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CRISPR-Cas Genome Editing Technique for Fish Disease Management: Current Study and Future Perspective

Scientists have discovered many ways to treat bacteria, viruses, and parasites in aquaculture; however, there is still an impossibility in finding a permanent solution for all types of diseases. In that case, the CRISPR-Cas genome-editing technique can be the potential solution to preventing disease...

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Autores principales: Ferdous, Md. Akib, Islam, Sk Injamamul, Habib, Nasim, Almehmadi, Mazen, Allahyani, Mamdouh, Alsaiari, Ahad Amer, Shafie, Alaa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610719/
https://www.ncbi.nlm.nih.gov/pubmed/36296288
http://dx.doi.org/10.3390/microorganisms10102012
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author Ferdous, Md. Akib
Islam, Sk Injamamul
Habib, Nasim
Almehmadi, Mazen
Allahyani, Mamdouh
Alsaiari, Ahad Amer
Shafie, Alaa
author_facet Ferdous, Md. Akib
Islam, Sk Injamamul
Habib, Nasim
Almehmadi, Mazen
Allahyani, Mamdouh
Alsaiari, Ahad Amer
Shafie, Alaa
author_sort Ferdous, Md. Akib
collection PubMed
description Scientists have discovered many ways to treat bacteria, viruses, and parasites in aquaculture; however, there is still an impossibility in finding a permanent solution for all types of diseases. In that case, the CRISPR-Cas genome-editing technique can be the potential solution to preventing diseases for aquaculture sustainability. CRISPR-Cas is cheaper, easier, and more precise than the other existing genome-editing technologies and can be used as a new disease treatment tool to solve the far-reaching challenges in aquaculture. This technique may now be employed in novel ways, such as modifying a single nucleotide base or tagging a location in the DNA with a fluorescent protein. This review paper provides an informative discussion on adopting CRISPR technology in aquaculture disease management. Starting with the basic knowledge of CRISPR technology and phages, this study highlights the development of RNA-guided immunity to combat the Chilodonella protozoan group and nervous necrosis virus (NNV) in marine finfish. Additionally, we highlight the immunological application of CRISPR-Cas against bacterial diseases in channel catfish and the white spot syndrome virus (WSSV) in shrimp. In addition, the review summarizes a synthesis of bioinformatics tools used for CRISPR-Cas sgRNA design, and acceptable solutions are discussed, considering the limitations.
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spelling pubmed-96107192022-10-28 CRISPR-Cas Genome Editing Technique for Fish Disease Management: Current Study and Future Perspective Ferdous, Md. Akib Islam, Sk Injamamul Habib, Nasim Almehmadi, Mazen Allahyani, Mamdouh Alsaiari, Ahad Amer Shafie, Alaa Microorganisms Review Scientists have discovered many ways to treat bacteria, viruses, and parasites in aquaculture; however, there is still an impossibility in finding a permanent solution for all types of diseases. In that case, the CRISPR-Cas genome-editing technique can be the potential solution to preventing diseases for aquaculture sustainability. CRISPR-Cas is cheaper, easier, and more precise than the other existing genome-editing technologies and can be used as a new disease treatment tool to solve the far-reaching challenges in aquaculture. This technique may now be employed in novel ways, such as modifying a single nucleotide base or tagging a location in the DNA with a fluorescent protein. This review paper provides an informative discussion on adopting CRISPR technology in aquaculture disease management. Starting with the basic knowledge of CRISPR technology and phages, this study highlights the development of RNA-guided immunity to combat the Chilodonella protozoan group and nervous necrosis virus (NNV) in marine finfish. Additionally, we highlight the immunological application of CRISPR-Cas against bacterial diseases in channel catfish and the white spot syndrome virus (WSSV) in shrimp. In addition, the review summarizes a synthesis of bioinformatics tools used for CRISPR-Cas sgRNA design, and acceptable solutions are discussed, considering the limitations. MDPI 2022-10-12 /pmc/articles/PMC9610719/ /pubmed/36296288 http://dx.doi.org/10.3390/microorganisms10102012 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Ferdous, Md. Akib
Islam, Sk Injamamul
Habib, Nasim
Almehmadi, Mazen
Allahyani, Mamdouh
Alsaiari, Ahad Amer
Shafie, Alaa
CRISPR-Cas Genome Editing Technique for Fish Disease Management: Current Study and Future Perspective
title CRISPR-Cas Genome Editing Technique for Fish Disease Management: Current Study and Future Perspective
title_full CRISPR-Cas Genome Editing Technique for Fish Disease Management: Current Study and Future Perspective
title_fullStr CRISPR-Cas Genome Editing Technique for Fish Disease Management: Current Study and Future Perspective
title_full_unstemmed CRISPR-Cas Genome Editing Technique for Fish Disease Management: Current Study and Future Perspective
title_short CRISPR-Cas Genome Editing Technique for Fish Disease Management: Current Study and Future Perspective
title_sort crispr-cas genome editing technique for fish disease management: current study and future perspective
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610719/
https://www.ncbi.nlm.nih.gov/pubmed/36296288
http://dx.doi.org/10.3390/microorganisms10102012
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