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Comparison of Hepatic Metabolite Profiles between Infant and Adult Male Mice Using (1)H-NMR-Based Untargeted Metabolomics
Although age-related characteristics of hepatic metabolism are reported, those in infants are not fully understood. In the present study, we performed untargeted metabolomic profiling of the livers of infant (3-week-old) and adult (9-week-old) male ICR mice using (1)H-NMR spectroscopy and compared 3...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611911/ https://www.ncbi.nlm.nih.gov/pubmed/36295812 http://dx.doi.org/10.3390/metabo12100910 |
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author | Kwon, Doyoung Lee, Wonho Kim, Sou Hyun Jung, Young-Suk |
author_facet | Kwon, Doyoung Lee, Wonho Kim, Sou Hyun Jung, Young-Suk |
author_sort | Kwon, Doyoung |
collection | PubMed |
description | Although age-related characteristics of hepatic metabolism are reported, those in infants are not fully understood. In the present study, we performed untargeted metabolomic profiling of the livers of infant (3-week-old) and adult (9-week-old) male ICR mice using (1)H-NMR spectroscopy and compared 35 abundant hepatic metabolite concentrations between the two groups. The liver/body weight ratio did not differ between the two groups; however, serum glucose, blood urea nitrogen, total cholesterol, and triglyceride concentrations were lower in infants than in adults. Hepatic carbohydrate metabolites (glucose, maltose, and mannose) were higher, whereas amino acids (glutamine, leucine, methionine, phenylalanine, tyrosine, and valine) were lower in infant mice than in adult mice. The concentrations of ascorbate, betaine, sarcosine, and ethanolamine were higher, whereas those of taurine, inosine, and O-phosphocholine were lower in infant mice than in adult mice. The differences in liver metabolites between the two groups could be due to differences in their developmental stages and dietary sources (breast milk for infants and laboratory chow for adults). The above results provide insights into the hepatic metabolism in infants; however, the exact implications of the findings require further investigation. |
format | Online Article Text |
id | pubmed-9611911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96119112022-10-28 Comparison of Hepatic Metabolite Profiles between Infant and Adult Male Mice Using (1)H-NMR-Based Untargeted Metabolomics Kwon, Doyoung Lee, Wonho Kim, Sou Hyun Jung, Young-Suk Metabolites Article Although age-related characteristics of hepatic metabolism are reported, those in infants are not fully understood. In the present study, we performed untargeted metabolomic profiling of the livers of infant (3-week-old) and adult (9-week-old) male ICR mice using (1)H-NMR spectroscopy and compared 35 abundant hepatic metabolite concentrations between the two groups. The liver/body weight ratio did not differ between the two groups; however, serum glucose, blood urea nitrogen, total cholesterol, and triglyceride concentrations were lower in infants than in adults. Hepatic carbohydrate metabolites (glucose, maltose, and mannose) were higher, whereas amino acids (glutamine, leucine, methionine, phenylalanine, tyrosine, and valine) were lower in infant mice than in adult mice. The concentrations of ascorbate, betaine, sarcosine, and ethanolamine were higher, whereas those of taurine, inosine, and O-phosphocholine were lower in infant mice than in adult mice. The differences in liver metabolites between the two groups could be due to differences in their developmental stages and dietary sources (breast milk for infants and laboratory chow for adults). The above results provide insights into the hepatic metabolism in infants; however, the exact implications of the findings require further investigation. MDPI 2022-09-27 /pmc/articles/PMC9611911/ /pubmed/36295812 http://dx.doi.org/10.3390/metabo12100910 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kwon, Doyoung Lee, Wonho Kim, Sou Hyun Jung, Young-Suk Comparison of Hepatic Metabolite Profiles between Infant and Adult Male Mice Using (1)H-NMR-Based Untargeted Metabolomics |
title | Comparison of Hepatic Metabolite Profiles between Infant and Adult Male Mice Using (1)H-NMR-Based Untargeted Metabolomics |
title_full | Comparison of Hepatic Metabolite Profiles between Infant and Adult Male Mice Using (1)H-NMR-Based Untargeted Metabolomics |
title_fullStr | Comparison of Hepatic Metabolite Profiles between Infant and Adult Male Mice Using (1)H-NMR-Based Untargeted Metabolomics |
title_full_unstemmed | Comparison of Hepatic Metabolite Profiles between Infant and Adult Male Mice Using (1)H-NMR-Based Untargeted Metabolomics |
title_short | Comparison of Hepatic Metabolite Profiles between Infant and Adult Male Mice Using (1)H-NMR-Based Untargeted Metabolomics |
title_sort | comparison of hepatic metabolite profiles between infant and adult male mice using (1)h-nmr-based untargeted metabolomics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9611911/ https://www.ncbi.nlm.nih.gov/pubmed/36295812 http://dx.doi.org/10.3390/metabo12100910 |
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