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Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China
Canine parvovirus (CPV) and feline panleukopenia virus (FPV) are highly contagious and cause severe enteric diseases, with high mortality rates in dogs and cats. In the present study, we isolated and identified a novel CPV-2c strain (FPV-DL04 strain) from 18 cats with gastroenteritis symptoms and a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611770/ https://www.ncbi.nlm.nih.gov/pubmed/36311677 http://dx.doi.org/10.3389/fvets.2022.1001604 |
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author | Jing, Zheng Ji, Peng Wei, Yanquan Hao, Fuxing Wei, Yanming |
author_facet | Jing, Zheng Ji, Peng Wei, Yanquan Hao, Fuxing Wei, Yanming |
author_sort | Jing, Zheng |
collection | PubMed |
description | Canine parvovirus (CPV) and feline panleukopenia virus (FPV) are highly contagious and cause severe enteric diseases, with high mortality rates in dogs and cats. In the present study, we isolated and identified a novel CPV-2c strain (FPV-DL04 strain) from 18 cats with gastroenteritis symptoms and a positive parvovirus PCR test result in Dalian, China. Molecular characterization, sequence analysis, and phylogeny determination were performed on the VP2 gene of this strain. The results showed that the FPV-DL04 strain had 99.4% homology with the CPV-2c CN/HN1708 strain, and both strains had S297A and A300G key mutation sites. Interestingly, we also found that the DL04 strain has a A5G mutation site, but no F267Y and Y324I mutation sites. This study provided new important findings regarding the evolution of parvovirus infection in domestic cats in China. |
format | Online Article Text |
id | pubmed-9611770 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96117702022-10-28 Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China Jing, Zheng Ji, Peng Wei, Yanquan Hao, Fuxing Wei, Yanming Front Vet Sci Veterinary Science Canine parvovirus (CPV) and feline panleukopenia virus (FPV) are highly contagious and cause severe enteric diseases, with high mortality rates in dogs and cats. In the present study, we isolated and identified a novel CPV-2c strain (FPV-DL04 strain) from 18 cats with gastroenteritis symptoms and a positive parvovirus PCR test result in Dalian, China. Molecular characterization, sequence analysis, and phylogeny determination were performed on the VP2 gene of this strain. The results showed that the FPV-DL04 strain had 99.4% homology with the CPV-2c CN/HN1708 strain, and both strains had S297A and A300G key mutation sites. Interestingly, we also found that the DL04 strain has a A5G mutation site, but no F267Y and Y324I mutation sites. This study provided new important findings regarding the evolution of parvovirus infection in domestic cats in China. Frontiers Media S.A. 2022-10-13 /pmc/articles/PMC9611770/ /pubmed/36311677 http://dx.doi.org/10.3389/fvets.2022.1001604 Text en Copyright © 2022 Jing, Ji, Wei, Hao and Wei. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Jing, Zheng Ji, Peng Wei, Yanquan Hao, Fuxing Wei, Yanming Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China |
title | Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China |
title_full | Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China |
title_fullStr | Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China |
title_full_unstemmed | Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China |
title_short | Isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in Dalian, China |
title_sort | isolation and identification of a novel canine parvovirus type 2c strain in domestic cats in dalian, china |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611770/ https://www.ncbi.nlm.nih.gov/pubmed/36311677 http://dx.doi.org/10.3389/fvets.2022.1001604 |
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