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Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas
Zika virus (ZIKV) is a mosquito-borne flavivirus that has recently emerged as a global health threat. The rise in ZIKV infections has driven an increased incidence of neonates born with microcephaly or other neurological malformations. Therefore, screening for ZIKV infection can considerably impact...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610724/ https://www.ncbi.nlm.nih.gov/pubmed/36287995 http://dx.doi.org/10.3390/tropicalmed7100254 |
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author | Sittikul, Pichamon Sriburin, Pimolpachr Rattanamahaphoom, Jittraporn Limkittikul, Kriengsak Sirivichayakul, Chukiat Chatchen, Supawat |
author_facet | Sittikul, Pichamon Sriburin, Pimolpachr Rattanamahaphoom, Jittraporn Limkittikul, Kriengsak Sirivichayakul, Chukiat Chatchen, Supawat |
author_sort | Sittikul, Pichamon |
collection | PubMed |
description | Zika virus (ZIKV) is a mosquito-borne flavivirus that has recently emerged as a global health threat. The rise in ZIKV infections has driven an increased incidence of neonates born with microcephaly or other neurological malformations. Therefore, screening for ZIKV infection can considerably impact pregnant women, especially during the first trimester. The majority of ZIKV infections are mild or asymptomatic, and clinical diagnosis is inaccurate. Moreover, given the high level of cross-reactivity among flaviviruses, serological approaches to distinguish ZIKV from dengue virus (DENV) infections are complicated. We used the combination of DENV and ZIKV nonstructural protein 1 (NS1) IgG enzyme-linked immunosorbent assay (ELISA) and ZIKV NS1 blockade-of-binding (BOB) ELISA to test the convalescent sera of non-flavivirus, primary DENV, secondary DENV, and ZIKV infections. Our findings indicate that primary testing using a ZIKV NS1 IgG ELISA, the test of choice for large-scale ZIKV serosurvey studies, provided relatively high sensitivity. Moreover, the confirmation of positive ELISA results using the ZIKV NS1 BOB ELISA increased average specificity to 94.59% across serum samples. The combined use of two simple ELISAs for ZIKV serosurveys and the monitoring of ZIKV infection during pregnancy can elucidate the epidemiology, pathogenesis, and complications of ZIKV in DENV-endemic areas. |
format | Online Article Text |
id | pubmed-9610724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96107242022-10-28 Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas Sittikul, Pichamon Sriburin, Pimolpachr Rattanamahaphoom, Jittraporn Limkittikul, Kriengsak Sirivichayakul, Chukiat Chatchen, Supawat Trop Med Infect Dis Article Zika virus (ZIKV) is a mosquito-borne flavivirus that has recently emerged as a global health threat. The rise in ZIKV infections has driven an increased incidence of neonates born with microcephaly or other neurological malformations. Therefore, screening for ZIKV infection can considerably impact pregnant women, especially during the first trimester. The majority of ZIKV infections are mild or asymptomatic, and clinical diagnosis is inaccurate. Moreover, given the high level of cross-reactivity among flaviviruses, serological approaches to distinguish ZIKV from dengue virus (DENV) infections are complicated. We used the combination of DENV and ZIKV nonstructural protein 1 (NS1) IgG enzyme-linked immunosorbent assay (ELISA) and ZIKV NS1 blockade-of-binding (BOB) ELISA to test the convalescent sera of non-flavivirus, primary DENV, secondary DENV, and ZIKV infections. Our findings indicate that primary testing using a ZIKV NS1 IgG ELISA, the test of choice for large-scale ZIKV serosurvey studies, provided relatively high sensitivity. Moreover, the confirmation of positive ELISA results using the ZIKV NS1 BOB ELISA increased average specificity to 94.59% across serum samples. The combined use of two simple ELISAs for ZIKV serosurveys and the monitoring of ZIKV infection during pregnancy can elucidate the epidemiology, pathogenesis, and complications of ZIKV in DENV-endemic areas. MDPI 2022-09-21 /pmc/articles/PMC9610724/ /pubmed/36287995 http://dx.doi.org/10.3390/tropicalmed7100254 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sittikul, Pichamon Sriburin, Pimolpachr Rattanamahaphoom, Jittraporn Limkittikul, Kriengsak Sirivichayakul, Chukiat Chatchen, Supawat Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas |
title | Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas |
title_full | Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas |
title_fullStr | Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas |
title_full_unstemmed | Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas |
title_short | Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas |
title_sort | combining immunoassays to identify zika virus infection in dengue-endemic areas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610724/ https://www.ncbi.nlm.nih.gov/pubmed/36287995 http://dx.doi.org/10.3390/tropicalmed7100254 |
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