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Gre Factors Are Required for Biofilm Formation in Salmonella enterica Serovar Typhimurium by Targeting Transcription of the csgD Gene
Rdar biofilm formation of Salmonella typhimurium and Escherichia coli is a common ancient multicellular behavior relevant in cell–cell and inter-organism interactions equally, as in interaction with biotic and abiotic surfaces. With the expression of the characteristic extracellular matrix component...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610925/ https://www.ncbi.nlm.nih.gov/pubmed/36296197 http://dx.doi.org/10.3390/microorganisms10101921 |
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author | Gaviria-Cantin, Tania Vargas, Andrés Felipe Mouali, Youssef El Jiménez, Carlos Jonay Cimdins-Ahne, Annika Madrid, Cristina Römling, Ute Balsalobre, Carlos |
author_facet | Gaviria-Cantin, Tania Vargas, Andrés Felipe Mouali, Youssef El Jiménez, Carlos Jonay Cimdins-Ahne, Annika Madrid, Cristina Römling, Ute Balsalobre, Carlos |
author_sort | Gaviria-Cantin, Tania |
collection | PubMed |
description | Rdar biofilm formation of Salmonella typhimurium and Escherichia coli is a common ancient multicellular behavior relevant in cell–cell and inter-organism interactions equally, as in interaction with biotic and abiotic surfaces. With the expression of the characteristic extracellular matrix components amyloid curli fimbriae and the exopolysaccharide cellulose, the central hub for the delicate regulation of rdar morphotype expression is the orphan transcriptional regulator CsgD. Gre factors are ubiquitously interacting with RNA polymerase to selectively overcome transcriptional pausing. In this work, we found that GreA/GreB are required for expression of the csgD operon and consequently the rdar morphotype. The ability of the Gre factors to suppress transcriptional pausing and the 147 bp 5′-UTR of csgD are required for the stimulatory effect of the Gre factors on csgD expression. These novel mechanism(s) of regulation for the csgD operon might be relevant under specific stress conditions. |
format | Online Article Text |
id | pubmed-9610925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96109252022-10-28 Gre Factors Are Required for Biofilm Formation in Salmonella enterica Serovar Typhimurium by Targeting Transcription of the csgD Gene Gaviria-Cantin, Tania Vargas, Andrés Felipe Mouali, Youssef El Jiménez, Carlos Jonay Cimdins-Ahne, Annika Madrid, Cristina Römling, Ute Balsalobre, Carlos Microorganisms Article Rdar biofilm formation of Salmonella typhimurium and Escherichia coli is a common ancient multicellular behavior relevant in cell–cell and inter-organism interactions equally, as in interaction with biotic and abiotic surfaces. With the expression of the characteristic extracellular matrix components amyloid curli fimbriae and the exopolysaccharide cellulose, the central hub for the delicate regulation of rdar morphotype expression is the orphan transcriptional regulator CsgD. Gre factors are ubiquitously interacting with RNA polymerase to selectively overcome transcriptional pausing. In this work, we found that GreA/GreB are required for expression of the csgD operon and consequently the rdar morphotype. The ability of the Gre factors to suppress transcriptional pausing and the 147 bp 5′-UTR of csgD are required for the stimulatory effect of the Gre factors on csgD expression. These novel mechanism(s) of regulation for the csgD operon might be relevant under specific stress conditions. MDPI 2022-09-27 /pmc/articles/PMC9610925/ /pubmed/36296197 http://dx.doi.org/10.3390/microorganisms10101921 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gaviria-Cantin, Tania Vargas, Andrés Felipe Mouali, Youssef El Jiménez, Carlos Jonay Cimdins-Ahne, Annika Madrid, Cristina Römling, Ute Balsalobre, Carlos Gre Factors Are Required for Biofilm Formation in Salmonella enterica Serovar Typhimurium by Targeting Transcription of the csgD Gene |
title | Gre Factors Are Required for Biofilm Formation in Salmonella enterica Serovar Typhimurium by Targeting Transcription of the csgD Gene |
title_full | Gre Factors Are Required for Biofilm Formation in Salmonella enterica Serovar Typhimurium by Targeting Transcription of the csgD Gene |
title_fullStr | Gre Factors Are Required for Biofilm Formation in Salmonella enterica Serovar Typhimurium by Targeting Transcription of the csgD Gene |
title_full_unstemmed | Gre Factors Are Required for Biofilm Formation in Salmonella enterica Serovar Typhimurium by Targeting Transcription of the csgD Gene |
title_short | Gre Factors Are Required for Biofilm Formation in Salmonella enterica Serovar Typhimurium by Targeting Transcription of the csgD Gene |
title_sort | gre factors are required for biofilm formation in salmonella enterica serovar typhimurium by targeting transcription of the csgd gene |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9610925/ https://www.ncbi.nlm.nih.gov/pubmed/36296197 http://dx.doi.org/10.3390/microorganisms10101921 |
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