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Interspecies Recombination-Led Speciation of a Novel Geminivirus in Pakistan

Recombination between isolates of different virus species has been known to be one of the sources of speciation. Weeds serve as mixing vessels for begomoviruses, infecting a wide range of economically important plants, thereby facilitating recombination. Chenopodium album is an economically importan...

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Autores principales: Lal, Aamir, Kil, Eui-Joon, Vo, Thuy T. B., Wira Sanjaya, I Gusti Ngurah Prabu, Qureshi, Muhammad Amir, Nattanong, Bupi, Ali, Muhammad, Shuja, Malik Nawaz, Lee, Sukchan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612148/
https://www.ncbi.nlm.nih.gov/pubmed/36298721
http://dx.doi.org/10.3390/v14102166
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author Lal, Aamir
Kil, Eui-Joon
Vo, Thuy T. B.
Wira Sanjaya, I Gusti Ngurah Prabu
Qureshi, Muhammad Amir
Nattanong, Bupi
Ali, Muhammad
Shuja, Malik Nawaz
Lee, Sukchan
author_facet Lal, Aamir
Kil, Eui-Joon
Vo, Thuy T. B.
Wira Sanjaya, I Gusti Ngurah Prabu
Qureshi, Muhammad Amir
Nattanong, Bupi
Ali, Muhammad
Shuja, Malik Nawaz
Lee, Sukchan
author_sort Lal, Aamir
collection PubMed
description Recombination between isolates of different virus species has been known to be one of the sources of speciation. Weeds serve as mixing vessels for begomoviruses, infecting a wide range of economically important plants, thereby facilitating recombination. Chenopodium album is an economically important weed spread worldwide. Here, we present the molecular characterization of a novel recombinant begomovirus identified from C. album in Lahore, Pakistan. The complete DNA- A genome of the virus associated with the leaf distortion occurred in the infected C. album plants was cloned and sequenced. DNA sequence analysis showed that the nucleotide sequence of the virus shared 93% identity with those of the rose leaf curl virus and the duranta leaf curl virus. Interestingly, this newly identified virus is composed of open reading frames (ORFs) from different origins. Phylogenetic networks and complementary recombination detection methods revealed extensive recombination among the sequences. The infectious clone of the newly detected virus was found to be fully infectious in C. album and Nicotiana benthamiana as the viral DNA was successfully reconstituted from systemically infected tissues of inoculated plants, thus fulfilling Koch’s postulates. Our study reveals a new speciation of an emergent ssDNA plant virus associated with C. album through recombination and therefore, proposed the tentative name ‘Chenopodium leaf distortion virus’ (CLDV).
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spelling pubmed-96121482022-10-28 Interspecies Recombination-Led Speciation of a Novel Geminivirus in Pakistan Lal, Aamir Kil, Eui-Joon Vo, Thuy T. B. Wira Sanjaya, I Gusti Ngurah Prabu Qureshi, Muhammad Amir Nattanong, Bupi Ali, Muhammad Shuja, Malik Nawaz Lee, Sukchan Viruses Article Recombination between isolates of different virus species has been known to be one of the sources of speciation. Weeds serve as mixing vessels for begomoviruses, infecting a wide range of economically important plants, thereby facilitating recombination. Chenopodium album is an economically important weed spread worldwide. Here, we present the molecular characterization of a novel recombinant begomovirus identified from C. album in Lahore, Pakistan. The complete DNA- A genome of the virus associated with the leaf distortion occurred in the infected C. album plants was cloned and sequenced. DNA sequence analysis showed that the nucleotide sequence of the virus shared 93% identity with those of the rose leaf curl virus and the duranta leaf curl virus. Interestingly, this newly identified virus is composed of open reading frames (ORFs) from different origins. Phylogenetic networks and complementary recombination detection methods revealed extensive recombination among the sequences. The infectious clone of the newly detected virus was found to be fully infectious in C. album and Nicotiana benthamiana as the viral DNA was successfully reconstituted from systemically infected tissues of inoculated plants, thus fulfilling Koch’s postulates. Our study reveals a new speciation of an emergent ssDNA plant virus associated with C. album through recombination and therefore, proposed the tentative name ‘Chenopodium leaf distortion virus’ (CLDV). MDPI 2022-09-30 /pmc/articles/PMC9612148/ /pubmed/36298721 http://dx.doi.org/10.3390/v14102166 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lal, Aamir
Kil, Eui-Joon
Vo, Thuy T. B.
Wira Sanjaya, I Gusti Ngurah Prabu
Qureshi, Muhammad Amir
Nattanong, Bupi
Ali, Muhammad
Shuja, Malik Nawaz
Lee, Sukchan
Interspecies Recombination-Led Speciation of a Novel Geminivirus in Pakistan
title Interspecies Recombination-Led Speciation of a Novel Geminivirus in Pakistan
title_full Interspecies Recombination-Led Speciation of a Novel Geminivirus in Pakistan
title_fullStr Interspecies Recombination-Led Speciation of a Novel Geminivirus in Pakistan
title_full_unstemmed Interspecies Recombination-Led Speciation of a Novel Geminivirus in Pakistan
title_short Interspecies Recombination-Led Speciation of a Novel Geminivirus in Pakistan
title_sort interspecies recombination-led speciation of a novel geminivirus in pakistan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612148/
https://www.ncbi.nlm.nih.gov/pubmed/36298721
http://dx.doi.org/10.3390/v14102166
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