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Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions
The Toxoplasma gondii tachyzoite is a singled-cell obligate intracellular parasite responsible for the acute phase of toxoplasmosis. This polarized cell exhibits an apical complex, a hallmark of the phylum Apicomplexa, essential for motility, invasion, and egress from the host cell. Located on the o...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9613666/ https://www.ncbi.nlm.nih.gov/pubmed/36310866 http://dx.doi.org/10.3389/fcimb.2022.1010038 |
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author | Roumégous, Chloé Abou Hammoud, Aya Fuster, Damien Dupuy, Jean-William Blancard, Corinne Salin, Bénédicte Robinson, Derrick R. Renesto, Patricia Tardieux, Isabelle Frénal, Karine |
author_facet | Roumégous, Chloé Abou Hammoud, Aya Fuster, Damien Dupuy, Jean-William Blancard, Corinne Salin, Bénédicte Robinson, Derrick R. Renesto, Patricia Tardieux, Isabelle Frénal, Karine |
author_sort | Roumégous, Chloé |
collection | PubMed |
description | The Toxoplasma gondii tachyzoite is a singled-cell obligate intracellular parasite responsible for the acute phase of toxoplasmosis. This polarized cell exhibits an apical complex, a hallmark of the phylum Apicomplexa, essential for motility, invasion, and egress from the host cell. Located on the opposite end of the cell is the basal complex, an elaborated cytoskeletal structure that also plays critical roles in the lytic cycle of the parasite, being involved in motility, cell division, constriction and cytokinesis, as well as intravacuolar cell-cell communication. Nevertheless, only a few proteins of this structure have been described and functionally assessed. In this study, we used spatial proteomics to identify new basal complex components (BCC), and in situ imaging, including ultrastructure expansion microscopy, to position them. We thus confirmed the localization of nine BCCs out of the 12 selected candidates and assigned them to different sub-compartments of the basal complex, including two new domains located above the basal ring and below the posterior cup. Their functional investigation revealed that none of these BCCs are essential for parasite growth in vitro. However, one BCC is critical for constricting of the basal complex, likely through direct interaction with the class VI myosin heavy chain J (MyoJ), and for gliding motility. Four other BCCs, including a phosphatase and a guanylate-binding protein, are involved in the formation and/or maintenance of the intravacuolar parasite connection, which is required for the rosette organization and synchronicity of cell division. |
format | Online Article Text |
id | pubmed-9613666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96136662022-10-29 Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions Roumégous, Chloé Abou Hammoud, Aya Fuster, Damien Dupuy, Jean-William Blancard, Corinne Salin, Bénédicte Robinson, Derrick R. Renesto, Patricia Tardieux, Isabelle Frénal, Karine Front Cell Infect Microbiol Cellular and Infection Microbiology The Toxoplasma gondii tachyzoite is a singled-cell obligate intracellular parasite responsible for the acute phase of toxoplasmosis. This polarized cell exhibits an apical complex, a hallmark of the phylum Apicomplexa, essential for motility, invasion, and egress from the host cell. Located on the opposite end of the cell is the basal complex, an elaborated cytoskeletal structure that also plays critical roles in the lytic cycle of the parasite, being involved in motility, cell division, constriction and cytokinesis, as well as intravacuolar cell-cell communication. Nevertheless, only a few proteins of this structure have been described and functionally assessed. In this study, we used spatial proteomics to identify new basal complex components (BCC), and in situ imaging, including ultrastructure expansion microscopy, to position them. We thus confirmed the localization of nine BCCs out of the 12 selected candidates and assigned them to different sub-compartments of the basal complex, including two new domains located above the basal ring and below the posterior cup. Their functional investigation revealed that none of these BCCs are essential for parasite growth in vitro. However, one BCC is critical for constricting of the basal complex, likely through direct interaction with the class VI myosin heavy chain J (MyoJ), and for gliding motility. Four other BCCs, including a phosphatase and a guanylate-binding protein, are involved in the formation and/or maintenance of the intravacuolar parasite connection, which is required for the rosette organization and synchronicity of cell division. Frontiers Media S.A. 2022-10-13 /pmc/articles/PMC9613666/ /pubmed/36310866 http://dx.doi.org/10.3389/fcimb.2022.1010038 Text en Copyright © 2022 Roumégous, Abou Hammoud, Fuster, Dupuy, Blancard, Salin, Robinson, Renesto, Tardieux and Frénal https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Roumégous, Chloé Abou Hammoud, Aya Fuster, Damien Dupuy, Jean-William Blancard, Corinne Salin, Bénédicte Robinson, Derrick R. Renesto, Patricia Tardieux, Isabelle Frénal, Karine Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions |
title | Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions |
title_full | Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions |
title_fullStr | Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions |
title_full_unstemmed | Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions |
title_short | Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions |
title_sort | identification of new components of the basal pole of toxoplasma gondii provides novel insights into its molecular organization and functions |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9613666/ https://www.ncbi.nlm.nih.gov/pubmed/36310866 http://dx.doi.org/10.3389/fcimb.2022.1010038 |
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