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The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices

Biomembranes based on an organosilica sol–gel matrix were used to immobilize bacteria Paracoccus yeei VKM B-3302 as part of a biochemical oxygen demand (BOD) biosensor. Diethoxydimethylsilane (DEDMS) and tetraethoxysilane (TEOS) were used as precursors to create the matrix in a 1:1 volume ratio. The...

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Autores principales: Kamanina, Olga A., Lantsova, Elizaveta A., Rybochkin, Pavel V., Arlyapov, Vyacheslav A., Plekhanova, Yulia V., Reshetilov, Anatoly N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611446/
https://www.ncbi.nlm.nih.gov/pubmed/36295743
http://dx.doi.org/10.3390/membranes12100983
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author Kamanina, Olga A.
Lantsova, Elizaveta A.
Rybochkin, Pavel V.
Arlyapov, Vyacheslav A.
Plekhanova, Yulia V.
Reshetilov, Anatoly N.
author_facet Kamanina, Olga A.
Lantsova, Elizaveta A.
Rybochkin, Pavel V.
Arlyapov, Vyacheslav A.
Plekhanova, Yulia V.
Reshetilov, Anatoly N.
author_sort Kamanina, Olga A.
collection PubMed
description Biomembranes based on an organosilica sol–gel matrix were used to immobilize bacteria Paracoccus yeei VKM B-3302 as part of a biochemical oxygen demand (BOD) biosensor. Diethoxydimethylsilane (DEDMS) and tetraethoxysilane (TEOS) were used as precursors to create the matrix in a 1:1 volume ratio. The use of scanning electron microscopy (SEM) and the low-temperature nitrogen adsorption method (BET) showed that the sol–gel matrix forms a capsule around microorganisms that does not prevent the exchange of substrates and waste products of bacteria to the cells. The use of DEDMS as part of the matrix made it possible to increase the sensitivity coefficient of the biosensor for determining BOD by two orders of magnitude compared to a biosensor based on methyltriethoxysilane (MTES). Additionally, the long-term stability of the bioreceptor increased to 68 days. The use of such a matrix neutralized the effect of heavy metal ions on the microorganisms’ catalytic activity in the biosensor. The developed biosensor was used to analyze water samples from water sources in the Tula region (Russia).
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spelling pubmed-96114462022-10-28 The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices Kamanina, Olga A. Lantsova, Elizaveta A. Rybochkin, Pavel V. Arlyapov, Vyacheslav A. Plekhanova, Yulia V. Reshetilov, Anatoly N. Membranes (Basel) Article Biomembranes based on an organosilica sol–gel matrix were used to immobilize bacteria Paracoccus yeei VKM B-3302 as part of a biochemical oxygen demand (BOD) biosensor. Diethoxydimethylsilane (DEDMS) and tetraethoxysilane (TEOS) were used as precursors to create the matrix in a 1:1 volume ratio. The use of scanning electron microscopy (SEM) and the low-temperature nitrogen adsorption method (BET) showed that the sol–gel matrix forms a capsule around microorganisms that does not prevent the exchange of substrates and waste products of bacteria to the cells. The use of DEDMS as part of the matrix made it possible to increase the sensitivity coefficient of the biosensor for determining BOD by two orders of magnitude compared to a biosensor based on methyltriethoxysilane (MTES). Additionally, the long-term stability of the bioreceptor increased to 68 days. The use of such a matrix neutralized the effect of heavy metal ions on the microorganisms’ catalytic activity in the biosensor. The developed biosensor was used to analyze water samples from water sources in the Tula region (Russia). MDPI 2022-10-10 /pmc/articles/PMC9611446/ /pubmed/36295743 http://dx.doi.org/10.3390/membranes12100983 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kamanina, Olga A.
Lantsova, Elizaveta A.
Rybochkin, Pavel V.
Arlyapov, Vyacheslav A.
Plekhanova, Yulia V.
Reshetilov, Anatoly N.
The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices
title The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices
title_full The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices
title_fullStr The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices
title_full_unstemmed The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices
title_short The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices
title_sort use of diethoxydimethylsilane as the basis of a hybrid organosilicon material for the production of biosensitive membranes for sensory devices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611446/
https://www.ncbi.nlm.nih.gov/pubmed/36295743
http://dx.doi.org/10.3390/membranes12100983
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