SARS-CoV-2-infected patients in their respective rooms in individual houses and in a nursing home was investigated by collecting surface and air samples in these environments. Surface contamination was detected in different contexts, both on high and low-touch surfaces. To determine the presence of virus particles in the air, two sampling methodologies were used: air and deposition sampling. Positive deposition samples were found in sampling locations above the patient’s height, and SARS-CoV-2 RNA was detected in impactation air samples within a size fraction below 2.5 μm. Surface samples rendered the highest positivity rate and persistence for a longer period. The presence of aerosolized SARS-CoV-2 RNA occurred mainly in deposition samples and closer to symptom onset. To evaluate the infectivity of selected positive samples, SARS-CoV-2 viability assays were performed, but our study was not able to validate the virus viability. The presented results confirm the presence of aerosolized SARS-CoV-2 RNA in indoor compartments occupied by COVID-19 patients with mild symptoms, in the absence of aerosol-generating clinical procedures. Nature Publishing Group UK 2022-10-27 /pmc/articles/PMC9610309/ /pubmed/36302823 http://dx.doi.org/10.1038/s41598-022-22679-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Correia, Gil
Rodrigues, Luís
Afonso, Mariana
Mota, Marta
Oliveira, Joana
Soares, Rui
Tomás, Ana Luísa
Reichel, Anna
Silva, Patrícia M.
Costa, José J.
da Silva, Manuel Gameiro
Santos, Nuno C.
Gonçalves, Teresa
SARS-CoV-2 air and surface contamination in residential settings
title SARS-CoV-2 air and surface contamination in residential settings
title_full SARS-CoV-2 air and surface contamination in residential settings
title_fullStr SARS-CoV-2 air and surface contamination in residential settings
title_full_unstemmed SARS-CoV-2 air and surface contamination in residential settings
title_short SARS-CoV-2 air and surface contamination in residential settings
title_sort sars-cov-2 air and surface contamination in residential settings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610309/
https://www.ncbi.nlm.nih.gov/pubmed/36302823
http://dx.doi.org/10.1038/s41598-022-22679-y
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