T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device

Immune surveillance is dependent on lymphocyte migration and targeted recruitment. This can involve different modes of cell motility ranging from random walk to highly directional environment-guided migration driven by chemotaxis. This study protocol describes a flow-based microfluidic device to per...

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Detalles Bibliográficos
Autores principales: Liu, Yang, Ren, Xiaoou, Wu, Jiandong, Wilkins, John A., Lin, Francis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Study Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611841/
https://www.ncbi.nlm.nih.gov/pubmed/36295920
http://dx.doi.org/10.3390/mi13101567
Descripción
Sumario:Immune surveillance is dependent on lymphocyte migration and targeted recruitment. This can involve different modes of cell motility ranging from random walk to highly directional environment-guided migration driven by chemotaxis. This study protocol describes a flow-based microfluidic device to perform quantitative multiplex cell migration assays with the potential to investigate in real time the migratory response of T cells at the population or single-cell level. The device also allows for subsequent in situ fixation and direct fluorescence analysis of the cells in the microchannel.

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