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Evaluation of Five Buffers for Inactivation of Monkeypox Virus and Feasibility of Virus Detection Using the Panther Fusion(®) Open Access System

Rapid diagnosis is key to containing viral outbreaks. However, for the current monkeypox outbreak the major deterrent to rapid testing is the requirement for higher biocontainment of potentially infectious monkeypox virus specimens. The current CDC guidelines require the DNA extraction process befor...

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Autores principales: Fischer, Robert J., Gallogly, Shane, Schulz, Jonathan E., van Doremalen, Neeltje, Munster, Vincent, Das, Sanchita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610623/
https://www.ncbi.nlm.nih.gov/pubmed/36298782
http://dx.doi.org/10.3390/v14102227
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author Fischer, Robert J.
Gallogly, Shane
Schulz, Jonathan E.
van Doremalen, Neeltje
Munster, Vincent
Das, Sanchita
author_facet Fischer, Robert J.
Gallogly, Shane
Schulz, Jonathan E.
van Doremalen, Neeltje
Munster, Vincent
Das, Sanchita
author_sort Fischer, Robert J.
collection PubMed
description Rapid diagnosis is key to containing viral outbreaks. However, for the current monkeypox outbreak the major deterrent to rapid testing is the requirement for higher biocontainment of potentially infectious monkeypox virus specimens. The current CDC guidelines require the DNA extraction process before PCR amplification to be performed under biosafety level 3 unless vaccinated personnel are performing assays. This increases the turn-around time and makes certain laboratories insufficiently equipped to handle specimens from patients with suspected monkeypox infection. We investigated the ability of five commercially available lysis buffers and heat for inactivation of monkeypox virus. We also optimized the use of monkeypox virus in Hologic(®) Panther Specimen Lysis Buffer for detection of virus in the Panther Fusion(®) Open Access System using published generic and clade specific monkeypox virus primers and probes.
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spelling pubmed-96106232022-10-28 Evaluation of Five Buffers for Inactivation of Monkeypox Virus and Feasibility of Virus Detection Using the Panther Fusion(®) Open Access System Fischer, Robert J. Gallogly, Shane Schulz, Jonathan E. van Doremalen, Neeltje Munster, Vincent Das, Sanchita Viruses Article Rapid diagnosis is key to containing viral outbreaks. However, for the current monkeypox outbreak the major deterrent to rapid testing is the requirement for higher biocontainment of potentially infectious monkeypox virus specimens. The current CDC guidelines require the DNA extraction process before PCR amplification to be performed under biosafety level 3 unless vaccinated personnel are performing assays. This increases the turn-around time and makes certain laboratories insufficiently equipped to handle specimens from patients with suspected monkeypox infection. We investigated the ability of five commercially available lysis buffers and heat for inactivation of monkeypox virus. We also optimized the use of monkeypox virus in Hologic(®) Panther Specimen Lysis Buffer for detection of virus in the Panther Fusion(®) Open Access System using published generic and clade specific monkeypox virus primers and probes. MDPI 2022-10-10 /pmc/articles/PMC9610623/ /pubmed/36298782 http://dx.doi.org/10.3390/v14102227 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fischer, Robert J.
Gallogly, Shane
Schulz, Jonathan E.
van Doremalen, Neeltje
Munster, Vincent
Das, Sanchita
Evaluation of Five Buffers for Inactivation of Monkeypox Virus and Feasibility of Virus Detection Using the Panther Fusion(®) Open Access System
title Evaluation of Five Buffers for Inactivation of Monkeypox Virus and Feasibility of Virus Detection Using the Panther Fusion(®) Open Access System
title_full Evaluation of Five Buffers for Inactivation of Monkeypox Virus and Feasibility of Virus Detection Using the Panther Fusion(®) Open Access System
title_fullStr Evaluation of Five Buffers for Inactivation of Monkeypox Virus and Feasibility of Virus Detection Using the Panther Fusion(®) Open Access System
title_full_unstemmed Evaluation of Five Buffers for Inactivation of Monkeypox Virus and Feasibility of Virus Detection Using the Panther Fusion(®) Open Access System
title_short Evaluation of Five Buffers for Inactivation of Monkeypox Virus and Feasibility of Virus Detection Using the Panther Fusion(®) Open Access System
title_sort evaluation of five buffers for inactivation of monkeypox virus and feasibility of virus detection using the panther fusion(®) open access system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610623/
https://www.ncbi.nlm.nih.gov/pubmed/36298782
http://dx.doi.org/10.3390/v14102227
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