Expression of CASC8 RNA in Human Pancreatic Cancer Cell Lines

A lot of long non-coding RNAs (lncRNAs) are expressed in human cells in a number of transcripts of different lengths and composition of exons. In case of cancer-associated lncRNAs, an actual task is to determine their specific isoforms, since each transcript can perform its own function in carcinoge...

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Autores principales: Burenina, O. Y., Lazarevich, N. L., Kustova, I. F., Zatsepin, T. S., Rubtsova, M. P., Dontsova, O. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2022
Materias:
Biochemistry, Biophysics, and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9613565/
https://www.ncbi.nlm.nih.gov/pubmed/36038677
http://dx.doi.org/10.1134/S1607672922040020
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author Burenina, O. Y.
Lazarevich, N. L.
Kustova, I. F.
Zatsepin, T. S.
Rubtsova, M. P.
Dontsova, O. A.
author_facet Burenina, O. Y.
Lazarevich, N. L.
Kustova, I. F.
Zatsepin, T. S.
Rubtsova, M. P.
Dontsova, O. A.
author_sort Burenina, O. Y.
collection PubMed
description A lot of long non-coding RNAs (lncRNAs) are expressed in human cells in a number of transcripts of different lengths and composition of exons. In case of cancer-associated lncRNAs, an actual task is to determine their specific isoforms, since each transcript can perform its own function in carcinogenesis and might have a unique expression profile in various types of tumors. For the first time, we analyzed the expression of CASC8 lncRNA in human pancreatic ductal adenocarcinoma cell lines and found an abundant isoform that was previously considered as the minor one in this type of cancer. We also revealed extremely high expression levels of all CASC8 transcripts in MIA PaCa-2 cells and, conversely, the lack of this lncRNA in PANC-1. This allows to use them as convenient models for further in vitro studies.
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spelling pubmed-96135652022-10-29 Expression of CASC8 RNA in Human Pancreatic Cancer Cell Lines Burenina, O. Y. Lazarevich, N. L. Kustova, I. F. Zatsepin, T. S. Rubtsova, M. P. Dontsova, O. A. Dokl Biochem Biophys Biochemistry, Biophysics, and Molecular Biology A lot of long non-coding RNAs (lncRNAs) are expressed in human cells in a number of transcripts of different lengths and composition of exons. In case of cancer-associated lncRNAs, an actual task is to determine their specific isoforms, since each transcript can perform its own function in carcinogenesis and might have a unique expression profile in various types of tumors. For the first time, we analyzed the expression of CASC8 lncRNA in human pancreatic ductal adenocarcinoma cell lines and found an abundant isoform that was previously considered as the minor one in this type of cancer. We also revealed extremely high expression levels of all CASC8 transcripts in MIA PaCa-2 cells and, conversely, the lack of this lncRNA in PANC-1. This allows to use them as convenient models for further in vitro studies. Pleiades Publishing 2022-08-29 2022 /pmc/articles/PMC9613565/ /pubmed/36038677 http://dx.doi.org/10.1134/S1607672922040020 Text en © The Author(s) 2022, ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2022, Vol. 505, pp. 137–140. © The Author(s), 2022. This article is an open access publication, corrected publication 2022.Russian Text © The Author(s), 2022, published in Doklady Rossiiskoi Akademii Nauk. Nauki o Zhizni, 2022, Vol. 505, pp. 253–257. https://creativecommons.org/licenses/by/4.0/Open Access.This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biochemistry, Biophysics, and Molecular Biology
Burenina, O. Y.
Lazarevich, N. L.
Kustova, I. F.
Zatsepin, T. S.
Rubtsova, M. P.
Dontsova, O. A.
Expression of CASC8 RNA in Human Pancreatic Cancer Cell Lines
title Expression of CASC8 RNA in Human Pancreatic Cancer Cell Lines
title_full Expression of CASC8 RNA in Human Pancreatic Cancer Cell Lines
title_fullStr Expression of CASC8 RNA in Human Pancreatic Cancer Cell Lines
title_full_unstemmed Expression of CASC8 RNA in Human Pancreatic Cancer Cell Lines
title_short Expression of CASC8 RNA in Human Pancreatic Cancer Cell Lines
title_sort expression of casc8 rna in human pancreatic cancer cell lines
topic Biochemistry, Biophysics, and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9613565/
https://www.ncbi.nlm.nih.gov/pubmed/36038677
http://dx.doi.org/10.1134/S1607672922040020
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