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Garlic Potyviruses Are Translocated to the True Seeds through the Vegetative and Reproductive Systems of the Mother Plant

Garlic lost its ability to produce true seeds millennia ago, and today non-fertile commercial cultivars are propagated only vegetatively. Garlic viruses are commonly carried over from one generation of vegetative propagules to the other, while nematodes and arthropods further transmit the pathogens...

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Autores principales: Shemesh-Mayer, Einat, Gelbart, Dana, Belausov, Eduard, Sher, Nisan, Daus, Ahuva, Rabinowitch, Haim D., Kamenetsky-Goldstein, Rina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612218/
https://www.ncbi.nlm.nih.gov/pubmed/36298648
http://dx.doi.org/10.3390/v14102092
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author Shemesh-Mayer, Einat
Gelbart, Dana
Belausov, Eduard
Sher, Nisan
Daus, Ahuva
Rabinowitch, Haim D.
Kamenetsky-Goldstein, Rina
author_facet Shemesh-Mayer, Einat
Gelbart, Dana
Belausov, Eduard
Sher, Nisan
Daus, Ahuva
Rabinowitch, Haim D.
Kamenetsky-Goldstein, Rina
author_sort Shemesh-Mayer, Einat
collection PubMed
description Garlic lost its ability to produce true seeds millennia ago, and today non-fertile commercial cultivars are propagated only vegetatively. Garlic viruses are commonly carried over from one generation of vegetative propagules to the other, while nematodes and arthropods further transmit the pathogens from infected to healthy plants. A recent breakthrough in the production of true (botanical) garlic seeds resulted in rapid scientific progress, but the question of whether viruses are transmitted via seeds remains open and is important for the further development of commercial seed production. We combined morpho-physiological analysis, fluorescence in situ hybridization (FISH), and PCR analysis to follow potyvirus localization and translocation within garlic fertile plants and seeds. Spatial distribution was recorded in both vegetative and reproductive organs. We conclude that garlic potyviruses are translocated to the seeds from the infected mother plant during flower development and post-fertilization, while pollen remains virus-free and does not contribute to seed infection. Therefore, the main practical goal for virus-clean seed production in garlic is the careful maintenance of virus-free mother plants. Although garlic pollen is free of potyviral infection, the male parents’ plants also need to be protected from contamination, since viral infection weakens plants, reducing flowering ability and pollen production.
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spelling pubmed-96122182022-10-28 Garlic Potyviruses Are Translocated to the True Seeds through the Vegetative and Reproductive Systems of the Mother Plant Shemesh-Mayer, Einat Gelbart, Dana Belausov, Eduard Sher, Nisan Daus, Ahuva Rabinowitch, Haim D. Kamenetsky-Goldstein, Rina Viruses Article Garlic lost its ability to produce true seeds millennia ago, and today non-fertile commercial cultivars are propagated only vegetatively. Garlic viruses are commonly carried over from one generation of vegetative propagules to the other, while nematodes and arthropods further transmit the pathogens from infected to healthy plants. A recent breakthrough in the production of true (botanical) garlic seeds resulted in rapid scientific progress, but the question of whether viruses are transmitted via seeds remains open and is important for the further development of commercial seed production. We combined morpho-physiological analysis, fluorescence in situ hybridization (FISH), and PCR analysis to follow potyvirus localization and translocation within garlic fertile plants and seeds. Spatial distribution was recorded in both vegetative and reproductive organs. We conclude that garlic potyviruses are translocated to the seeds from the infected mother plant during flower development and post-fertilization, while pollen remains virus-free and does not contribute to seed infection. Therefore, the main practical goal for virus-clean seed production in garlic is the careful maintenance of virus-free mother plants. Although garlic pollen is free of potyviral infection, the male parents’ plants also need to be protected from contamination, since viral infection weakens plants, reducing flowering ability and pollen production. MDPI 2022-09-21 /pmc/articles/PMC9612218/ /pubmed/36298648 http://dx.doi.org/10.3390/v14102092 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shemesh-Mayer, Einat
Gelbart, Dana
Belausov, Eduard
Sher, Nisan
Daus, Ahuva
Rabinowitch, Haim D.
Kamenetsky-Goldstein, Rina
Garlic Potyviruses Are Translocated to the True Seeds through the Vegetative and Reproductive Systems of the Mother Plant
title Garlic Potyviruses Are Translocated to the True Seeds through the Vegetative and Reproductive Systems of the Mother Plant
title_full Garlic Potyviruses Are Translocated to the True Seeds through the Vegetative and Reproductive Systems of the Mother Plant
title_fullStr Garlic Potyviruses Are Translocated to the True Seeds through the Vegetative and Reproductive Systems of the Mother Plant
title_full_unstemmed Garlic Potyviruses Are Translocated to the True Seeds through the Vegetative and Reproductive Systems of the Mother Plant
title_short Garlic Potyviruses Are Translocated to the True Seeds through the Vegetative and Reproductive Systems of the Mother Plant
title_sort garlic potyviruses are translocated to the true seeds through the vegetative and reproductive systems of the mother plant
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612218/
https://www.ncbi.nlm.nih.gov/pubmed/36298648
http://dx.doi.org/10.3390/v14102092
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