Cargando…
Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water
The presence of biofilms can negatively affect several different areas, such as the food industry, environment, and biomedical sectors. Conditions under which bacteria grow and develop, such as temperature, nutrients, and pH, among others, can largely influence biofilm production. Staphylococcus spe...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611571/ https://www.ncbi.nlm.nih.gov/pubmed/36297126 http://dx.doi.org/10.3390/pathogens11101069 |
_version_ | 1784819560059240448 |
---|---|
author | Silva, Vanessa Pereira, José Eduardo Maltez, Luís Poeta, Patrícia Igrejas, Gilberto |
author_facet | Silva, Vanessa Pereira, José Eduardo Maltez, Luís Poeta, Patrícia Igrejas, Gilberto |
author_sort | Silva, Vanessa |
collection | PubMed |
description | The presence of biofilms can negatively affect several different areas, such as the food industry, environment, and biomedical sectors. Conditions under which bacteria grow and develop, such as temperature, nutrients, and pH, among others, can largely influence biofilm production. Staphylococcus species survive in the natural environment due to their tolerance to a wide range of temperatures, dryness, dehydration, and low water activity. Therefore, we aimed to evaluate the influence of external environmental factors on the formation of biofilm of staphylococci isolated from hospital wastewater and surface waters. We investigated the biofilm formation of methicillin-resistant and -susceptible S. aureus (MRSA and MSSA) and coagulase-negative staphylococci (CoNS) under various temperatures, pH values, salt concentrations, glucose concentrations, and under anaerobic and aerobic conditions. CoNS had the ability to produce more biofilm biomass than MSSA and MRSA. All environmental factors studied influenced the biofilm formation of staphylococci isolates after 24 h of incubation. Higher biofilm formation was achieved at 4% of NaCl and 0.5% of glucose for MSSA and CoNS, and 1% of NaCl and 1.5% of glucose for MRSA isolates. Biofilm formation of isolates was greater at 25 °C and 37 °C than at 10 °C and 4 °C. pH values between 6 and 8 led to more robust biofilm formation than pH levels of 9 and 5. Although staphylococci are facultative anaerobes, biofilm formation was higher in the presence of oxygen. The results demonstrated that multiple environmental factors affect staphylococci biofilm formation. Different conditions affect differently the biofilm formation of MRSA, MSSA, and CoNS strains. |
format | Online Article Text |
id | pubmed-9611571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96115712022-10-28 Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water Silva, Vanessa Pereira, José Eduardo Maltez, Luís Poeta, Patrícia Igrejas, Gilberto Pathogens Communication The presence of biofilms can negatively affect several different areas, such as the food industry, environment, and biomedical sectors. Conditions under which bacteria grow and develop, such as temperature, nutrients, and pH, among others, can largely influence biofilm production. Staphylococcus species survive in the natural environment due to their tolerance to a wide range of temperatures, dryness, dehydration, and low water activity. Therefore, we aimed to evaluate the influence of external environmental factors on the formation of biofilm of staphylococci isolated from hospital wastewater and surface waters. We investigated the biofilm formation of methicillin-resistant and -susceptible S. aureus (MRSA and MSSA) and coagulase-negative staphylococci (CoNS) under various temperatures, pH values, salt concentrations, glucose concentrations, and under anaerobic and aerobic conditions. CoNS had the ability to produce more biofilm biomass than MSSA and MRSA. All environmental factors studied influenced the biofilm formation of staphylococci isolates after 24 h of incubation. Higher biofilm formation was achieved at 4% of NaCl and 0.5% of glucose for MSSA and CoNS, and 1% of NaCl and 1.5% of glucose for MRSA isolates. Biofilm formation of isolates was greater at 25 °C and 37 °C than at 10 °C and 4 °C. pH values between 6 and 8 led to more robust biofilm formation than pH levels of 9 and 5. Although staphylococci are facultative anaerobes, biofilm formation was higher in the presence of oxygen. The results demonstrated that multiple environmental factors affect staphylococci biofilm formation. Different conditions affect differently the biofilm formation of MRSA, MSSA, and CoNS strains. MDPI 2022-09-20 /pmc/articles/PMC9611571/ /pubmed/36297126 http://dx.doi.org/10.3390/pathogens11101069 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Silva, Vanessa Pereira, José Eduardo Maltez, Luís Poeta, Patrícia Igrejas, Gilberto Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
title | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
title_full | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
title_fullStr | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
title_full_unstemmed | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
title_short | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
title_sort | influence of environmental factors on biofilm formation of staphylococci isolated from wastewater and surface water |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611571/ https://www.ncbi.nlm.nih.gov/pubmed/36297126 http://dx.doi.org/10.3390/pathogens11101069 |
work_keys_str_mv | AT silvavanessa influenceofenvironmentalfactorsonbiofilmformationofstaphylococciisolatedfromwastewaterandsurfacewater AT pereirajoseeduardo influenceofenvironmentalfactorsonbiofilmformationofstaphylococciisolatedfromwastewaterandsurfacewater AT maltezluis influenceofenvironmentalfactorsonbiofilmformationofstaphylococciisolatedfromwastewaterandsurfacewater AT poetapatricia influenceofenvironmentalfactorsonbiofilmformationofstaphylococciisolatedfromwastewaterandsurfacewater AT igrejasgilberto influenceofenvironmentalfactorsonbiofilmformationofstaphylococciisolatedfromwastewaterandsurfacewater |