Rolling circle amplification (RCA) -based biosensor system for the fluorescent detection of miR-129-2-3p miRNA

Herein, a versatile fluorescent bioanalysis platform for sensitive and specific screening of target miRNA (miR-129-2-3p) was innovatively designed by applying target-induced rolling circle amplification (RCA) for efficient signal amplification. Specifically, miR-129-2-3p was used as a ligation templ...

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Detalles Bibliográficos
Autores principales: Ye, Yan, Lin, Yao, Chi, Zilin, Zhang, Jiasheng, Cai, Fan, Zhu, Youzhi, Tang, Dianping, Lin, Qingqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2022
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610657/
https://www.ncbi.nlm.nih.gov/pubmed/36312756
http://dx.doi.org/10.7717/peerj.14257
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author Ye, Yan
Lin, Yao
Chi, Zilin
Zhang, Jiasheng
Cai, Fan
Zhu, Youzhi
Tang, Dianping
Lin, Qingqiang
author_facet Ye, Yan
Lin, Yao
Chi, Zilin
Zhang, Jiasheng
Cai, Fan
Zhu, Youzhi
Tang, Dianping
Lin, Qingqiang
author_sort Ye, Yan
collection PubMed
description Herein, a versatile fluorescent bioanalysis platform for sensitive and specific screening of target miRNA (miR-129-2-3p) was innovatively designed by applying target-induced rolling circle amplification (RCA) for efficient signal amplification. Specifically, miR-129-2-3p was used as a ligation template to facilitate its ligation with padlock probes, followed by an RCA reaction in the presence of phi29 DNA polymerase. The dsDNA fragments and products were stained by SYBR Green I and then detected by fluorescence spectrophotometry. As a result, miR-129-2-3p concentrations as low as 50 nM could be detected. Furthermore, the expression of miR-129-2-3p in breast cancer patients was about twice that in healthy people. Therefore, the results indicated that the RCA-based biosensor system could be a valuable platform for miRNA detection in clinical diagnosis and biomedical study.
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spelling pubmed-96106572022-10-28 Rolling circle amplification (RCA) -based biosensor system for the fluorescent detection of miR-129-2-3p miRNA Ye, Yan Lin, Yao Chi, Zilin Zhang, Jiasheng Cai, Fan Zhu, Youzhi Tang, Dianping Lin, Qingqiang PeerJ Biochemistry Herein, a versatile fluorescent bioanalysis platform for sensitive and specific screening of target miRNA (miR-129-2-3p) was innovatively designed by applying target-induced rolling circle amplification (RCA) for efficient signal amplification. Specifically, miR-129-2-3p was used as a ligation template to facilitate its ligation with padlock probes, followed by an RCA reaction in the presence of phi29 DNA polymerase. The dsDNA fragments and products were stained by SYBR Green I and then detected by fluorescence spectrophotometry. As a result, miR-129-2-3p concentrations as low as 50 nM could be detected. Furthermore, the expression of miR-129-2-3p in breast cancer patients was about twice that in healthy people. Therefore, the results indicated that the RCA-based biosensor system could be a valuable platform for miRNA detection in clinical diagnosis and biomedical study. PeerJ Inc. 2022-10-24 /pmc/articles/PMC9610657/ /pubmed/36312756 http://dx.doi.org/10.7717/peerj.14257 Text en ©2022 Ye et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Ye, Yan
Lin, Yao
Chi, Zilin
Zhang, Jiasheng
Cai, Fan
Zhu, Youzhi
Tang, Dianping
Lin, Qingqiang
Rolling circle amplification (RCA) -based biosensor system for the fluorescent detection of miR-129-2-3p miRNA
title Rolling circle amplification (RCA) -based biosensor system for the fluorescent detection of miR-129-2-3p miRNA
title_full Rolling circle amplification (RCA) -based biosensor system for the fluorescent detection of miR-129-2-3p miRNA
title_fullStr Rolling circle amplification (RCA) -based biosensor system for the fluorescent detection of miR-129-2-3p miRNA
title_full_unstemmed Rolling circle amplification (RCA) -based biosensor system for the fluorescent detection of miR-129-2-3p miRNA
title_short Rolling circle amplification (RCA) -based biosensor system for the fluorescent detection of miR-129-2-3p miRNA
title_sort rolling circle amplification (rca) -based biosensor system for the fluorescent detection of mir-129-2-3p mirna
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610657/
https://www.ncbi.nlm.nih.gov/pubmed/36312756
http://dx.doi.org/10.7717/peerj.14257
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