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Genesis of fecal floatation is causally linked to gut microbial colonization in mice
The origin of fecal floatation phenomenon remains poorly understood. Following our serendipitous discovery of differences in buoyancy of feces from germ-free and conventional mice, we characterized microbial and physical properties of feces from germ-free and gut-colonized (conventional and conventi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9613883/ https://www.ncbi.nlm.nih.gov/pubmed/36302811 http://dx.doi.org/10.1038/s41598-022-22626-x |
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author | Aalam, Syed Mohammed Musheer Crasta, Daphne Norma Roy, Pooja Miller, A. Lee Gamb, Scott I. Johnson, Stephen Till, Lisa M. Chen, Jun Kashyap, Purna Kannan, Nagarajan |
author_facet | Aalam, Syed Mohammed Musheer Crasta, Daphne Norma Roy, Pooja Miller, A. Lee Gamb, Scott I. Johnson, Stephen Till, Lisa M. Chen, Jun Kashyap, Purna Kannan, Nagarajan |
author_sort | Aalam, Syed Mohammed Musheer |
collection | PubMed |
description | The origin of fecal floatation phenomenon remains poorly understood. Following our serendipitous discovery of differences in buoyancy of feces from germ-free and conventional mice, we characterized microbial and physical properties of feces from germ-free and gut-colonized (conventional and conventionalized) mice. The gut-colonization associated differences were assessed in feces using DNA, bacterial-PCR, scanning electron microscopy, FACS, thermogravimetry and pycnometry. Based on the differences in buoyancy of feces, we developed levô in fimo test (LIFT) to distinguish sinking feces (sinkers) of germ-free mice from floating feces (floaters) of gut-colonized mice. By simultaneous tracking of microbiota densities and gut colonization kinetics in fecal transplanted mice, we provide first direct evidence of causal relationship between gut microbial colonization and fecal floatation. Rare discordance in LIFT and microbiota density indicated that enrichment of gasogenic gut colonizers may be necessary for fecal floatation. Finally, fecal metagenomics analysis of ‘floaters’ from conventional and syngeneic fecal transplanted mice identified colonization of > 10 gasogenic bacterial species including highly prevalent B. ovatus, an anaerobic commensal bacteria linked with flatulence and intestinal bowel diseases. The findings reported here will improve our understanding of food microbial biotransformation and gut microbial regulators of fecal floatation in human health and disease. |
format | Online Article Text |
id | pubmed-9613883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96138832022-10-29 Genesis of fecal floatation is causally linked to gut microbial colonization in mice Aalam, Syed Mohammed Musheer Crasta, Daphne Norma Roy, Pooja Miller, A. Lee Gamb, Scott I. Johnson, Stephen Till, Lisa M. Chen, Jun Kashyap, Purna Kannan, Nagarajan Sci Rep Article The origin of fecal floatation phenomenon remains poorly understood. Following our serendipitous discovery of differences in buoyancy of feces from germ-free and conventional mice, we characterized microbial and physical properties of feces from germ-free and gut-colonized (conventional and conventionalized) mice. The gut-colonization associated differences were assessed in feces using DNA, bacterial-PCR, scanning electron microscopy, FACS, thermogravimetry and pycnometry. Based on the differences in buoyancy of feces, we developed levô in fimo test (LIFT) to distinguish sinking feces (sinkers) of germ-free mice from floating feces (floaters) of gut-colonized mice. By simultaneous tracking of microbiota densities and gut colonization kinetics in fecal transplanted mice, we provide first direct evidence of causal relationship between gut microbial colonization and fecal floatation. Rare discordance in LIFT and microbiota density indicated that enrichment of gasogenic gut colonizers may be necessary for fecal floatation. Finally, fecal metagenomics analysis of ‘floaters’ from conventional and syngeneic fecal transplanted mice identified colonization of > 10 gasogenic bacterial species including highly prevalent B. ovatus, an anaerobic commensal bacteria linked with flatulence and intestinal bowel diseases. The findings reported here will improve our understanding of food microbial biotransformation and gut microbial regulators of fecal floatation in human health and disease. Nature Publishing Group UK 2022-10-27 /pmc/articles/PMC9613883/ /pubmed/36302811 http://dx.doi.org/10.1038/s41598-022-22626-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Aalam, Syed Mohammed Musheer Crasta, Daphne Norma Roy, Pooja Miller, A. Lee Gamb, Scott I. Johnson, Stephen Till, Lisa M. Chen, Jun Kashyap, Purna Kannan, Nagarajan Genesis of fecal floatation is causally linked to gut microbial colonization in mice |
title | Genesis of fecal floatation is causally linked to gut microbial colonization in mice |
title_full | Genesis of fecal floatation is causally linked to gut microbial colonization in mice |
title_fullStr | Genesis of fecal floatation is causally linked to gut microbial colonization in mice |
title_full_unstemmed | Genesis of fecal floatation is causally linked to gut microbial colonization in mice |
title_short | Genesis of fecal floatation is causally linked to gut microbial colonization in mice |
title_sort | genesis of fecal floatation is causally linked to gut microbial colonization in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9613883/ https://www.ncbi.nlm.nih.gov/pubmed/36302811 http://dx.doi.org/10.1038/s41598-022-22626-x |
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