Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)

Hypoplastic left heart syndrome (HLHS) is a severe congenital heart defect characterized by underdeveloped structures on the left side of the heart, including hypoplasia of the left ventricle and stenosis or atresia of the aortic and mitral valves. Here, we generated an iPSC line from the peripheral...

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Autores principales: Alonzo, Matthew, Contreras, Javier, Ye, Shiqiao, Lin, Hui, Hernandez-Rosario, Lumariz, McBride, Kim L., Texter, Karen, Garg, Vidu, Zhao, Ming-Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614611/
https://www.ncbi.nlm.nih.gov/pubmed/35987121
http://dx.doi.org/10.1016/j.scr.2022.102892
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author Alonzo, Matthew
Contreras, Javier
Ye, Shiqiao
Lin, Hui
Hernandez-Rosario, Lumariz
McBride, Kim L.
Texter, Karen
Garg, Vidu
Zhao, Ming-Tao
author_facet Alonzo, Matthew
Contreras, Javier
Ye, Shiqiao
Lin, Hui
Hernandez-Rosario, Lumariz
McBride, Kim L.
Texter, Karen
Garg, Vidu
Zhao, Ming-Tao
author_sort Alonzo, Matthew
collection PubMed
description Hypoplastic left heart syndrome (HLHS) is a severe congenital heart defect characterized by underdeveloped structures on the left side of the heart, including hypoplasia of the left ventricle and stenosis or atresia of the aortic and mitral valves. Here, we generated an iPSC line from the peripheral blood mononuclear cells of a male patient with HLHS through Sendai virus-mediated transfection of 4 Yamanaka factors. This iPSC line exhibited normal morphology, expressed pluripotency markers, had a normal karyotype, and could differentiate into cells of three germ layers. This iPSC line can be used for studying cellular and developmental etiologies of HLHS.
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spelling pubmed-96146112022-10-28 Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS) Alonzo, Matthew Contreras, Javier Ye, Shiqiao Lin, Hui Hernandez-Rosario, Lumariz McBride, Kim L. Texter, Karen Garg, Vidu Zhao, Ming-Tao Stem Cell Res Article Hypoplastic left heart syndrome (HLHS) is a severe congenital heart defect characterized by underdeveloped structures on the left side of the heart, including hypoplasia of the left ventricle and stenosis or atresia of the aortic and mitral valves. Here, we generated an iPSC line from the peripheral blood mononuclear cells of a male patient with HLHS through Sendai virus-mediated transfection of 4 Yamanaka factors. This iPSC line exhibited normal morphology, expressed pluripotency markers, had a normal karyotype, and could differentiate into cells of three germ layers. This iPSC line can be used for studying cellular and developmental etiologies of HLHS. 2022-10 2022-08-09 /pmc/articles/PMC9614611/ /pubmed/35987121 http://dx.doi.org/10.1016/j.scr.2022.102892 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Alonzo, Matthew
Contreras, Javier
Ye, Shiqiao
Lin, Hui
Hernandez-Rosario, Lumariz
McBride, Kim L.
Texter, Karen
Garg, Vidu
Zhao, Ming-Tao
Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)
title Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)
title_full Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)
title_fullStr Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)
title_full_unstemmed Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)
title_short Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)
title_sort characterization of an ipsc line nchi006-a from a patient with hypoplastic left heart syndrome (hlhs)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614611/
https://www.ncbi.nlm.nih.gov/pubmed/35987121
http://dx.doi.org/10.1016/j.scr.2022.102892
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