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Synthetic Thermo-Responsive Terpolymers as Tunable Scaffolds for Cell Culture Applications

The use of tailored synthetic hydrogels for in vitro tissue culture and biomanufacturing provides the advantage of mimicking the cell microenvironment without issues of batch-to-batch variability. To that end, this work focused on the design, characterization, and preliminary evaluation of thermo-re...

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Autores principales: Lizana-Vasquez, Gaby D., Arrieta-Viana, Luis F., Mendez-Vega, Janet, Acevedo, Aldo, Torres-Lugo, Madeline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611013/
https://www.ncbi.nlm.nih.gov/pubmed/36297960
http://dx.doi.org/10.3390/polym14204379
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author Lizana-Vasquez, Gaby D.
Arrieta-Viana, Luis F.
Mendez-Vega, Janet
Acevedo, Aldo
Torres-Lugo, Madeline
author_facet Lizana-Vasquez, Gaby D.
Arrieta-Viana, Luis F.
Mendez-Vega, Janet
Acevedo, Aldo
Torres-Lugo, Madeline
author_sort Lizana-Vasquez, Gaby D.
collection PubMed
description The use of tailored synthetic hydrogels for in vitro tissue culture and biomanufacturing provides the advantage of mimicking the cell microenvironment without issues of batch-to-batch variability. To that end, this work focused on the design, characterization, and preliminary evaluation of thermo-responsive, transparent synthetic terpolymers based on N-isopropylacrylamide, vinylphenylboronic acid, and polyethylene glycol for cell manufacturing and in vitro culture applications. Polymer physical properties were characterized by FT-IR, (1)H-NMR, DLS, rheology, and thermal-gravimetric analysis. Tested combinations provided polymers with a lower critical solution temperature (LCST) between 30 and 45 °C. Terpolymer elastic/shear modulus varied between 0.3 and 19.1 kPa at 37 °C. Cellular characterization indicated low cell cytotoxicity on NIH-3T3. Experiments with the ovarian cancer model SKOV-3 and Jurkat T cells showed the terpolymers’ capacity for cell encapsulation without interfering with staining or imaging protocols. In addition, cell growth and high levels of pluripotency demonstrated the capability of terpolymer to culture iPSCs. Characterization results confirmed a promising use of terpolymers as a tunable scaffold for cell culture applications.
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spelling pubmed-96110132022-10-28 Synthetic Thermo-Responsive Terpolymers as Tunable Scaffolds for Cell Culture Applications Lizana-Vasquez, Gaby D. Arrieta-Viana, Luis F. Mendez-Vega, Janet Acevedo, Aldo Torres-Lugo, Madeline Polymers (Basel) Article The use of tailored synthetic hydrogels for in vitro tissue culture and biomanufacturing provides the advantage of mimicking the cell microenvironment without issues of batch-to-batch variability. To that end, this work focused on the design, characterization, and preliminary evaluation of thermo-responsive, transparent synthetic terpolymers based on N-isopropylacrylamide, vinylphenylboronic acid, and polyethylene glycol for cell manufacturing and in vitro culture applications. Polymer physical properties were characterized by FT-IR, (1)H-NMR, DLS, rheology, and thermal-gravimetric analysis. Tested combinations provided polymers with a lower critical solution temperature (LCST) between 30 and 45 °C. Terpolymer elastic/shear modulus varied between 0.3 and 19.1 kPa at 37 °C. Cellular characterization indicated low cell cytotoxicity on NIH-3T3. Experiments with the ovarian cancer model SKOV-3 and Jurkat T cells showed the terpolymers’ capacity for cell encapsulation without interfering with staining or imaging protocols. In addition, cell growth and high levels of pluripotency demonstrated the capability of terpolymer to culture iPSCs. Characterization results confirmed a promising use of terpolymers as a tunable scaffold for cell culture applications. MDPI 2022-10-17 /pmc/articles/PMC9611013/ /pubmed/36297960 http://dx.doi.org/10.3390/polym14204379 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lizana-Vasquez, Gaby D.
Arrieta-Viana, Luis F.
Mendez-Vega, Janet
Acevedo, Aldo
Torres-Lugo, Madeline
Synthetic Thermo-Responsive Terpolymers as Tunable Scaffolds for Cell Culture Applications
title Synthetic Thermo-Responsive Terpolymers as Tunable Scaffolds for Cell Culture Applications
title_full Synthetic Thermo-Responsive Terpolymers as Tunable Scaffolds for Cell Culture Applications
title_fullStr Synthetic Thermo-Responsive Terpolymers as Tunable Scaffolds for Cell Culture Applications
title_full_unstemmed Synthetic Thermo-Responsive Terpolymers as Tunable Scaffolds for Cell Culture Applications
title_short Synthetic Thermo-Responsive Terpolymers as Tunable Scaffolds for Cell Culture Applications
title_sort synthetic thermo-responsive terpolymers as tunable scaffolds for cell culture applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611013/
https://www.ncbi.nlm.nih.gov/pubmed/36297960
http://dx.doi.org/10.3390/polym14204379
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