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Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China

Canine parvovirus (CPV) and feline panleukopenia virus (FPV) are highly contagious and cause severe enteric diseases, with high mortality rates in dogs and cats. In the present study, we isolated and identified a novel CPV-2c strain (FPV-DL04 strain) from 18 cats with gastroenteritis symptoms and a...

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Autores principales: Jing, Zheng, Ji, Peng, Wei, Yanquan, Hao, Fuxing, Wei, Yanming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611770/
https://www.ncbi.nlm.nih.gov/pubmed/36311677
http://dx.doi.org/10.3389/fvets.2022.1001604
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author Jing, Zheng
Ji, Peng
Wei, Yanquan
Hao, Fuxing
Wei, Yanming
author_facet Jing, Zheng
Ji, Peng
Wei, Yanquan
Hao, Fuxing
Wei, Yanming
author_sort Jing, Zheng
collection PubMed
description Canine parvovirus (CPV) and feline panleukopenia virus (FPV) are highly contagious and cause severe enteric diseases, with high mortality rates in dogs and cats. In the present study, we isolated and identified a novel CPV-2c strain (FPV-DL04 strain) from 18 cats with gastroenteritis symptoms and a positive parvovirus PCR test result in Dalian, China. Molecular characterization, sequence analysis, and phylogeny determination were performed on the VP2 gene of this strain. The results showed that the FPV-DL04 strain had 99.4% homology with the CPV-2c CN/HN1708 strain, and both strains had S297A and A300G key mutation sites. Interestingly, we also found that the DL04 strain has a A5G mutation site, but no F267Y and Y324I mutation sites. This study provided new important findings regarding the evolution of parvovirus infection in domestic cats in China.
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spelling pubmed-96117702022-10-28 Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China Jing, Zheng Ji, Peng Wei, Yanquan Hao, Fuxing Wei, Yanming Front Vet Sci Veterinary Science Canine parvovirus (CPV) and feline panleukopenia virus (FPV) are highly contagious and cause severe enteric diseases, with high mortality rates in dogs and cats. In the present study, we isolated and identified a novel CPV-2c strain (FPV-DL04 strain) from 18 cats with gastroenteritis symptoms and a positive parvovirus PCR test result in Dalian, China. Molecular characterization, sequence analysis, and phylogeny determination were performed on the VP2 gene of this strain. The results showed that the FPV-DL04 strain had 99.4% homology with the CPV-2c CN/HN1708 strain, and both strains had S297A and A300G key mutation sites. Interestingly, we also found that the DL04 strain has a A5G mutation site, but no F267Y and Y324I mutation sites. This study provided new important findings regarding the evolution of parvovirus infection in domestic cats in China. Frontiers Media S.A. 2022-10-13 /pmc/articles/PMC9611770/ /pubmed/36311677 http://dx.doi.org/10.3389/fvets.2022.1001604 Text en Copyright © 2022 Jing, Ji, Wei, Hao and Wei. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Jing, Zheng
Ji, Peng
Wei, Yanquan
Hao, Fuxing
Wei, Yanming
Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China
title Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China
title_full Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China
title_fullStr Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China
title_full_unstemmed Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China
title_short Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China
title_sort isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in dalian, china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611770/
https://www.ncbi.nlm.nih.gov/pubmed/36311677
http://dx.doi.org/10.3389/fvets.2022.1001604
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