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Contact Lens Wear Induces Alterations of Lactoferrin Functionality in Human Tears
The tear film is a complex matrix composed of several molecular classes, from small metal ions to macromolecules. Contact lens (CL) wear can affect the protein homeostasis of the tear film, by accumulating deposits on the CL surface and/or altering their structural and functional properties. This wo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612143/ https://www.ncbi.nlm.nih.gov/pubmed/36297623 http://dx.doi.org/10.3390/pharmaceutics14102188 |
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author | Ponzini, Erika Tavazzi, Silvia Musile, Giacomo Tagliaro, Franco Grandori, Rita Santambrogio, Carlo |
author_facet | Ponzini, Erika Tavazzi, Silvia Musile, Giacomo Tagliaro, Franco Grandori, Rita Santambrogio, Carlo |
author_sort | Ponzini, Erika |
collection | PubMed |
description | The tear film is a complex matrix composed of several molecular classes, from small metal ions to macromolecules. Contact lens (CL) wear can affect the protein homeostasis of the tear film, by accumulating deposits on the CL surface and/or altering their structural and functional properties. This work investigates the effect of CL wear on lactoferrin (Lf), one of the most abundant tear proteins, known as an unspecific biomarker of inflammation. Tears from eight volunteers were collected and analyzed after alternated periods of CL wear and without CL. The experimental approach is to probe Lf into unprocessed human tears by the peculiar fluorescence emission originating from complex formation of Lf with terbium (Tb(3+)) at the iron-binding sites. The experimental data indicate that CL wear does not significantly affect the total amount of Lf. On the other hand, Lf affinity for Tb(3+) is reduced upon CL wear, suggesting relevant changes in Lf structure and possible alterations of protein functionality. Future studies based on this approach will help define CL features (material, lens-care solution, wearing time, etc.) with minimal effects on tear protein activity, in order to obtain more biocompatible and comfortable devices. |
format | Online Article Text |
id | pubmed-9612143 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96121432022-10-28 Contact Lens Wear Induces Alterations of Lactoferrin Functionality in Human Tears Ponzini, Erika Tavazzi, Silvia Musile, Giacomo Tagliaro, Franco Grandori, Rita Santambrogio, Carlo Pharmaceutics Communication The tear film is a complex matrix composed of several molecular classes, from small metal ions to macromolecules. Contact lens (CL) wear can affect the protein homeostasis of the tear film, by accumulating deposits on the CL surface and/or altering their structural and functional properties. This work investigates the effect of CL wear on lactoferrin (Lf), one of the most abundant tear proteins, known as an unspecific biomarker of inflammation. Tears from eight volunteers were collected and analyzed after alternated periods of CL wear and without CL. The experimental approach is to probe Lf into unprocessed human tears by the peculiar fluorescence emission originating from complex formation of Lf with terbium (Tb(3+)) at the iron-binding sites. The experimental data indicate that CL wear does not significantly affect the total amount of Lf. On the other hand, Lf affinity for Tb(3+) is reduced upon CL wear, suggesting relevant changes in Lf structure and possible alterations of protein functionality. Future studies based on this approach will help define CL features (material, lens-care solution, wearing time, etc.) with minimal effects on tear protein activity, in order to obtain more biocompatible and comfortable devices. MDPI 2022-10-14 /pmc/articles/PMC9612143/ /pubmed/36297623 http://dx.doi.org/10.3390/pharmaceutics14102188 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Ponzini, Erika Tavazzi, Silvia Musile, Giacomo Tagliaro, Franco Grandori, Rita Santambrogio, Carlo Contact Lens Wear Induces Alterations of Lactoferrin Functionality in Human Tears |
title | Contact Lens Wear Induces Alterations of Lactoferrin Functionality in Human Tears |
title_full | Contact Lens Wear Induces Alterations of Lactoferrin Functionality in Human Tears |
title_fullStr | Contact Lens Wear Induces Alterations of Lactoferrin Functionality in Human Tears |
title_full_unstemmed | Contact Lens Wear Induces Alterations of Lactoferrin Functionality in Human Tears |
title_short | Contact Lens Wear Induces Alterations of Lactoferrin Functionality in Human Tears |
title_sort | contact lens wear induces alterations of lactoferrin functionality in human tears |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612143/ https://www.ncbi.nlm.nih.gov/pubmed/36297623 http://dx.doi.org/10.3390/pharmaceutics14102188 |
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