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Differential Metabolism of Glycerol Based on Oral versus Intravenous Administration in Humans

Glycerol can be metabolized to glucose via gluconeogenesis or lactate via glycolysis. It is unknown if glycerol is metabolized similarly in the portal and systemic circulations in humans. Eight metabolically healthy overnight-fasted individuals received equimolar amounts of (13)C(3)-glycerol orally...

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Autores principales: Shah, Ankit, Wang, Yujue, Wondisford, Fredric E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611849/
https://www.ncbi.nlm.nih.gov/pubmed/36295792
http://dx.doi.org/10.3390/metabo12100890
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author Shah, Ankit
Wang, Yujue
Wondisford, Fredric E.
author_facet Shah, Ankit
Wang, Yujue
Wondisford, Fredric E.
author_sort Shah, Ankit
collection PubMed
description Glycerol can be metabolized to glucose via gluconeogenesis or lactate via glycolysis. It is unknown if glycerol is metabolized similarly in the portal and systemic circulations in humans. Eight metabolically healthy overnight-fasted individuals received equimolar amounts of (13)C(3)-glycerol orally and intravenously on two separate occasions with serial blood draws over four hours. Serum samples underwent liquid chromatography–mass spectrometry analysis. Oral (13)C(3)-glycerol administration led to higher average serum glucose enrichment than intravenous administration (5.02 ± 1.43 versus 4.07 ± 0.79%, p = 0.009). In contrast, intravenous (13)C(3)-glycerol administration yielded higher average serum lactate enrichment than oral administration (5.67 ± 0.80 versus 4.85 ± 1.30%, p = 0.032). Peak serum glucose enrichment was also higher with oral administration (9.37 ± 2.93 versus 7.12 ± 1.28%, p = 0.010). Glycerol metabolism across the portal and systemic circulations is not congruent. Orally administered labeled glycerol led to greater labeled glucose production, while intravenously administration yielded greater lactate production. These data support direct glycerol to lactate conversion in humans.
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spelling pubmed-96118492022-10-28 Differential Metabolism of Glycerol Based on Oral versus Intravenous Administration in Humans Shah, Ankit Wang, Yujue Wondisford, Fredric E. Metabolites Article Glycerol can be metabolized to glucose via gluconeogenesis or lactate via glycolysis. It is unknown if glycerol is metabolized similarly in the portal and systemic circulations in humans. Eight metabolically healthy overnight-fasted individuals received equimolar amounts of (13)C(3)-glycerol orally and intravenously on two separate occasions with serial blood draws over four hours. Serum samples underwent liquid chromatography–mass spectrometry analysis. Oral (13)C(3)-glycerol administration led to higher average serum glucose enrichment than intravenous administration (5.02 ± 1.43 versus 4.07 ± 0.79%, p = 0.009). In contrast, intravenous (13)C(3)-glycerol administration yielded higher average serum lactate enrichment than oral administration (5.67 ± 0.80 versus 4.85 ± 1.30%, p = 0.032). Peak serum glucose enrichment was also higher with oral administration (9.37 ± 2.93 versus 7.12 ± 1.28%, p = 0.010). Glycerol metabolism across the portal and systemic circulations is not congruent. Orally administered labeled glycerol led to greater labeled glucose production, while intravenously administration yielded greater lactate production. These data support direct glycerol to lactate conversion in humans. MDPI 2022-09-22 /pmc/articles/PMC9611849/ /pubmed/36295792 http://dx.doi.org/10.3390/metabo12100890 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shah, Ankit
Wang, Yujue
Wondisford, Fredric E.
Differential Metabolism of Glycerol Based on Oral versus Intravenous Administration in Humans
title Differential Metabolism of Glycerol Based on Oral versus Intravenous Administration in Humans
title_full Differential Metabolism of Glycerol Based on Oral versus Intravenous Administration in Humans
title_fullStr Differential Metabolism of Glycerol Based on Oral versus Intravenous Administration in Humans
title_full_unstemmed Differential Metabolism of Glycerol Based on Oral versus Intravenous Administration in Humans
title_short Differential Metabolism of Glycerol Based on Oral versus Intravenous Administration in Humans
title_sort differential metabolism of glycerol based on oral versus intravenous administration in humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611849/
https://www.ncbi.nlm.nih.gov/pubmed/36295792
http://dx.doi.org/10.3390/metabo12100890
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