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First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines
Goats are key livestock animals and goat raising is an income-generating venture for smallholder farmers, supporting agricultural development in many parts of the world. However, goat production is often limited by various factors, such as tick-borne diseases. Goat piroplasmosis is a disease caused...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612162/ https://www.ncbi.nlm.nih.gov/pubmed/36297166 http://dx.doi.org/10.3390/pathogens11101109 |
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author | Galon, Eloiza May Ybañez, Rochelle Haidee Macalanda, Adrian Miki Estabillo, Giemelene Rose Montano, Margaret Therese Rose Veedor, Marielle Danise Garvida, Anatolio Fabon, Ralph Joselle Callanta, Mary Ruth Labutong, Kim Joseph Tumwebaze, Maria Agnes Byamukama, Benedicto Ji, Shengwei Zafar, Iqra Ybañez, Adrian Xuan, Xuenan |
author_facet | Galon, Eloiza May Ybañez, Rochelle Haidee Macalanda, Adrian Miki Estabillo, Giemelene Rose Montano, Margaret Therese Rose Veedor, Marielle Danise Garvida, Anatolio Fabon, Ralph Joselle Callanta, Mary Ruth Labutong, Kim Joseph Tumwebaze, Maria Agnes Byamukama, Benedicto Ji, Shengwei Zafar, Iqra Ybañez, Adrian Xuan, Xuenan |
author_sort | Galon, Eloiza May |
collection | PubMed |
description | Goats are key livestock animals and goat raising is an income-generating venture for smallholder farmers, supporting agricultural development in many parts of the world. However, goat production is often limited by various factors, such as tick-borne diseases. Goat piroplasmosis is a disease caused by apicomplexan parasites Babesia spp. and Theileria spp., while anaplasmosis is caused by bacterial Anaplasma spp. In the Philippines, the presence of Babesia, Theileria, and Anaplasma has not been reported in goats. In this study, DNA obtained from goats were molecularly screened for Babesia/Theileria and Anaplasma. Of 396, 77.02% (305/396) and 38.64% (153/396) were positive for piroplasma and Anaplasma using PCR assays targeting the 18S rRNA and 16S rRNA genes, respectively. Similarly, Babesia ovis was detected in six samples (1.52%). Representative Babesia/Theileria sequences shared 89.97–97.74% identity with each other and were most closely related to T. orientalis, T. annulata, and Theileria spp. Meanwhile, Anaplasma 16SrRNA sequences were related to A. odocoilei, A. platys, and A. phagocytophilum. This is the first molecular identification of B. ovis, Theileria spp., and Anaplasma spp. in goats from the Philippines. |
format | Online Article Text |
id | pubmed-9612162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96121622022-10-28 First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines Galon, Eloiza May Ybañez, Rochelle Haidee Macalanda, Adrian Miki Estabillo, Giemelene Rose Montano, Margaret Therese Rose Veedor, Marielle Danise Garvida, Anatolio Fabon, Ralph Joselle Callanta, Mary Ruth Labutong, Kim Joseph Tumwebaze, Maria Agnes Byamukama, Benedicto Ji, Shengwei Zafar, Iqra Ybañez, Adrian Xuan, Xuenan Pathogens Communication Goats are key livestock animals and goat raising is an income-generating venture for smallholder farmers, supporting agricultural development in many parts of the world. However, goat production is often limited by various factors, such as tick-borne diseases. Goat piroplasmosis is a disease caused by apicomplexan parasites Babesia spp. and Theileria spp., while anaplasmosis is caused by bacterial Anaplasma spp. In the Philippines, the presence of Babesia, Theileria, and Anaplasma has not been reported in goats. In this study, DNA obtained from goats were molecularly screened for Babesia/Theileria and Anaplasma. Of 396, 77.02% (305/396) and 38.64% (153/396) were positive for piroplasma and Anaplasma using PCR assays targeting the 18S rRNA and 16S rRNA genes, respectively. Similarly, Babesia ovis was detected in six samples (1.52%). Representative Babesia/Theileria sequences shared 89.97–97.74% identity with each other and were most closely related to T. orientalis, T. annulata, and Theileria spp. Meanwhile, Anaplasma 16SrRNA sequences were related to A. odocoilei, A. platys, and A. phagocytophilum. This is the first molecular identification of B. ovis, Theileria spp., and Anaplasma spp. in goats from the Philippines. MDPI 2022-09-27 /pmc/articles/PMC9612162/ /pubmed/36297166 http://dx.doi.org/10.3390/pathogens11101109 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Galon, Eloiza May Ybañez, Rochelle Haidee Macalanda, Adrian Miki Estabillo, Giemelene Rose Montano, Margaret Therese Rose Veedor, Marielle Danise Garvida, Anatolio Fabon, Ralph Joselle Callanta, Mary Ruth Labutong, Kim Joseph Tumwebaze, Maria Agnes Byamukama, Benedicto Ji, Shengwei Zafar, Iqra Ybañez, Adrian Xuan, Xuenan First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines |
title | First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines |
title_full | First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines |
title_fullStr | First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines |
title_full_unstemmed | First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines |
title_short | First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines |
title_sort | first molecular identification of babesia, theileria, and anaplasma in goats from the philippines |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612162/ https://www.ncbi.nlm.nih.gov/pubmed/36297166 http://dx.doi.org/10.3390/pathogens11101109 |
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