Cargando…
Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon
The emergence of clinically relevant mutations in the hepatitis B virus (HBV) genome has been a matter of great debate because of the possibility of escape from the host’s immune system, the potential to cause more severe progression of liver diseases and the emergence of treatment-resistant variant...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611064/ https://www.ncbi.nlm.nih.gov/pubmed/36298655 http://dx.doi.org/10.3390/v14102100 |
_version_ | 1784819434099048448 |
---|---|
author | Roca, Tárcio Peixoto Villar, Livia Melo Nogueira Lima, Felipe Souza Vasconcelos, Mariana Pinheiro Alves Borzacov, Lourdes Maria Pinheiro Silva, Eugênia de Castro e do Lago, Bárbara Vieira da Silva, Mayara Torquato Lima Botelho Souza, Luan Felipo Salcedo, Juan Miguel Villalobos dos Santos, Alcione de Oliveira Vieira, Deusilene Souza |
author_facet | Roca, Tárcio Peixoto Villar, Livia Melo Nogueira Lima, Felipe Souza Vasconcelos, Mariana Pinheiro Alves Borzacov, Lourdes Maria Pinheiro Silva, Eugênia de Castro e do Lago, Bárbara Vieira da Silva, Mayara Torquato Lima Botelho Souza, Luan Felipo Salcedo, Juan Miguel Villalobos dos Santos, Alcione de Oliveira Vieira, Deusilene Souza |
author_sort | Roca, Tárcio Peixoto |
collection | PubMed |
description | The emergence of clinically relevant mutations in the hepatitis B virus (HBV) genome has been a matter of great debate because of the possibility of escape from the host’s immune system, the potential to cause more severe progression of liver diseases and the emergence of treatment-resistant variants. Here we characterized the circulating variants of HBV in Rondônia State, in the north of Brazil. Serum samples of 62 chronic HBV carriers were subjected to PCR assays and clinical data were collected. Mutations and genotypes were characterized through direct sequencing. The findings show the presence of subgenotypes A1 (54.83%, 34/62), D3 (16.13%, 10/62), F2 (16.13%, 10/62), A2 (4.84%, 3/62), D2 (3.23%, 2/62), D1 (1.61%, 1/62), D4 (1.61%, 1/62) and F4 (1.61%, 1/62). Deletions in the pre-S2 region were found in 13.79% (8/58) of the samples, mutations in the S gene in 59.68% (37/62) and RT mutations in 48.39% (30/62). We found a variable genotypic distribution in different locations and important mutations related to immune escape and drug resistance in Western Amazonia, which contributed to genetic surveillance and provided important information to help control the disease. |
format | Online Article Text |
id | pubmed-9611064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96110642022-10-28 Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon Roca, Tárcio Peixoto Villar, Livia Melo Nogueira Lima, Felipe Souza Vasconcelos, Mariana Pinheiro Alves Borzacov, Lourdes Maria Pinheiro Silva, Eugênia de Castro e do Lago, Bárbara Vieira da Silva, Mayara Torquato Lima Botelho Souza, Luan Felipo Salcedo, Juan Miguel Villalobos dos Santos, Alcione de Oliveira Vieira, Deusilene Souza Viruses Article The emergence of clinically relevant mutations in the hepatitis B virus (HBV) genome has been a matter of great debate because of the possibility of escape from the host’s immune system, the potential to cause more severe progression of liver diseases and the emergence of treatment-resistant variants. Here we characterized the circulating variants of HBV in Rondônia State, in the north of Brazil. Serum samples of 62 chronic HBV carriers were subjected to PCR assays and clinical data were collected. Mutations and genotypes were characterized through direct sequencing. The findings show the presence of subgenotypes A1 (54.83%, 34/62), D3 (16.13%, 10/62), F2 (16.13%, 10/62), A2 (4.84%, 3/62), D2 (3.23%, 2/62), D1 (1.61%, 1/62), D4 (1.61%, 1/62) and F4 (1.61%, 1/62). Deletions in the pre-S2 region were found in 13.79% (8/58) of the samples, mutations in the S gene in 59.68% (37/62) and RT mutations in 48.39% (30/62). We found a variable genotypic distribution in different locations and important mutations related to immune escape and drug resistance in Western Amazonia, which contributed to genetic surveillance and provided important information to help control the disease. MDPI 2022-09-22 /pmc/articles/PMC9611064/ /pubmed/36298655 http://dx.doi.org/10.3390/v14102100 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Roca, Tárcio Peixoto Villar, Livia Melo Nogueira Lima, Felipe Souza Vasconcelos, Mariana Pinheiro Alves Borzacov, Lourdes Maria Pinheiro Silva, Eugênia de Castro e do Lago, Bárbara Vieira da Silva, Mayara Torquato Lima Botelho Souza, Luan Felipo Salcedo, Juan Miguel Villalobos dos Santos, Alcione de Oliveira Vieira, Deusilene Souza Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon |
title | Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon |
title_full | Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon |
title_fullStr | Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon |
title_full_unstemmed | Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon |
title_short | Genomic Variability of Hepatitis B Virus Circulating in Brazilian Western Amazon |
title_sort | genomic variability of hepatitis b virus circulating in brazilian western amazon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611064/ https://www.ncbi.nlm.nih.gov/pubmed/36298655 http://dx.doi.org/10.3390/v14102100 |
work_keys_str_mv | AT rocatarciopeixoto genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon AT villarliviamelo genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon AT nogueiralimafelipesouza genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon AT vasconcelosmarianapinheiroalves genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon AT borzacovlourdesmariapinheiro genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon AT silvaeugeniadecastroe genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon AT dolagobarbaravieira genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon AT dasilvamayaratorquatolima genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon AT botelhosouzaluanfelipo genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon AT salcedojuanmiguelvillalobos genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon AT dossantosalcionedeoliveira genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon AT vieiradeusilenesouza genomicvariabilityofhepatitisbviruscirculatinginbrazilianwesternamazon |