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Consumption of Galactose by Trypanosoma cruzi Epimastigotes Generates Resistance against Oxidative Stress

In this study, we demonstrate that Trypanosoma cruzi epimastigotes previously grown in LIT medium supplemented with 20 mM galactose and exposed to sub-lethal concentrations of hydrogen peroxide (100 μM) showed two-fold and five-fold viability when compared to epimastigotes grown in LIT medium supple...

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Autores principales: Lobo-Rojas, Ángel, Quintero-Troconis, Ender, Rondón-Mercado, Rocío, Pérez-Aguilar., Mary Carmen, Concepción, Juan Luis, Cáceres, Ana Judith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611177/
https://www.ncbi.nlm.nih.gov/pubmed/36297231
http://dx.doi.org/10.3390/pathogens11101174
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author Lobo-Rojas, Ángel
Quintero-Troconis, Ender
Rondón-Mercado, Rocío
Pérez-Aguilar., Mary Carmen
Concepción, Juan Luis
Cáceres, Ana Judith
author_facet Lobo-Rojas, Ángel
Quintero-Troconis, Ender
Rondón-Mercado, Rocío
Pérez-Aguilar., Mary Carmen
Concepción, Juan Luis
Cáceres, Ana Judith
author_sort Lobo-Rojas, Ángel
collection PubMed
description In this study, we demonstrate that Trypanosoma cruzi epimastigotes previously grown in LIT medium supplemented with 20 mM galactose and exposed to sub-lethal concentrations of hydrogen peroxide (100 μM) showed two-fold and five-fold viability when compared to epimastigotes grown in LIT medium supplemented with two different glucose concentrations (20 mM and 1.5 mM), respectively. Similar results were obtained when exposing epimastigotes from all treatments to methylene blue 30 μM. Additionally, through differential centrifugation and the selective permeabilization of cellular membranes with digitonin, we found that phosphoglucomutase activity (a key enzyme in galactose metabolism) occurs predominantly within the cytosolic compartment. Furthermore, after partially permeabilizing epimastigotes with digitonin (0.025 mg × mg(−1) of protein), intact glycosomes treated with 20 mM galactose released a higher hexose phosphate concentration to the cytosol in the form of glucose-1-phosphate, when compared to intact glycosomes treated with 20 mM glucose, which predominantly released glucose-6-phosphate. These results shine a light on T. cruzi’s galactose metabolism and its interplay with mechanisms that enable resistance to oxidative stress.
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spelling pubmed-96111772022-10-28 Consumption of Galactose by Trypanosoma cruzi Epimastigotes Generates Resistance against Oxidative Stress Lobo-Rojas, Ángel Quintero-Troconis, Ender Rondón-Mercado, Rocío Pérez-Aguilar., Mary Carmen Concepción, Juan Luis Cáceres, Ana Judith Pathogens Article In this study, we demonstrate that Trypanosoma cruzi epimastigotes previously grown in LIT medium supplemented with 20 mM galactose and exposed to sub-lethal concentrations of hydrogen peroxide (100 μM) showed two-fold and five-fold viability when compared to epimastigotes grown in LIT medium supplemented with two different glucose concentrations (20 mM and 1.5 mM), respectively. Similar results were obtained when exposing epimastigotes from all treatments to methylene blue 30 μM. Additionally, through differential centrifugation and the selective permeabilization of cellular membranes with digitonin, we found that phosphoglucomutase activity (a key enzyme in galactose metabolism) occurs predominantly within the cytosolic compartment. Furthermore, after partially permeabilizing epimastigotes with digitonin (0.025 mg × mg(−1) of protein), intact glycosomes treated with 20 mM galactose released a higher hexose phosphate concentration to the cytosol in the form of glucose-1-phosphate, when compared to intact glycosomes treated with 20 mM glucose, which predominantly released glucose-6-phosphate. These results shine a light on T. cruzi’s galactose metabolism and its interplay with mechanisms that enable resistance to oxidative stress. MDPI 2022-10-11 /pmc/articles/PMC9611177/ /pubmed/36297231 http://dx.doi.org/10.3390/pathogens11101174 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lobo-Rojas, Ángel
Quintero-Troconis, Ender
Rondón-Mercado, Rocío
Pérez-Aguilar., Mary Carmen
Concepción, Juan Luis
Cáceres, Ana Judith
Consumption of Galactose by Trypanosoma cruzi Epimastigotes Generates Resistance against Oxidative Stress
title Consumption of Galactose by Trypanosoma cruzi Epimastigotes Generates Resistance against Oxidative Stress
title_full Consumption of Galactose by Trypanosoma cruzi Epimastigotes Generates Resistance against Oxidative Stress
title_fullStr Consumption of Galactose by Trypanosoma cruzi Epimastigotes Generates Resistance against Oxidative Stress
title_full_unstemmed Consumption of Galactose by Trypanosoma cruzi Epimastigotes Generates Resistance against Oxidative Stress
title_short Consumption of Galactose by Trypanosoma cruzi Epimastigotes Generates Resistance against Oxidative Stress
title_sort consumption of galactose by trypanosoma cruzi epimastigotes generates resistance against oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611177/
https://www.ncbi.nlm.nih.gov/pubmed/36297231
http://dx.doi.org/10.3390/pathogens11101174
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