Assessment of Semen Respiratory Activity of Domesticated Species before and after Cryopreservation: Boars, Bulls, Stallions, Reindeers and Roosters

SIMPLE SUMMARY: Artificial insemination is actively used in animal husbandry. It is important to know the quality of the sperm for artificial insemination. One of the indicators of sperm quality can be an assessment of energy metabolism, since energy is needed for sperm to move and fertilize the egg. We studied the respiration rate in spermatozoa of different animal species: bulls, stallions, boars, reindeer and roosters. To determine the production of energy (ATP), the substance 2.4-dinetrophenol (2.4-DNP) was used, which stopped the production of ATP. Semen was assessed before and after freezing. The evaluation showed the same response to the addition of 2.4-DNP to the semen of different species, as well as a sufficient relationship between the reaction of semen respiration to the addition of 2.4-DNP and the fertilizing ability of sperm. At the same time, no relationship was found between the respiratory rate and fertility. The 2.4-DNP test can be a suitable additional measure of sperm quality. ABSTRACT: To assess sperm quality, it is important to evaluate energy metabolism. The test substance 2.4-dinitrophenol (2.4-DNP) is an agent for destroying oxidative phosphorylation. 2.4-DNP shuts off the production of adenosine triphosphate (ATP) from oxidation and then, the respiration rate increases. If the respiratory chain is damaged, there is little or no response to adding 2.4-DNP. The aim of this study was to analyze the respiratory activity and oxidative phosphorylation in semen before and after freezing and compare the obtained data with the fertilizing ability of sperm. There was a reduction in sperm respiration rates in all species after thawing. The respiration of spermatozoa of boars, bulls, stallions, reindeers and chicken showed responses to 2.4-dinitrophenol. The only difference is in the strength of the response to the test substance. After freezing and thawing, respiratory stimulation by 2.4-DNP decreased. The results of our study show that respiration rate is not correlated with pregnancy rates and egg fertility. However, there was a high correlation between the stimulation of respiration by 2.4-dinitrophenol and pregnancy rates. The test for an increase in respiration rate after adding 2.4-dinitrophenol could be a suitable test of the fertilizing ability of sperm.