Cargando…

Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas

Zika virus (ZIKV) is a mosquito-borne flavivirus that has recently emerged as a global health threat. The rise in ZIKV infections has driven an increased incidence of neonates born with microcephaly or other neurological malformations. Therefore, screening for ZIKV infection can considerably impact...

Descripción completa

Detalles Bibliográficos
Autores principales: Sittikul, Pichamon, Sriburin, Pimolpachr, Rattanamahaphoom, Jittraporn, Limkittikul, Kriengsak, Sirivichayakul, Chukiat, Chatchen, Supawat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610724/
https://www.ncbi.nlm.nih.gov/pubmed/36287995
http://dx.doi.org/10.3390/tropicalmed7100254
_version_ 1784819347632422912
author Sittikul, Pichamon
Sriburin, Pimolpachr
Rattanamahaphoom, Jittraporn
Limkittikul, Kriengsak
Sirivichayakul, Chukiat
Chatchen, Supawat
author_facet Sittikul, Pichamon
Sriburin, Pimolpachr
Rattanamahaphoom, Jittraporn
Limkittikul, Kriengsak
Sirivichayakul, Chukiat
Chatchen, Supawat
author_sort Sittikul, Pichamon
collection PubMed
description Zika virus (ZIKV) is a mosquito-borne flavivirus that has recently emerged as a global health threat. The rise in ZIKV infections has driven an increased incidence of neonates born with microcephaly or other neurological malformations. Therefore, screening for ZIKV infection can considerably impact pregnant women, especially during the first trimester. The majority of ZIKV infections are mild or asymptomatic, and clinical diagnosis is inaccurate. Moreover, given the high level of cross-reactivity among flaviviruses, serological approaches to distinguish ZIKV from dengue virus (DENV) infections are complicated. We used the combination of DENV and ZIKV nonstructural protein 1 (NS1) IgG enzyme-linked immunosorbent assay (ELISA) and ZIKV NS1 blockade-of-binding (BOB) ELISA to test the convalescent sera of non-flavivirus, primary DENV, secondary DENV, and ZIKV infections. Our findings indicate that primary testing using a ZIKV NS1 IgG ELISA, the test of choice for large-scale ZIKV serosurvey studies, provided relatively high sensitivity. Moreover, the confirmation of positive ELISA results using the ZIKV NS1 BOB ELISA increased average specificity to 94.59% across serum samples. The combined use of two simple ELISAs for ZIKV serosurveys and the monitoring of ZIKV infection during pregnancy can elucidate the epidemiology, pathogenesis, and complications of ZIKV in DENV-endemic areas.
format Online
Article
Text
id pubmed-9610724
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96107242022-10-28 Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas Sittikul, Pichamon Sriburin, Pimolpachr Rattanamahaphoom, Jittraporn Limkittikul, Kriengsak Sirivichayakul, Chukiat Chatchen, Supawat Trop Med Infect Dis Article Zika virus (ZIKV) is a mosquito-borne flavivirus that has recently emerged as a global health threat. The rise in ZIKV infections has driven an increased incidence of neonates born with microcephaly or other neurological malformations. Therefore, screening for ZIKV infection can considerably impact pregnant women, especially during the first trimester. The majority of ZIKV infections are mild or asymptomatic, and clinical diagnosis is inaccurate. Moreover, given the high level of cross-reactivity among flaviviruses, serological approaches to distinguish ZIKV from dengue virus (DENV) infections are complicated. We used the combination of DENV and ZIKV nonstructural protein 1 (NS1) IgG enzyme-linked immunosorbent assay (ELISA) and ZIKV NS1 blockade-of-binding (BOB) ELISA to test the convalescent sera of non-flavivirus, primary DENV, secondary DENV, and ZIKV infections. Our findings indicate that primary testing using a ZIKV NS1 IgG ELISA, the test of choice for large-scale ZIKV serosurvey studies, provided relatively high sensitivity. Moreover, the confirmation of positive ELISA results using the ZIKV NS1 BOB ELISA increased average specificity to 94.59% across serum samples. The combined use of two simple ELISAs for ZIKV serosurveys and the monitoring of ZIKV infection during pregnancy can elucidate the epidemiology, pathogenesis, and complications of ZIKV in DENV-endemic areas. MDPI 2022-09-21 /pmc/articles/PMC9610724/ /pubmed/36287995 http://dx.doi.org/10.3390/tropicalmed7100254 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sittikul, Pichamon
Sriburin, Pimolpachr
Rattanamahaphoom, Jittraporn
Limkittikul, Kriengsak
Sirivichayakul, Chukiat
Chatchen, Supawat
Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas
title Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas
title_full Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas
title_fullStr Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas
title_full_unstemmed Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas
title_short Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas
title_sort combining immunoassays to identify zika virus infection in dengue-endemic areas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610724/
https://www.ncbi.nlm.nih.gov/pubmed/36287995
http://dx.doi.org/10.3390/tropicalmed7100254
work_keys_str_mv AT sittikulpichamon combiningimmunoassaystoidentifyzikavirusinfectionindengueendemicareas
AT sriburinpimolpachr combiningimmunoassaystoidentifyzikavirusinfectionindengueendemicareas
AT rattanamahaphoomjittraporn combiningimmunoassaystoidentifyzikavirusinfectionindengueendemicareas
AT limkittikulkriengsak combiningimmunoassaystoidentifyzikavirusinfectionindengueendemicareas
AT sirivichayakulchukiat combiningimmunoassaystoidentifyzikavirusinfectionindengueendemicareas
AT chatchensupawat combiningimmunoassaystoidentifyzikavirusinfectionindengueendemicareas