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Transcriptomic analysis of the anti-inflammatory effect of Cordyceps militaris extract on acute gouty arthritis
Background: Gouty arthritis (GA) is a common inflammatory disease that causes pain due to the deposition of monosodium urate (MSU) crystals into joints and surrounding tissues. Anti-inflammatory drugs have significant clinical anti-inflammatory and analgesic effects, but they have many side effects....
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614083/ https://www.ncbi.nlm.nih.gov/pubmed/36313318 http://dx.doi.org/10.3389/fphar.2022.1035101 |
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author | Jiao, Chunwei Liang, Huijia Liu, Li Li, Shunxian Chen, Jiaming Xie, Yizhen |
author_facet | Jiao, Chunwei Liang, Huijia Liu, Li Li, Shunxian Chen, Jiaming Xie, Yizhen |
author_sort | Jiao, Chunwei |
collection | PubMed |
description | Background: Gouty arthritis (GA) is a common inflammatory disease that causes pain due to the deposition of monosodium urate (MSU) crystals into joints and surrounding tissues. Anti-inflammatory drugs have significant clinical anti-inflammatory and analgesic effects, but they have many side effects. Cordyceps militaris is an edible and medicinal fungus, and its extract (CME) has good anti-inflammatory and analgesic effects. This study aimed to investigate the anti-inflammatory effect of CME on GA and its underlying mechanism. Methods: The effect of CME on the expression of related inflammatory factors and histopathological changes in the MSU-induced acute inflammatory gout model in rats was studied by ELISA and HE, and its anti-inflammatory mechanism was analyzed by transcriptome combined with RT-qPCR. Results: CME significantly improved gait scores and joint swelling in GA rats, and reduced MSU-induced inflammatory cell infiltration. CME inhibited MSU-induced inflammatory responses by reducing the levels of pro-inflammatory factors TNF-α, IL-1β, IL-6, and Caspase-1 and increasing the anti-inflammatory factor IL-10. Transcriptome analysis showed that CME significantly altered inflammation-related cytokine pathways, and identified four major genes involved in regulation of inflammation, CCL7, CSF2RB, LIF, and IL-1β. In addition, RT-qPCR was performed to verify these differential genes. Conclusion: CME significantly alleviated the inflammatory progression of GA and ameliorated the onset of GA. The underlying mechanism may be related to triggering the cytokine-cytokine receptor interaction signaling pathway to inhibit the activation of the inflammasome and regulate the immune system. And it regulates the inflammatory response induced by MSU crystals through the genes CCL7, CSF2RB, and IL-1β. |
format | Online Article Text |
id | pubmed-9614083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96140832022-10-29 Transcriptomic analysis of the anti-inflammatory effect of Cordyceps militaris extract on acute gouty arthritis Jiao, Chunwei Liang, Huijia Liu, Li Li, Shunxian Chen, Jiaming Xie, Yizhen Front Pharmacol Pharmacology Background: Gouty arthritis (GA) is a common inflammatory disease that causes pain due to the deposition of monosodium urate (MSU) crystals into joints and surrounding tissues. Anti-inflammatory drugs have significant clinical anti-inflammatory and analgesic effects, but they have many side effects. Cordyceps militaris is an edible and medicinal fungus, and its extract (CME) has good anti-inflammatory and analgesic effects. This study aimed to investigate the anti-inflammatory effect of CME on GA and its underlying mechanism. Methods: The effect of CME on the expression of related inflammatory factors and histopathological changes in the MSU-induced acute inflammatory gout model in rats was studied by ELISA and HE, and its anti-inflammatory mechanism was analyzed by transcriptome combined with RT-qPCR. Results: CME significantly improved gait scores and joint swelling in GA rats, and reduced MSU-induced inflammatory cell infiltration. CME inhibited MSU-induced inflammatory responses by reducing the levels of pro-inflammatory factors TNF-α, IL-1β, IL-6, and Caspase-1 and increasing the anti-inflammatory factor IL-10. Transcriptome analysis showed that CME significantly altered inflammation-related cytokine pathways, and identified four major genes involved in regulation of inflammation, CCL7, CSF2RB, LIF, and IL-1β. In addition, RT-qPCR was performed to verify these differential genes. Conclusion: CME significantly alleviated the inflammatory progression of GA and ameliorated the onset of GA. The underlying mechanism may be related to triggering the cytokine-cytokine receptor interaction signaling pathway to inhibit the activation of the inflammasome and regulate the immune system. And it regulates the inflammatory response induced by MSU crystals through the genes CCL7, CSF2RB, and IL-1β. Frontiers Media S.A. 2022-10-14 /pmc/articles/PMC9614083/ /pubmed/36313318 http://dx.doi.org/10.3389/fphar.2022.1035101 Text en Copyright © 2022 Jiao, Liang, Liu, Li, Chen and Xie. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Jiao, Chunwei Liang, Huijia Liu, Li Li, Shunxian Chen, Jiaming Xie, Yizhen Transcriptomic analysis of the anti-inflammatory effect of Cordyceps militaris extract on acute gouty arthritis |
title | Transcriptomic analysis of the anti-inflammatory effect of Cordyceps militaris extract on acute gouty arthritis |
title_full | Transcriptomic analysis of the anti-inflammatory effect of Cordyceps militaris extract on acute gouty arthritis |
title_fullStr | Transcriptomic analysis of the anti-inflammatory effect of Cordyceps militaris extract on acute gouty arthritis |
title_full_unstemmed | Transcriptomic analysis of the anti-inflammatory effect of Cordyceps militaris extract on acute gouty arthritis |
title_short | Transcriptomic analysis of the anti-inflammatory effect of Cordyceps militaris extract on acute gouty arthritis |
title_sort | transcriptomic analysis of the anti-inflammatory effect of cordyceps militaris extract on acute gouty arthritis |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614083/ https://www.ncbi.nlm.nih.gov/pubmed/36313318 http://dx.doi.org/10.3389/fphar.2022.1035101 |
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