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Optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production

Common carp (Cyprinus carpio) is the fourth most-produced fish species in aquaculture and frequently used model species with significant effort invested in development of biotechnological applications. In present study, we attempted to establish an in vitro germ cell culture condition for short term...

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Autores principales: Xie, Xuan, Franěk, Roman, Pšenička, Martin, Chen, Fan, Kašpar, Vojtech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614374/
https://www.ncbi.nlm.nih.gov/pubmed/36311648
http://dx.doi.org/10.3389/fvets.2022.1036495
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author Xie, Xuan
Franěk, Roman
Pšenička, Martin
Chen, Fan
Kašpar, Vojtech
author_facet Xie, Xuan
Franěk, Roman
Pšenička, Martin
Chen, Fan
Kašpar, Vojtech
author_sort Xie, Xuan
collection PubMed
description Common carp (Cyprinus carpio) is the fourth most-produced fish species in aquaculture and frequently used model species with significant effort invested in development of biotechnological applications. In present study, we attempted to establish an in vitro germ cell culture condition for short term cell culture, which could facilitate further applications such as surrogacy or gene manipulation. Basal media and different types of feeder cells were investigated to optimize carp germ cell culture condition to favor maintenance of mitotic proliferation. Results indicated that germ cells cultured with hESC media and RTG2 cell line as feeder possessed significantly higher proliferation and survival rate compared to that cultured with StemPro media and Sertoli cell line as feeder. In addition, we compared two dissection strategies to compare risk of cell culture contamination and body cavity was open from dorsal part or from ventral part. As a result, carp open from the dorsal side can minimize the risk of contamination. In summary, this is the first study to optimize the cultivation of germ cells in common carp. This opens up new opportunities for the application of specific techniques in the breeding of those species with high commercial value and frequent use as a model fish. Results obtained in this study are important for implementation of new strategies in common carp breeding, conservation of genetic resources, restoration of lines or development of clonal and isogenic carp lines.
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spelling pubmed-96143742022-10-29 Optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production Xie, Xuan Franěk, Roman Pšenička, Martin Chen, Fan Kašpar, Vojtech Front Vet Sci Veterinary Science Common carp (Cyprinus carpio) is the fourth most-produced fish species in aquaculture and frequently used model species with significant effort invested in development of biotechnological applications. In present study, we attempted to establish an in vitro germ cell culture condition for short term cell culture, which could facilitate further applications such as surrogacy or gene manipulation. Basal media and different types of feeder cells were investigated to optimize carp germ cell culture condition to favor maintenance of mitotic proliferation. Results indicated that germ cells cultured with hESC media and RTG2 cell line as feeder possessed significantly higher proliferation and survival rate compared to that cultured with StemPro media and Sertoli cell line as feeder. In addition, we compared two dissection strategies to compare risk of cell culture contamination and body cavity was open from dorsal part or from ventral part. As a result, carp open from the dorsal side can minimize the risk of contamination. In summary, this is the first study to optimize the cultivation of germ cells in common carp. This opens up new opportunities for the application of specific techniques in the breeding of those species with high commercial value and frequent use as a model fish. Results obtained in this study are important for implementation of new strategies in common carp breeding, conservation of genetic resources, restoration of lines or development of clonal and isogenic carp lines. Frontiers Media S.A. 2022-10-14 /pmc/articles/PMC9614374/ /pubmed/36311648 http://dx.doi.org/10.3389/fvets.2022.1036495 Text en Copyright © 2022 Xie, Franěk, Pšenička, Chen and Kašpar. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Xie, Xuan
Franěk, Roman
Pšenička, Martin
Chen, Fan
Kašpar, Vojtech
Optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production
title Optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production
title_full Optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production
title_fullStr Optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production
title_full_unstemmed Optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production
title_short Optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production
title_sort optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614374/
https://www.ncbi.nlm.nih.gov/pubmed/36311648
http://dx.doi.org/10.3389/fvets.2022.1036495
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