T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device

Immune surveillance is dependent on lymphocyte migration and targeted recruitment. This can involve different modes of cell motility ranging from random walk to highly directional environment-guided migration driven by chemotaxis. This study protocol describes a flow-based microfluidic device to per...

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Detalles Bibliográficos
Autores principales: Liu, Yang, Ren, Xiaoou, Wu, Jiandong, Wilkins, John A., Lin, Francis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Study Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611841/
https://www.ncbi.nlm.nih.gov/pubmed/36295920
http://dx.doi.org/10.3390/mi13101567
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author Liu, Yang
Ren, Xiaoou
Wu, Jiandong
Wilkins, John A.
Lin, Francis
author_facet Liu, Yang
Ren, Xiaoou
Wu, Jiandong
Wilkins, John A.
Lin, Francis
author_sort Liu, Yang
collection PubMed
description Immune surveillance is dependent on lymphocyte migration and targeted recruitment. This can involve different modes of cell motility ranging from random walk to highly directional environment-guided migration driven by chemotaxis. This study protocol describes a flow-based microfluidic device to perform quantitative multiplex cell migration assays with the potential to investigate in real time the migratory response of T cells at the population or single-cell level. The device also allows for subsequent in situ fixation and direct fluorescence analysis of the cells in the microchannel.
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spelling pubmed-96118412022-10-28 T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device Liu, Yang Ren, Xiaoou Wu, Jiandong Wilkins, John A. Lin, Francis Micromachines (Basel) Study Protocol Immune surveillance is dependent on lymphocyte migration and targeted recruitment. This can involve different modes of cell motility ranging from random walk to highly directional environment-guided migration driven by chemotaxis. This study protocol describes a flow-based microfluidic device to perform quantitative multiplex cell migration assays with the potential to investigate in real time the migratory response of T cells at the population or single-cell level. The device also allows for subsequent in situ fixation and direct fluorescence analysis of the cells in the microchannel. MDPI 2022-09-21 /pmc/articles/PMC9611841/ /pubmed/36295920 http://dx.doi.org/10.3390/mi13101567 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Study Protocol
Liu, Yang
Ren, Xiaoou
Wu, Jiandong
Wilkins, John A.
Lin, Francis
T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device
title T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device
title_full T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device
title_fullStr T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device
title_full_unstemmed T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device
title_short T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device
title_sort t cells chemotaxis migration studies with a multi-channel microfluidic device
topic Study Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611841/
https://www.ncbi.nlm.nih.gov/pubmed/36295920
http://dx.doi.org/10.3390/mi13101567
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AT renxiaoou tcellschemotaxismigrationstudieswithamultichannelmicrofluidicdevice
AT wujiandong tcellschemotaxismigrationstudieswithamultichannelmicrofluidicdevice
AT wilkinsjohna tcellschemotaxismigrationstudieswithamultichannelmicrofluidicdevice
AT linfrancis tcellschemotaxismigrationstudieswithamultichannelmicrofluidicdevice

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