Cargando…

The Prevalence, Genotype Distribution and Risk Factors of Human Papillomavirus in Tunisia: A National-Based Study

There are limited national population-based studies on HPV genotypes distribution in Tunisia, thus making difficult an assessment of the burden of vaccine-preventable cervical cancer. In this context, we conducted a national survey to determine the HPV prevalence and genotypes distribution and the r...

Descripción completa

Detalles Bibliográficos
Autores principales: Ardhaoui, Monia, Letaief, Hejer, Ennaifer, Emna, Bougatef, Souha, Lassili, Thelja, Bel Haj Rhouma, Rahima, Fehri, Emna, Ouerhani, Kaouther, Guizani, Ikram, Mchela, Myriam, Chahed, Karim, Chahed, Mohamed Kouni, Boubaker, Mohamed Samir, Bouafif Ben Alaya, Nissaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611589/
https://www.ncbi.nlm.nih.gov/pubmed/36298732
http://dx.doi.org/10.3390/v14102175
_version_ 1784819564868009984
author Ardhaoui, Monia
Letaief, Hejer
Ennaifer, Emna
Bougatef, Souha
Lassili, Thelja
Bel Haj Rhouma, Rahima
Fehri, Emna
Ouerhani, Kaouther
Guizani, Ikram
Mchela, Myriam
Chahed, Karim
Chahed, Mohamed Kouni
Boubaker, Mohamed Samir
Bouafif Ben Alaya, Nissaf
author_facet Ardhaoui, Monia
Letaief, Hejer
Ennaifer, Emna
Bougatef, Souha
Lassili, Thelja
Bel Haj Rhouma, Rahima
Fehri, Emna
Ouerhani, Kaouther
Guizani, Ikram
Mchela, Myriam
Chahed, Karim
Chahed, Mohamed Kouni
Boubaker, Mohamed Samir
Bouafif Ben Alaya, Nissaf
author_sort Ardhaoui, Monia
collection PubMed
description There are limited national population-based studies on HPV genotypes distribution in Tunisia, thus making difficult an assessment of the burden of vaccine-preventable cervical cancer. In this context, we conducted a national survey to determine the HPV prevalence and genotypes distribution and the risk factors for HPV infections in Tunisian women. This is a cross-sectional study performed between December 2012 and December 2014. A liquid-based Pap smear sample was obtained from all women and samples’ DNAs were extracted. Only women with betaglobin-positive PCR were further analysed for HPV detection and typing by a nested-PCR of the L1 region followed by next-generation sequencing. A multiple logistic regression model was used for the analysis of associations between the variables. A total of 1517 women were enrolled in this study, and 1229 out of the 1517 cervical samples were positive for the betaglobin control PCR and tested for HPV. Overall HPV infection prevalence was measured to be 7.8% (96/1229), with significant differences between the grand regions, ranging from 2% in the North to 13.1% in Grand Tunis. High-risk HPV genotypes accounted for 5% of the infections. The most prevalent genotypes were HPV 31 (1%), 16 (0.9%), 59 (0.7%). HPV18 was detected only in four cases of the study population. Potential risk factors were living in Grand Tunis region (OR: 7.94 [2.74–22.99]), married status (OR: 2.74 [1.23–6.13]), smoking habit (OR: 2.73 [1.35–5.51]), occupation (OR: 1.81 [1.09–3.01]) and women with multiple sexual partners (OR: 1.91 [1.07–3.39]). These findings underscore the need to evaluate the cost effectiveness of HPV vaccine implementation, contribute to the evidence on the burden of HPV infections, the critical role of sexual behaviour and socioeconomic status, and call for increased support to the preventive program of cervical cancer in Tunisia.
format Online
Article
Text
id pubmed-9611589
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96115892022-10-28 The Prevalence, Genotype Distribution and Risk Factors of Human Papillomavirus in Tunisia: A National-Based Study Ardhaoui, Monia Letaief, Hejer Ennaifer, Emna Bougatef, Souha Lassili, Thelja Bel Haj Rhouma, Rahima Fehri, Emna Ouerhani, Kaouther Guizani, Ikram Mchela, Myriam Chahed, Karim Chahed, Mohamed Kouni Boubaker, Mohamed Samir Bouafif Ben Alaya, Nissaf Viruses Article There are limited national population-based studies on HPV genotypes distribution in Tunisia, thus making difficult an assessment of the burden of vaccine-preventable cervical cancer. In this context, we conducted a national survey to determine the HPV prevalence and genotypes distribution and the risk factors for HPV infections in Tunisian women. This is a cross-sectional study performed between December 2012 and December 2014. A liquid-based Pap smear sample was obtained from all women and samples’ DNAs were extracted. Only women with betaglobin-positive PCR were further analysed for HPV detection and typing by a nested-PCR of the L1 region followed by next-generation sequencing. A multiple logistic regression model was used for the analysis