Cargando…
Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait
Parvoviruses are small, single-stranded DNA viruses with non-enveloped capsids. Determining the capsid structures provides a framework for annotating regions important to the viral life cycle. Aleutian mink disease virus (AMDV), a pathogen in minks, and human parvovirus 4 (PARV4), infecting humans,...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612331/ https://www.ncbi.nlm.nih.gov/pubmed/36298773 http://dx.doi.org/10.3390/v14102219 |
_version_ | 1784819748336304128 |
---|---|
author | Lakshmanan, Renuk Mietzsch, Mario Jimenez Ybargollin, Alberto Chipman, Paul Fu, Xiaofeng Qiu, Jianming Söderlund-Venermo, Maria McKenna, Robert |
author_facet | Lakshmanan, Renuk Mietzsch, Mario Jimenez Ybargollin, Alberto Chipman, Paul Fu, Xiaofeng Qiu, Jianming Söderlund-Venermo, Maria McKenna, Robert |
author_sort | Lakshmanan, Renuk |
collection | PubMed |
description | Parvoviruses are small, single-stranded DNA viruses with non-enveloped capsids. Determining the capsid structures provides a framework for annotating regions important to the viral life cycle. Aleutian mink disease virus (AMDV), a pathogen in minks, and human parvovirus 4 (PARV4), infecting humans, are parvoviruses belonging to the genera Amdoparvovirus and Tetraparvovirus, respectively. While Aleutian mink disease caused by AMDV is a major threat to mink farming, no clear clinical manifestations have been established following infection with PARV4 in humans. Here, the capsid structures of AMDV and PARV4 were determined via cryo-electron microscopy at 2.37 and 3.12 Å resolutions, respectively. Despite low amino acid sequence identities (10–30%) both viruses share the icosahedral nature of parvovirus capsids, with 60 viral proteins (VPs) assembling the capsid via two-, three-, and five-fold symmetry VP-related interactions, but display major structural variabilities in the surface loops when the capsid structures are superposed onto other parvoviruses. The capsid structures of AMDV and PARV4 will add to current knowledge of the structural platform for parvoviruses and permit future functional annotation of these viruses, which will help in understanding their infection mechanisms at a molecular level for the development of diagnostics and therapeutics. |
format | Online Article Text |
id | pubmed-9612331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96123312022-10-28 Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait Lakshmanan, Renuk Mietzsch, Mario Jimenez Ybargollin, Alberto Chipman, Paul Fu, Xiaofeng Qiu, Jianming Söderlund-Venermo, Maria McKenna, Robert Viruses Article Parvoviruses are small, single-stranded DNA viruses with non-enveloped capsids. Determining the capsid structures provides a framework for annotating regions important to the viral life cycle. Aleutian mink disease virus (AMDV), a pathogen in minks, and human parvovirus 4 (PARV4), infecting humans, are parvoviruses belonging to the genera Amdoparvovirus and Tetraparvovirus, respectively. While Aleutian mink disease caused by AMDV is a major threat to mink farming, no clear clinical manifestations have been established following infection with PARV4 in humans. Here, the capsid structures of AMDV and PARV4 were determined via cryo-electron microscopy at 2.37 and 3.12 Å resolutions, respectively. Despite low amino acid sequence identities (10–30%) both viruses share the icosahedral nature of parvovirus capsids, with 60 viral proteins (VPs) assembling the capsid via two-, three-, and five-fold symmetry VP-related interactions, but display major structural variabilities in the surface loops when the capsid structures are superposed onto other parvoviruses. The capsid structures of AMDV and PARV4 will add to current knowledge of the structural platform for parvoviruses and permit future functional annotation of these viruses, which will help in understanding their infection mechanisms at a molecular level for the development of diagnostics and therapeutics. MDPI 2022-10-09 /pmc/articles/PMC9612331/ /pubmed/36298773 http://dx.doi.org/10.3390/v14102219 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lakshmanan, Renuk Mietzsch, Mario Jimenez Ybargollin, Alberto Chipman, Paul Fu, Xiaofeng Qiu, Jianming Söderlund-Venermo, Maria McKenna, Robert Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait |
title | Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait |
title_full | Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait |
title_fullStr | Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait |
title_full_unstemmed | Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait |
title_short | Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait |
title_sort | capsid structure of aleutian mink disease virus and human parvovirus 4: new faces in the parvovirus family portrait |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9612331/ https://www.ncbi.nlm.nih.gov/pubmed/36298773 http://dx.doi.org/10.3390/v14102219 |
work_keys_str_mv | AT lakshmananrenuk capsidstructureofaleutianminkdiseasevirusandhumanparvovirus4newfacesintheparvovirusfamilyportrait AT mietzschmario capsidstructureofaleutianminkdiseasevirusandhumanparvovirus4newfacesintheparvovirusfamilyportrait AT jimenezybargollinalberto capsidstructureofaleutianminkdiseasevirusandhumanparvovirus4newfacesintheparvovirusfamilyportrait AT chipmanpaul capsidstructureofaleutianminkdiseasevirusandhumanparvovirus4newfacesintheparvovirusfamilyportrait AT fuxiaofeng capsidstructureofaleutianminkdiseasevirusandhumanparvovirus4newfacesintheparvovirusfamilyportrait AT qiujianming capsidstructureofaleutianminkdiseasevirusandhumanparvovirus4newfacesintheparvovirusfamilyportrait AT soderlundvenermomaria capsidstructureofaleutianminkdiseasevirusandhumanparvovirus4newfacesintheparvovirusfamilyportrait AT mckennarobert capsidstructureofaleutianminkdiseasevirusandhumanparvovirus4newfacesintheparvovirusfamilyportrait |