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Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming Candida auris with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite

Candida auris (C. auris), an emerging multidrug-resistant microorganism, with limited therapeutical options, is one of the leading causes of nosocomial infections. The current study includes 19 C. auris strains collected from King Fahd Hospital of the University and King Fahad Specialist Hospital in...

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Autores principales: Aldossary, Hanan A., Rehman, Suriya, Jermy, B. Rabindran, AlJindan, Reem, Aldayel, Afra, AbdulAzeez, Sayed, Akhtar, Sultan, Khan, Firdos Alam, Borgio, J. Francis, Al-Suhaimi, Ebtesam Abdullah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611151/
https://www.ncbi.nlm.nih.gov/pubmed/36297684
http://dx.doi.org/10.3390/pharmaceutics14102251
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author Aldossary, Hanan A.
Rehman, Suriya
Jermy, B. Rabindran
AlJindan, Reem
Aldayel, Afra
AbdulAzeez, Sayed
Akhtar, Sultan
Khan, Firdos Alam
Borgio, J. Francis
Al-Suhaimi, Ebtesam Abdullah
author_facet Aldossary, Hanan A.
Rehman, Suriya
Jermy, B. Rabindran
AlJindan, Reem
Aldayel, Afra
AbdulAzeez, Sayed
Akhtar, Sultan
Khan, Firdos Alam
Borgio, J. Francis
Al-Suhaimi, Ebtesam Abdullah
author_sort Aldossary, Hanan A.
collection PubMed
description Candida auris (C. auris), an emerging multidrug-resistant microorganism, with limited therapeutical options, is one of the leading causes of nosocomial infections. The current study includes 19 C. auris strains collected from King Fahd Hospital of the University and King Fahad Specialist Hospital in Dammam, identified by 18S rRNA gene and ITS region sequencing. Drug-resistance-associated mutations in ERG11, TAC1B and FUR1 genes were screened to gain insight into the pattern of drug resistance. Molecular identification was successfully achieved using 18S rRNA gene and ITS region and 5 drug-resistance-associated missense variants identified in the ERG11 (F132Y and K143R) and TAC1B (H608Y, P611S and A640V) genes of C. auris strains, grouped into 3 clades. The prophylactic and therapeutic application of hydrothermally synthesized Ag-silicalite-1 (Si/Ag ratio 25) nanomaterial was tested against the 3 clades of clinical C. auris strains. 4wt%Ag/TiZSM-5 prepared using conventional impregnation technique was used for comparative study, and nano formulations were characterized using different techniques. The antibiofilm activity of nanomaterials was tested by cell kill assay, scanning electron microscopy (SEM) and light microscopy. Across all the clades of C. auris strains, 4 wt%Ag/TiZSM-5 and Ag-silicalite-1 demonstrated a significant (p = 1.1102 × 10(−16)) inhibitory effect on the biofilm’s survival rate: the lowest inhibition value was (10%) with Ag-silicalite-1 at 24 and 48 h incubation. A profound change in morphogenesis in addition to the reduction in the number of C. auris cells was shown by SEM and light microscopy. The presence of a high surface area and the uniform dispersion of nanosized Ag species displays enhanced anti-Candida activity, and therefore it has great potential against the emerging multidrug-resistant C. auris.
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spelling pubmed-96111512022-10-28 Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming Candida auris with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite Aldossary, Hanan A. Rehman, Suriya Jermy, B. Rabindran AlJindan, Reem Aldayel, Afra AbdulAzeez, Sayed Akhtar, Sultan Khan, Firdos Alam Borgio, J. Francis Al-Suhaimi, Ebtesam Abdullah Pharmaceutics Article Candida auris (C. auris), an emerging multidrug-resistant microorganism, with limited therapeutical options, is one of the leading causes of nosocomial infections. The current study includes 19 C. auris strains collected from King Fahd Hospital of the University and King Fahad Specialist Hospital in Dammam, identified by 18S rRNA gene and ITS region sequencing. Drug-resistance-associated mutations in ERG11, TAC1B and FUR1 genes were screened to gain insight into the pattern of drug resistance. Molecular identification was successfully achieved using 18S rRNA gene and ITS region and 5 drug-resistance-associated missense variants identified in the ERG11 (F132Y and K143R) and TAC1B (H608Y, P611S and A640V) genes of C. auris strains, grouped into 3 clades. The prophylactic and therapeutic application of hydrothermally synthesized Ag-silicalite-1 (Si/Ag ratio 25) nanomaterial was tested against the 3 clades of clinical C. auris strains. 4wt%Ag/TiZSM-5 prepared using conventional impregnation technique was used for comparative study, and nano formulations were characterized using different techniques. The antibiofilm activity of nanomaterials was tested by cell kill assay, scanning electron microscopy (SEM) and light microscopy. Across all the clades of C. auris strains, 4 wt%Ag/TiZSM-5 and Ag-silicalite-1 demonstrated a significant (p = 1.1102 × 10(−16)) inhibitory effect on the biofilm’s survival rate: the lowest inhibition value was (10%) with Ag-silicalite-1 at 24 and 48 h incubation. A profound change in morphogenesis in addition to the reduction in the number of C. auris cells was shown by SEM and light microscopy. The presence of a high surface area and the uniform dispersion of nanosized Ag species displays enhanced anti-Candida activity, and therefore it has great potential against the emerging multidrug-resistant C. auris. MDPI 2022-10-21 /pmc/articles/PMC9611151/ /pubmed/36297684 http://dx.doi.org/10.3390/pharmaceutics14102251 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aldossary, Hanan A.
Rehman, Suriya
Jermy, B. Rabindran
AlJindan, Reem
Aldayel, Afra
AbdulAzeez, Sayed
Akhtar, Sultan
Khan, Firdos Alam
Borgio, J. Francis
Al-Suhaimi, Ebtesam Abdullah
Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming Candida auris with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite
title Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming Candida auris with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite
title_full Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming Candida auris with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite
title_fullStr Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming Candida auris with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite
title_full_unstemmed Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming Candida auris with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite
title_short Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming Candida auris with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite
title_sort therapeutic intervention for various hospital setting strains of biofilm forming candida auris with multiple drug resistance mutations using nanomaterial ag-silicalite-1 zeolite
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611151/
https://www.ncbi.nlm.nih.gov/pubmed/36297684
http://dx.doi.org/10.3390/pharmaceutics14102251
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