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Long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats
BACKGROUND: The brain-gut axis is a major regulator of the central nervous system. We investigated the effects of treatment with broad-spectrum antibiotics on gut and brain inflammation, infarct size and long-term behavioral outcome after cerebral ischemia in rats. METHODS: Rats were treated with br...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BMJ Publishing Group
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614136/ https://www.ncbi.nlm.nih.gov/pubmed/35577395 http://dx.doi.org/10.1136/svn-2021-001231 |
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author | Liu, Chang Cheng, Xi Zhong, Shanshan Liu, Zhouyang Liu, Fangxi Lin, Xinyu Zhao, Yinan Guan, Meiting Xiao, Ting Jolkkonen, Jukka Wang, Ying Zhao, Chuansheng |
author_facet | Liu, Chang Cheng, Xi Zhong, Shanshan Liu, Zhouyang Liu, Fangxi Lin, Xinyu Zhao, Yinan Guan, Meiting Xiao, Ting Jolkkonen, Jukka Wang, Ying Zhao, Chuansheng |
author_sort | Liu, Chang |
collection | PubMed |
description | BACKGROUND: The brain-gut axis is a major regulator of the central nervous system. We investigated the effects of treatment with broad-spectrum antibiotics on gut and brain inflammation, infarct size and long-term behavioral outcome after cerebral ischemia in rats. METHODS: Rats were treated with broad-spectrum antibiotics (ampicillin, vancomycin, ciprofloxacin, meropenem and metronidazole) for 4 weeks before the endothelin-1 induced ischemia. Treatment continued for 2 weeks until the end of behavioral testing, which included tapered ledged beam-walking, adhesive label test and cylinder test. Gut microbiome, short-chain fatty acids and cytokine levels were measured together with an assessment of infarct size, neuroinflammation and neurogenesis. RESULTS: The results revealed that the antibiotics exerted a clear impact on the gut microbiota. This was associated with a decrease in systemic and brain cytokine levels, infarct size and apoptosis in the perilesional cortex and improved behavioral outcome. CONCLUSION: Our results highlighted the significant relationship between intestinal microbiota and beneficial neuro-recovery after ischemic stroke. |
format | Online Article Text |
id | pubmed-9614136 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BMJ Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-96141362022-10-29 Long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats Liu, Chang Cheng, Xi Zhong, Shanshan Liu, Zhouyang Liu, Fangxi Lin, Xinyu Zhao, Yinan Guan, Meiting Xiao, Ting Jolkkonen, Jukka Wang, Ying Zhao, Chuansheng Stroke Vasc Neurol Original Research BACKGROUND: The brain-gut axis is a major regulator of the central nervous system. We investigated the effects of treatment with broad-spectrum antibiotics on gut and brain inflammation, infarct size and long-term behavioral outcome after cerebral ischemia in rats. METHODS: Rats were treated with broad-spectrum antibiotics (ampicillin, vancomycin, ciprofloxacin, meropenem and metronidazole) for 4 weeks before the endothelin-1 induced ischemia. Treatment continued for 2 weeks until the end of behavioral testing, which included tapered ledged beam-walking, adhesive label test and cylinder test. Gut microbiome, short-chain fatty acids and cytokine levels were measured together with an assessment of infarct size, neuroinflammation and neurogenesis. RESULTS: The results revealed that the antibiotics exerted a clear impact on the gut microbiota. This was associated with a decrease in systemic and brain cytokine levels, infarct size and apoptosis in the perilesional cortex and improved behavioral outcome. CONCLUSION: Our results highlighted the significant relationship between intestinal microbiota and beneficial neuro-recovery after ischemic stroke. BMJ Publishing Group 2022-05-16 /pmc/articles/PMC9614136/ /pubmed/35577395 http://dx.doi.org/10.1136/svn-2021-001231 Text en © Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Original Research Liu, Chang Cheng, Xi Zhong, Shanshan Liu, Zhouyang Liu, Fangxi Lin, Xinyu Zhao, Yinan Guan, Meiting Xiao, Ting Jolkkonen, Jukka Wang, Ying Zhao, Chuansheng Long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats |
title | Long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats |
title_full | Long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats |
title_fullStr | Long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats |
title_full_unstemmed | Long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats |
title_short | Long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats |
title_sort | long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614136/ https://www.ncbi.nlm.nih.gov/pubmed/35577395 http://dx.doi.org/10.1136/svn-2021-001231 |
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