of associations between the variables. A total of 1517 women were enrolled in this study, and 1229 out of the 1517 cervical samples were positive for the betaglobin control PCR and tested for HPV. Overall HPV infection prevalence was measured to be 7.8% (96/1229), with significant differences between the grand regions, ranging from 2% in the North to 13.1% in Grand Tunis. High-risk HPV genotypes accounted for 5% of the infections. The most prevalent genotypes were HPV 31 (1%), 16 (0.9%), 59 (0.7%). HPV18 was detected only in four cases of the study population. Potential risk factors were living in Grand Tunis region (OR: 7.94 [2.74–22.99]), married status (OR: 2.74 [1.23–6.13]), smoking habit (OR: 2.73 [1.35–5.51]), occupation (OR: 1.81 [1.09–3.01]) and women with multiple sexual partners (OR: 1.91 [1.07–3.39]). These findings underscore the need to evaluate the cost effectiveness of HPV vaccine implementation, contribute to the evidence on the burden of HPV infections, the critical role of sexual behaviour and socioeconomic status, and call for increased support to the preventive program of cervical cancer in Tunisia. MDPI 2022-09-30 /pmc/articles/PMC9611589/ /pubmed/36298732 http://dx.doi.org/10.3390/v14102175 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ardhaoui, Monia
Letaief, Hejer
Ennaifer, Emna
Bougatef, Souha
Lassili, Thelja
Bel Haj Rhouma, Rahima
Fehri, Emna
Ouerhani, Kaouther
Guizani, Ikram
Mchela, Myriam
Chahed, Karim
Chahed, Mohamed Kouni
Boubaker, Mohamed Samir
Bouafif Ben Alaya, Nissaf
The Prevalence, Genotype Distribution and Risk Factors of Human Papillomavirus in Tunisia: A National-Based Study
title The Prevalence, Genotype Distribution and Risk Factors of Human Papillomavirus in Tunisia: A National-Based Study
title_full The Prevalence, Genotype Distribution and Risk Factors of Human Papillomavirus in Tunisia: A National-Based Study
title_fullStr The Prevalence, Genotype Distribution and Risk Factors of Human Papillomavirus in Tunisia: A National-Based Study
title_full_unstemmed The Prevalence, Genotype Distribution and Risk Factors of Human Papillomavirus in Tunisia: A National-Based Study
title_short The Prevalence, Genotype Distribution and Risk Factors of Human Papillomavirus in Tunisia: A National-Based Study
title_sort prevalence, genotype distribution and risk factors of human papillomavirus in tunisia: a national-based study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611589/
https://www.ncbi.nlm.nih.gov/pubmed/36298732
http://dx.doi.org/10.3390/v14102175
work_keys_str_mv AT ardhaouimonia theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT letaiefhejer theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT ennaiferemna theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT bougatefsouha theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT lassilithelja theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT belhajrhoumarahima theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT fehriemna theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT ouerhanikaouther theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT guizaniikram theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT mchelamyriam theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT chahedkarim theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT chahedmohamedkouni theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT boubakermohamedsamir theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT bouafifbenalayanissaf theprevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT ardhaouimonia prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT letaiefhejer prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT ennaiferemna prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT bougatefsouha prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT lassilithelja prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT belhajrhoumarahima prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT fehriemna prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT ouerhanikaouther prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT guizaniikram prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT mchelamyriam prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT chahedkarim prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT chahedmohamedkouni prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT boubakermohamedsamir prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy
AT bouafifbenalayanissaf prevalencegenotypedistributionandriskfactorsofhumanpapillomavirusintunisiaanationalbasedstudy