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DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis
Flagellin perception is a keystone of pattern-triggered immunity in plants. The recognition of this protein by a plasma membrane (PM) receptor complex is the beginning of a signaling cascade that includes protein phosphorylation and the production of reactive oxygen species (ROS). In both Arabidopsi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614507/ https://www.ncbi.nlm.nih.gov/pubmed/35900211 http://dx.doi.org/10.1093/plphys/kiac354 |
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author | Kalachova, Tetiana Škrabálková, Eliška Pateyron, Stéphanie Soubigou-Taconnat, Ludivine Djafi, Nabila Collin, Sylvie Sekereš, Juraj Burketová, Lenka Potocký, Martin Pejchar, Přemysl Ruelland, Eric |
author_facet | Kalachova, Tetiana Škrabálková, Eliška Pateyron, Stéphanie Soubigou-Taconnat, Ludivine Djafi, Nabila Collin, Sylvie Sekereš, Juraj Burketová, Lenka Potocký, Martin Pejchar, Přemysl Ruelland, Eric |
author_sort | Kalachova, Tetiana |
collection | PubMed |
description | Flagellin perception is a keystone of pattern-triggered immunity in plants. The recognition of this protein by a plasma membrane (PM) receptor complex is the beginning of a signaling cascade that includes protein phosphorylation and the production of reactive oxygen species (ROS). In both Arabidopsis (Arabidopsis thaliana) seedlings and suspension cells, we found that treatment with flg22, a peptide corresponding to the most conserved domain of bacterial flagellin, caused a rapid and transient decrease in the level of phosphatidylinositol (PI) 4,5-bisphosphate along with a parallel increase in phosphatidic acid (PA). In suspension cells, inhibitors of either phosphoinositide-dependent phospholipases C (PLC) or diacylglycerol kinases (DGKs) inhibited flg22-triggered PA production and the oxidative burst. In response to flg22, receptor-like kinase-deficient fls2, bak1, and bik1 mutants (FLAGELLIN SENSITIVE 2, BRASSINOSTEROID INSENSITIVE 1-associated kinase 1, and BOTRYTIS-INDUCED KINASE 1, respectively) produced less PA than wild-type (WT) plants, whereas this response did not differ in NADPH oxidase-deficient rbohD (RESPIRATORY BURST OXIDASE HOMOLOG D) plants. Among the DGK-deficient lines tested, the dgk5.1 mutant produced less PA and less ROS after flg22 treatment compared with WT seedlings. In response to flg22, dgk5.1 plants showed lower callose accumulation and impaired resistance to Pseudomonas syringae pv. tomato DC3000 hrcC-. Transcriptomics revealed that the basal expression of defense-related genes was altered in dgk5.1 seedlings compared with the WT. A GFP-DGK5 fusion protein localized to the PM, where RBOHD and PLC2 (proteins involved in plant immunity) are also located. The role of DGK5 and its enzymatic activity in flagellin signaling and fine-tuning of early immune responses in plant–microbe interactions is discussed. |
format | Online Article Text |
id | pubmed-9614507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-96145072022-11-01 DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis Kalachova, Tetiana Škrabálková, Eliška Pateyron, Stéphanie Soubigou-Taconnat, Ludivine Djafi, Nabila Collin, Sylvie Sekereš, Juraj Burketová, Lenka Potocký, Martin Pejchar, Přemysl Ruelland, Eric Plant Physiol Research Articles Flagellin perception is a keystone of pattern-triggered immunity in plants. The recognition of this protein by a plasma membrane (PM) receptor complex is the beginning of a signaling cascade that includes protein phosphorylation and the production of reactive oxygen species (ROS). In both Arabidopsis (Arabidopsis thaliana) seedlings and suspension cells, we found that treatment with flg22, a peptide corresponding to the most conserved domain of bacterial flagellin, caused a rapid and transient decrease in the level of phosphatidylinositol (PI) 4,5-bisphosphate along with a parallel increase in phosphatidic acid (PA). In suspension cells, inhibitors of either phosphoinositide-dependent phospholipases C (PLC) or diacylglycerol kinases (DGKs) inhibited flg22-triggered PA production and the oxidative burst. In response to flg22, receptor-like kinase-deficient fls2, bak1, and bik1 mutants (FLAGELLIN SENSITIVE 2, BRASSINOSTEROID INSENSITIVE 1-associated kinase 1, and BOTRYTIS-INDUCED KINASE 1, respectively) produced less PA than wild-type (WT) plants, whereas this response did not differ in NADPH oxidase-deficient rbohD (RESPIRATORY BURST OXIDASE HOMOLOG D) plants. Among the DGK-deficient lines tested, the dgk5.1 mutant produced less PA and less ROS after flg22 treatment compared with WT seedlings. In response to flg22, dgk5.1 plants showed lower callose accumulation and impaired resistance to Pseudomonas syringae pv. tomato DC3000 hrcC-. Transcriptomics revealed that the basal expression of defense-related genes was altered in dgk5.1 seedlings compared with the WT. A GFP-DGK5 fusion protein localized to the PM, where RBOHD and PLC2 (proteins involved in plant immunity) are also located. The role of DGK5 and its enzymatic activity in flagellin signaling and fine-tuning of early immune responses in plant–microbe interactions is discussed. Oxford University Press 2022-07-28 /pmc/articles/PMC9614507/ /pubmed/35900211 http://dx.doi.org/10.1093/plphys/kiac354 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Kalachova, Tetiana Škrabálková, Eliška Pateyron, Stéphanie Soubigou-Taconnat, Ludivine Djafi, Nabila Collin, Sylvie Sekereš, Juraj Burketová, Lenka Potocký, Martin Pejchar, Přemysl Ruelland, Eric DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis |
title | DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis |
title_full | DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis |
title_fullStr | DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis |
title_full_unstemmed | DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis |
title_short | DIACYLGLYCEROL KINASE 5 participates in flagellin-induced signaling in Arabidopsis |
title_sort | diacylglycerol kinase 5 participates in flagellin-induced signaling in arabidopsis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614507/ https://www.ncbi.nlm.nih.gov/pubmed/35900211 http://dx.doi.org/10.1093/plphys/kiac354 |
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